Physical activity and influencing factors of preschool children in Nanchang / 中国学校卫生

Objective@#To understand the present situation of physical activity and its influencing factors, and to provide a reference for improving the level of physical activity and making the intervention measures.@*Methods@#The method of random stratification was used to select 4 740 pre school children aged 3-6 from 17 kindergartens in 12 counties and districts of Nanchang City, The questionnaire of physical activities of young children and the questionnaire of parents of physical activities of young children were applied to conduct a survey.@*Results@#Compared with weekday PA and MVPA, preschool children’s weekends decreased, SB increased. The differences in PA, MVPA and SB on weekdays and weekends were statistically significant( P <0.01). The proportion of PA and MVPA reaching the recommended amount during the working day of preschool children were 44.9%-59.2%, 45.4%- 61.7%.The proportion reaching the recommended amount of PA and MVPA on weekends were 24.7%-27.8%, 24.5%-29.9%, and the proportion reaching the recommended amount on weekdays was higher than that on weekends.@*Conclusion@#There is still gap between actual amount of physical activity and the recommended amount. There are different modes of activity on weekdays and weekends, and weekends are the least active periods. Parents and teachers should pay enough attention to the establishment of "social campus family" model to improve the lack of physical activity of preschool children.

The effects of microRNA-1224-5p on hepatocellular carcinoma tumor endothelial cells

Aim: The aim of this study was to investigate the effect of microRNA-1224-5p (miR-1224-5p) on tumor endothelial cells (TECs) of human hepatocellular carcinoma (HCC). Subjects and Methods: Oligonucleotides were chemically synthesized and transfected into TECs using Lipofectamine 2000. TECs were divided into three groups, namely a control (CON) group without transfection, a negative control (NC) group transfected with negative control oligonucleotides and green fluorescent protein (GFP), and a micro-up (MU) group transfected with miR-1224-5p mimic and GFP. The expression of miR-1224-5p was quantified via quantitative reverse-transcription polymerase chain reaction (qRT-PCR). The proliferation of TECs was detected using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the optical density value at 490 nm was measured after every 24 h. Apoptosis was detected via flow cytometry using a 7-aminoactinomycin/APC Annexin V kit. The migration and invasion of TECs were detected using transwell assay. The tube formation ability was evaluated using the tube formation assay. Results: Oligonucleotides were successfully transduced into TECs, and the expression of miR-1224-5p was specifically upregulated. The results of qRT-PCR analysis showed that the expression of miR-1224-5p was significantly upregulated in the MU group (2–ΔΔCt = 3.27 ± 0.15) than in the CON group (2–ΔΔCt = 1) and NC group (2–ΔΔCt = 1.08 ± 0.11) (P < 0.01). The results of MTT assay showed that the cell proliferation was significantly inhibited in the MU group at four time points than in the CON and NC groups (P < 0.01). Flow cytometry analysis revealed the significant increase in apoptosis of cells from the MU group (19.29% ± 0.95%) than those from the CON (8.73% ± 0.64%) and NC (9.51% ± 0.56%) (P < 0.01) groups. The migration ability was significantly inhibited in MU group (51.0 ± 3.6) as compared with CON (77.7 ± 2.5) and NC (79.2 ± 3.5) groups (P < 0.01). The invasion ability of TECs was significantly inhibited in MU group (9.8 ± 1.3) than in CON (15.8 ± 0.8) and NC (15.4 ± 0.9) groups (P < 0.01). The ability of tube formation of TECs was completely inhibited in MU group but remained unaffected in CON and NC groups. Conclusions: miR-1224-5p may serve as a potential tumor suppressor in HCC. Upregulation in miR-1224-5p expression may decrease proliferation, induce apoptosis, inhibit migration and invasion, and suppress tube formation in TECs of human HCC

Risk factors associated with clinical prognosis of cervical cancer treated with laparoscopic surgery / 中国内镜杂志

Objective To investigate the risk factors associated with clinical prognosis of cervical cancer treated with laparoscopic surgery. Methods 80 patients with cervical cancer were recruited in the study, who underwent radical surgery with laparoscopic surgery from March 2013 to December 2016. The patients were followed up after surgery, and the overall survival and disease free survival was analyzed with tumor recurrence and death as the terminal events. And the risk factors associated with clinical prognosis were identified by using Cox regression analysis. Results The patients were followed up from 12 months to 46 months, and the median period was 39 months. There were 16 recurrences and 6 deaths during the period of follow-up, yielding a disease-free survival of (41.85 ± 1.06) year and an overall survival of (44.86 ± 0.74) year. Cox regression analysis demonstrated that tumor size, clinical stage, lymph node metastasis and vascular invasion were independent risk factors associated with clinical prognosis of cervical cancer treated with laparoscopic surgery. Conclusion Tumor size, clinical stage, lymph node metastasis and vascular invasion were independent risk factors associated with clinical prognosis of cervical cancer treated with laparoscopic surgery.

Risk factors associated with clinical prognosis of cervical cancer treated with laparoscopic surgery / 中国内镜杂志

Objective To investigate the risk factors associated with clinical prognosis of cervical cancer treated with laparoscopic surgery. Methods 80 patients with cervical cancer were recruited in the study, who underwent radical surgery with laparoscopic surgery from March 2013 to December 2016. The patients were followed up after surgery, and the overall survival and disease free survival was analyzed with tumor recurrence and death as the terminal events. And the risk factors associated with clinical prognosis were identified by using Cox regression analysis. Results The patients were followed up from 12 months to 46 months, and the median period was 39 months. There were 16 recurrences and 6 deaths during the period of follow-up, yielding a disease-free survival of (41.85 ± 1.06) year and an overall survival of (44.86 ± 0.74) year. Cox regression analysis demonstrated that tumor size, clinical stage, lymph node metastasis and vascular invasion were independent risk factors associated with clinical prognosis of cervical cancer treated with laparoscopic surgery. Conclusion Tumor size, clinical stage, lymph node metastasis and vascular invasion were independent risk factors associated with clinical prognosis of cervical cancer treated with laparoscopic surgery.

Advances in studies on growth metabolism and response mechanisms of medicinal plants under drought stress / 中国中药杂志

Drought stress exerts a considerable effect on growth, physiology and secondary metabolisms of the medicinal plants. It could inhabit the growth of the medicinal plants but promote secretion of secondary metabolites. Other researches indicated that the medicinal plants could depend on the ABA signaling pathway and secreting osmotic substances to resist the drought stress and reduce the damage by it. The article concludes the changes in growth, physiology, secondary metabolisms and response mechanisms of medicinal plants to drought stress that provides a theoretical basis for exploring the relationship between medicinal plants and drought stress.

Genetic diversity of different populations of lilyturf revealed by RSAP analysis / 中国中药杂志

Restriction site amplification polymorphism (RSAP) markers were employed to access the genetic diversity and relationship of 120 lilyturf germplasms from different geographical origins. Sixteen RSAP primer pairs generated 326 polymorphic bands, of which 318 (97.55%) were polymorphic. The value of polymorphism information content (PIC) ranged from 0.87 to 0.95 with an average of 0.92. These results indicated there was abundant genetic diversity among samples. The results of data analysis on 20 population showed that the value of percentage of polymorphic locus (PPL), Nei's gene diversity (H) and Shannon's information index (I) were 19.94%-85.58%, 0.082 6-0.210 7, 0.120 6-0.328 1 respectively. The most abundant genetic diversity was found in the O. japonicus population from Zhejiang and the least in the Liriope minor population. The genetic distance among 20 population was 0.024 6-0.286 8, of which the minimum genetic distance was 0.024 6 between population I and population 13 while the maximum 0.286 8 between population 5 and population 15. Coefficient of genetic differentiation among natural populations was 0.115 3 (Gst). And the gene differentiation contributed to 43.07% of the total genetic variation among populations and to 56.93% within populations. The total gene flow (Nm) was 0.660 9. UPMGA clustering analysis was basically similar to of the principle coordinate analysis (PCA). The 120 samples were classified into four major groups, which were basically corresponded with the genetic relationships based on morphological traits. The results of UPMGA and PCA were also consistent with geographical origins.

Bmil gene in stem cells and tumorigenesis / 国际肿瘤学杂志

Stem cells are characterized by the ability to renew themselves and differentiate into a di-verse range of specialized cell types.Bmi1 gene has been determined to play critical roles in the self-renewal of stem cells.Many of the tumor originated in stem cells.Enhanced self-renewal capability in stem/progenitor cells changes its biological characteristic and drives tumor initiation