RESUMO
Objective: To investigate effects of intraperitoneally administration of alpha-tocopherol loaded nanoparticles [TNP] on ischemia-reperfusion injury in ovaries
Methods: Thirty-five healthy female Wistar rats 250g were randomized into seven experimental groups [n=5]: Group SHAM: The rats underwent only laparotomy. Group Ischemia: A 3- hour ischemia only. Group I/R: A 3-hour ischemia and a 3-hour reperfusion. Group I/T: A 3-hour ischemia only and 100 mg/kg intraperitoneal administration [IP] of alpha-tocopherol 2.5 hours after induction of ischemia. Group I/R/T: A 3-hour ischemia, a 3-hour reperfusion and 100 mg/kg IP of alpha-tocopherol 2.5 hours after induction of ischemia. Group I/TNP: A 3-hour ischemia only and 1 mg/kg IP of TNP 2.5 hours after induction of ischemia. Group I/R/TNP: A 3-hour ischemia, a 3-hour reperfusion and 1 mg/kg IP of TNP 2.5 hours after induction of ischemia
Results: Animals treated with alpha TNP showed significantly ameliorated development of ischemia and reperfusion tissue injury compared to those of other groups [p=0.001]. The significant higher values of SOD, tGSH, GPO, GSHRd and GST were observed in I/R/NC animals compared to those of other groups [p=0.001]. Damage indicators [NOS, MDA, MPO and DNA damage level] were significantly lower in I/R/NC animal compared to those of other groups [p=0.001]
Conclusion: Intraperitoneal administration of TNP could be helpful in minimizing ischemia-reperfusion injury in ovarian tissue exposed to ischemia