Interaction between BCSG1 and Hsa-circ-0026352 in genetic susceptibility to invasive breast cancer / 中国肿瘤生物治疗杂志

@#[摘 要] 目的：探讨乳腺癌特异基因1（BCSG1）与Hsa-circ-0026352在浸润性乳腺癌（IBC）遗传易感性中的交互作用。方法：选取2019年6月至2022年5月间武汉市中西医结合医院收治的100例IBC患者作为研究对象，采用免疫组化法检测IBC组织及其相应癌旁组织中BCSG1的表达，将研究对象按照IBC组织中BCSG1蛋白表达的高低分为阴性、弱阳性和强阳性组，统计三组患者的临床病理特征及雌激素受体（ER）、孕激素受体（PR）、表皮生长因子受体-2（HER2）、Hsa-circ-0026352的表达情况，采用Logistic回归方程和最大似然法分析BCSG1表达与上述参数的趋势性和交互作用。结果：与癌旁组织比较，IBC组织中BCSG1蛋白呈高表达（P<0.05）；BCSG1蛋白强阳性表达与淋巴结转移、分化程度、临床分期、HER2表达、 Hsa-circ-0026352表达有关联（P<0.05）；BCSG1强阳性表达与IBC存在交互作用（P<0.05）；BCSG1表达与IBC的交互作用在Hsa-circ-0026352阳性表达中最为显著（趋势P<0.001）；BCSG1表达与IBC的交互作用在临床分期Ⅲ期、低分化程度中最为显著（趋势P<0.001）。结论：BCSG1与IBC患病密切相关，且与Hsa-circ-0026352、临床分期、分化程度存在交互作用，可共同增加IBC患病风险性。

Berberine alleviates myocardial diastolic dysfunction by modulating Drp1-mediated mitochondrial fission and Ca2+ homeostasis in a murine model of HFpEF / 医学前沿

Heart failure with preserved ejection fraction (HFpEF) displays normal or near-normal left ventricular ejection fraction, diastolic dysfunction, cardiac hypertrophy, and poor exercise capacity. Berberine, an isoquinoline alkaloid, possesses cardiovascular benefits. Adult male mice were assigned to chow or high-fat diet with L-NAME ("two-hit" model) for 15 weeks. Diastolic function was assessed using echocardiography and noninvasive Doppler technique. Myocardial morphology, mitochondrial ultrastructure, and cardiomyocyte mechanical properties were evaluated. Proteomics analysis, autophagic flux, and intracellular Ca2+ were also assessed in chow and HFpEF mice. The results show exercise intolerance and cardiac diastolic dysfunction in "two-hit"-induced HFpEF model, in which unfavorable geometric changes such as increased cell size, interstitial fibrosis, and mitochondrial swelling occurred in the myocardium. Diastolic dysfunction was indicated by the elevated E value, mitral E/A ratio, and E/e' ratio, decreased e' value and maximal velocity of re-lengthening (-dL/dt), and prolonged re-lengthening in HFpEF mice. The effects of these processes were alleviated by berberine. Moreover, berberine ameliorated autophagic flux, alleviated Drp1 mitochondrial localization, mitochondrial Ca2+ overload and fragmentation, and promoted intracellular Ca2+ reuptake into sarcoplasmic reticulum by regulating phospholamban and SERCA2a. Finally, berberine alleviated diastolic dysfunction in "two-hit" diet-induced HFpEF model possibly because of the promotion of autophagic flux, inhibition of mitochondrial fragmentation, and cytosolic Ca2+ overload.

Erratum: Author correction to 'Ablation of Akt2 and AMPKα2 rescues high fat diet-induced obesity and hepatic steatosis through Parkin-mediated mitophagy' Acta Pharmaceutica Sinica B 11 (2021) 3508-3526

[This corrects the article DOI: 10.1016/j.apsb.2021.07.006.].

Ablation of Akt2 and AMPKα2 rescues high fat diet-induced obesity and hepatic steatosis through Parkin-mediated mitophagy

@#Given the opposing effects of Akt and AMP-activated protein kinase (AMPK) on metabolic homeostasis, this study examined the effects of deletion of Akt2 and AMPKα2 on fat diet-induced hepatic steatosis. Akt2–Ampkα2 double knockout (DKO) mice were placed on high fat diet for 5 months. Glucose metabolism, energy homeostasis, cardiac function, lipid accumulation, and hepatic steatosis were examined. DKO mice were lean without anthropometric defects. High fat intake led to adiposity and decreased respiratory exchange ratio (RER) in wild-type (WT) mice, which were ablated in DKO but not Akt2−/− and Ampkα2−/− mice. High fat intake increased blood and hepatic triglycerides and cholesterol, promoted hepatic steatosis and injury in WT mice. These effects were eliminated in DKO but not Akt2−/− and Ampkα2−/− mice. Fat diet promoted fat accumulation, and enlarged adipocyte size, the effect was negated in DKO mice. Fat intake elevated fatty acid synthase (FAS), carbohydrate-responsive element-binding protein (CHREBP), sterol regulatory element-binding protein 1 (SREBP1), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), peroxisome proliferator-activated receptor-α (PPARα), PPARγ, stearoyl-CoA desaturase 1 (SCD-1), phosphoenolpyruvate carboxykinase (PEPCK), glucose 6-phosphatase (G6Pase), and diglyceride O-acyltransferase 1 (DGAT1), the effect was absent in DKO but not Akt2−/− and Ampkα2−/− mice. Fat diet dampened mitophagy, promoted inflammation and phosphorylation of forkhead box protein O1 (FoxO1) and AMPKα1 (Ser485), the effects were eradicated by DKO. Deletion of Parkin effectively nullified DKO-induced metabolic benefits against high fat intake. Liver samples from obese humans displayed lowered microtubule-associated proteins 1A/1B light chain 3B (LC3B), Pink1, Parkin, as well as enhanced phosphorylation of Akt, AMPK (Ser485), and FoxO1, which were consolidated by RNA sequencing (RNAseq) and mass spectrometry analyses from rodent and human livers. These data suggest that concurrent deletion of Akt2 and AMPKα2 offers resilience to fat diet-induced obesity and hepatic steatosis, possibly through preservation of Parkin-mediated mitophagy and lipid metabolism.

Internet-plus model for acute stroke service management:exploration and practice / 中华医院管理杂志

In view of the pain points and difficulties in the current stroke service system, the Internet-plus model is merged into the stroke service process for exploration and practice. Mobile Internet technology bridged the process before and after the patient arrives at the hospital, thus extending the green path. This green path is under refined management by mobile applications and 2-dimensional bar code technologies, supporting sustained improvement of data-driven clinical quality. Benefited by"Internet-plus"stroke service management, the efficiency and key performance indicator of the stroke center are significantly elevated.

Analysis on biological characteristics and drug resistance situation of Klebsiella pneumoniae liver abscess / 国际检验医学杂志

Objective To analyzed the clinical characteristics of Klebsiella pneumoniae liver abscess as well as the characteristics and drug resistance situationof Klebsiella pneumoniae .Methods The clinical data in 110 cases of Klebsiella pneumoniae liver ab-scess were collected and divided into the other liver diseases group and non-other liver diseases group for conducting the study .The slime test was adopted to determine the high mucus character of Klebsiella pneumoniae .The PCR amplification was used to detect the virulence genes and capsular type and the drug susceptibility test was performed by using the disk diffusion method .Then the results were statistically analyzed .Results Klebsiella pneumoniae liver abscess was common in 40 -80 years old middle aged and elderly males .50 .00% patients had the diabetes base .In 110 strains of Klebsiella pneumoniae ,86 .36% strains had the high mucus phenotype ,the capsular types were dominated by serotype K1 and K2 .The detection rats of phenotype strains ,carrying virulence gene rmpA and aerocin in the non-other liver disease group were significantly higher than those in other liver diseases group ,the difference was statistically significant (P<0 .01) .The resistance rats of Klebsiella pneumoniae to 12 kinds of common antibacterials were lower .Conclusion Klebsiella pneumoniae liver abscess is common in middle aged and elderly males .High mucus and high vir-ulence Klebsiella pneumoniae carrying virulence gene empA and aerocin is main pathogenic bacterium of liver abscess ,w hich has lower resistance rate to common antibacterials .

Noninvasive monitoring of intra-abdominal pressure by measuring abdominal wall tension / 世界急诊医学杂志（英文）

@#BACKGROUND: Noninvasive monitoring of intra-abdominal pressure (IAP) by measuring abdominal wall tension (AWT) was effective and feasible in previous postmortem and animal studies. This study aimed to investigate the feasibility of the AWT method for noninvasively monitoring IAP in the intensive care unit (ICU). METHODS: In this prospective study, we observed patients with detained urethral catheters in the ICU of Shanghai Tenth People's Hospital between April 2011 and March 2013. The correlation between AWT and urinary bladder pressure (UBP) was analyzed by linear regression analysis. The effects of respiratory and body position on AWT were evaluated using the paired samples t test, whereas the effects of gender and body mass index (BMI) on baseline AWT (IAP<12 mmHg) were assessed using one-way analysis of variance. RESULTS: A total of 51 patients were studied. A significant linear correlation was observed between AWT and UBP (R=0.986, P<0.01); the regression equation was Y=–1.369+9.57X (P<0.01). There were significant differences among the different respiratory phases and body positions (P<0.01). However, gender and BMI had no significant effects on baseline AWT (P=0.457 and 0.313, respectively). CONCLUSIONS: There was a significant linear correlation between AWT and UBP and respiratory phase, whereas body position had significant effects on AWT but gender and BMI did not. Therefore, AWT could serve as a simple, rapid, accurate, and important method to monitor IAP in critical y il patients.

The cultivation and migration in vitro of olfactory ensheathing cells from human olfactory mucosa / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate the bionomics of the olfactory ensheathing cells (OECs) of human olfactory mucosa.@*METHOD@#To separate and cultivate the OECs of human and rat olfactory mucosa. To observe the cell growth, cell grouping and cell migration in vitro of the two types of OECs.@*RESULT@#Successfully separated and cultivated the OECs of human and rat olfactory mucosa. OECs of the human and rat olfactory mucosa had the similar cell growth, cell grouping and cell migration ability in vitro.@*CONCLUSION@#OECs of the human and rat olfactory mucosa have the similar bionomics in vitro, as a result, OECs of the human olfactory mucosa could be a reliable source of cell transplant for nerve injury.

The function and model of sleep K-complex / 第二军医大学学报

K-complex (KC) is a major component of electroencephalography (EEG) in human brain during non-rapid eye movement sleep, and therehas long been a debate on its function. Some researchers consider that KC represents a sign of arousal response of brain to internal or external stimuli, and others suggest that it is a protective response to sustain sleep. In this article we put forward a functional model of KC based on the above two views and related literatures. According to our model, KC arises from three kinds of brain rhythms (sleep cyclic, central oscillation, and cortical oscillation). Based on the results of stimulus significance assessed by sleep-information assessment center, the generation of KC is related to three different responses-total arousal, sign of being awakened, and no sign of being awakened. In conclusion, KC is a reflection of cerebral state responsive to stimuli.

Right ventricular apical versus right ventricular outflow tract pacing: impact on left ventricular synchronization / 南方医科大学学报

<p><b>UNLABELLED</b>Objective To compare the impact of right ventricular apical (RVA) versus right ventricular outflow tract (RVOT) pacing on left ventricular systolic synchronization.</p><p><b>METHODS</b>Sixty patients were prospectively recruited and randomized into RVA group (n=30) with the right ventricle leads placed in the RVA and RVOT group (n=30) with right ventricle leads placed in the septum of the RVOT. Speckle tracking imaging was performed with 100% ventricle pacing to measure the differences in the time to maximum left ventricle (LV) radial strain.</p><p><b>RESULTS</b>In RVA group, the difference in the time to 6-segment maximum LV radial strain after pacing was 105.27 ± 19.74 ms, significantly greater than that in RVOT group (41.65 ± 12.17 ms, P<0.001). The standard difference of time to 6-segment maximum LV radial strain was also significantly greater in RVA group than in RVOT group (42.71 ± 17.63 vs 17.63 ± 5.62 ms, P<0.001).</p><p><b>CONCLUSION</b>Left ventricle systolic synchronizaition after RVOT pacing is superior to RVA pacing.</p>

A clinical study of noninvasive monitoring of intra-abdominal pressure by measurement of abdominal wall tension / 中华急诊医学杂志

Objective To study the practicability of measurement of abdominal wall tension (AWT) for noninvasive monitoring of intra-abdominal pressure in ICU patients.Methods Patients with indwelling urethral catheter admitted to ICU from April 2011 to March 2013 were enrolled for a prospective study.Exclusion criteria were patients with muscular relaxants,abdominal operation in three months,acute peritonitis,abdominal mass,acute injury of urinary bladder,acute cystitis,neurogenic bladder,intrapelvis hematoma,and pelvic fracture.The AWT (N/mm) and urinary bladder pressure (UBP) (mm Hg) of all patients were measured.Statistical analysis was performed by SPSS 13.0 statistical software.The correlation of AWT and UBP were analyzed using linear regression analysis.The effects of respiration and body position on AWT were analyzed using the Paired-samples t test,and the effects of gender and body mass index (BMI) on basic level of AWT (IAP ＜ 12 mm Hg) were analyzed using the One-way ANOVA.Results A total of 51 patients were recruited in study.A significantly linear correlation between AWT and UBP were observed (R =0.986,P ＜ 0.01),the regression equation was Y =1.369 + 9.57X (P ＜ 0.01).Under the supine-flat positioning,the intra-abdominal pressures at the end of inspiration and the end of expiration were (1.65 ± 0.52) N/mm and (1.45 ± 0.54) N/mm,respectively,and under body straight up 30° supine positioning,the intra-abdominal pressures at the end of inspiration and the end of expiration were (1.25 ± 0.30) N/mm and (1.07 ± 0.35) N/mm,respectively.There were significant differences in intra-abdominal pressure between different respiratory phases and body positions (P ＜ 0.01).Basic levels of AWT in male and female were (1.09 ± 0.29) N/mm and (1.01 ± 0.34) N/mm.The basic levels of AWT in patients with BMI ＜ 18.5,18.5 ≤ BMI ≤25 and BMI ＞ 25 were (0.91 ± 0.30) N/mm,(1.02 ±0.35) N/mm and (1.16 ±0.28) N/mm respectively,but gender and BMI had no significant effects on basic level of AWT (P =0.457 and 0.313,respectively).Conclusions There was a significantly linear correlation between AWT and UBP,and respiratory phase and body position had significant effects on AWT,but gender and BMI had no significant impacts on basic level of AWT.AWT could be served as a simple,easy,and accurate method to monitor the IAP in critical ill patients.

Advances in ultrasonic assessment of bone status and osteoporosis / 国际生物医学工程杂志

Research on osteoporosis diagnosis has always been a critical isuue in the field of international academia and medicine. Recent progress in quantitative ultrasound (QUS) has suggested that ultrasound, due to its obvious advantages, be considered as an effective and noninvasive tool for assessment of bone status and diagnosis of osteoporosis. This paper presents the principle and recent development in the ultrasonic assessment of bone status and osteoporosis including assessment with through-transmission and backscattering measurement, and also introduces the latest progress in ultrasonic axial transmission technique.Limitations of current research are discussed and suggestions are proposed for future research.

Study of transdiaphragmatic pressure and its correlation with esophageal pressure in ARDS piglet / 中华急诊医学杂志

Objective To study the changes of trans-diaphragmatic pressure (Ptra) and its correlation with esophageal pressure (Peso) through ARDS piglet model.Methods Five piglets were enrolled in the study.Peso,gastric pressure (Pgas) and intra-thoracic pressure (Pint) was monitored through balloon inserted.The data before ARDS serve as control.ARDS was produced in the piglets through saline lavage.The pressure were observed and the Ptra were calculated.The pressure changes and correlation between Ptra and Peso were analyzed as well.Linear regression with the coefficient of determination and t-test were used as appropriate.Significance was assumed for P ＜ 0.05.Results Peso,Pgas and Pint before ARDS were 7.3 ± 1.9,25.5 ± 2.4,- 1.23 ± 0.21 cmH2O,Ptra was 18.2 ± 1.6 cmH2O.While after ARDS,the data were 4.7 ± 1.4,31.1 ± 3.1 and - 1.79 ± 0.28 cmH2O,and Ptra was 26.4 ± 2.1 cmH2 O,and all these changes were obviously ( P ＜ 0.05 ).The correlation between Pint and Peso,Pint and Ptra (A) and Ptra ( B ) were 0.93 ± 0.025,0.88 ± 0.023 and 0.87 ± 0.37 before ARDS.After ARDS,the correlation changed to be 0.82 ±0.21,0.81 ±0.20 and 0.78 ±0.31.Although a bit decreased,the correlation was still positive (P ＜ 0.01 ).Conclusions There existed good correlations between Peso and Ptra as well as between Pint and Peso before or after ARDS.Ptra was increased obviously after ARDS,which could lead to respiratory muscle fatigue.

Utility of CT Scan in Detection of Melamine - Associated Urinary Stones.

Objective To detail the utility of CT scan in detection of urinary stones induced by melamine tainted formula. Material and Methods A total of 1062 children fed with melamine-contaminated infant formula were screened for urinary stones in our institute from September through December 2008. Ultrasonography of the urinary tract system was performed in all these children. If the children with suspected stones or severe obstruction were presented after ultrasound examination, themulti-detector row CTurographic examination was advocated subsequently. Results Ultrasound examination in combination with multidetector row CT urography could increase the diagnostic rate from 3.4% (36/1062) by ultrasound examination alone to 4.6% (49/1062). Conclusions The specificity and sensitivity of the multidetector row CT urographic examination is higher than ultrasonography.

Improvement on high frame rate ultrasonic imaging system based on linear frequency-modulated signal / 生物医学工程学杂志

The high frame rate (HFR) ultrasonic imaging system based on linear frequency-modulated (LFM) signal constructs images at a high frame rate; the signal-to-noise ratio (SNR) of this system can also be improved. Unfortunately, such pulse compression methods that increase the SNR usually cause range sidelobe artifacts. In an imaging situation, the effects of the sidelobes extending on either side of the compressed pulse will be self-noise along the axial direction and masking of weaker echoes. The improvement on high frame rate ultrasonic imaging system based on LFM signal is considered in this paper. In this proposed scheme, a predistorted LFM signal is used as excited signal and a mismatched filter is applied on receiving end. The results show that the proposed HFR ultrasonic imaging system can achieve higher SNR and the axial resolution is also improved.

Off-line experiments and analysis of independent brain--computer interface / 生物医学工程学杂志

In order to study event-related desynchronization (ERD) related to voluntary movement, we designed two experiments. In the first experiment, untrained subjects were required to imagine the action of typing with left or right index finger for about 1 second before real action, whereas they were required to type instantly after instruction in the second experiment. By analyzing spontaneous EEG signals between the instruction and the action, we predicted which finger was used. The prediction accuracy in the first experiment fell from 85% to 71% with the progress of experiment, the average accuracy being 78%, whereas the prediction result was almost random guess in the second experiment. The results demonstrate that (1) ERD patterns are significantly affected by the effective duration of motion imagination, (2) unconscious reduction of this duration can decrease the prediction accuracy. Therefore, when designing subsequent BCI experiments, we should devote our attention to the question of how to keep the effective duration of motion imagination.

Implementation of sector imaging mode with high signal-to-noise ratio in the high frame rate ultrasonic imaging system / 生物医学工程学杂志

The high frame rate (HFR) ultrasonic imaging system, which is developed with limited diffraction beams, constructs images at a high frame rate. However the rectangular imaging area, to some extent, restricts the far field imaging information. At the same time, because of one transmission for constructing image, the system suffers from low SNR. In this paper we present a computationally efficient method to construct sector mode image and to increase the SNR in HFR system. The method uses Golay complementary sequence as excitation to realize two transmission events. Each event simultaneously transmits two plane waves with different transmission angle. Then the received echo signals related to different angle are separated according to orthogonality of Golay complementary sequence and used to construct two images of different area by HFR method. Finally the two images are synthesized to one frame of sector mode image.

Three-dimensional reconstruction research on Mingmen (GV 4) / 中国针灸

<p><b>OBJECTIVE</b>To show the space structures of Mingmen (GV 4) area and its adjacent tissues.</p><p><b>METHODS</b>A frozen female corpse was cut into about 0.5 mm thick sections with a high-speed steel plane machine and photographed with digital and optical camera. Two 2-D transverse phase picture data sets were obtained and then were sequenced, matched, modified and divided. The 3-D reconstruction software was used to reconstruct the outline form of the lumbar part containing Mingmen (GV 4) to attain transverse phase picture data sets (3 022 sheets, 0.57 mm in thickness). The sagittal, coronal and different oblique plane were cut and the tissue structures in these pictures were analyzed. Thus, the reconstruction data sets were obtained.</p><p><b>CONCLUSION</b>After the structures of Mingmen (GV 4) were reconstructed with computer, the form of Mingmen (GV 4) can be dynamically observed from any angle and the space construction law of gross form of Mingmen (GV 4) can be proved.</p>

Computer simulation of high frame rate ultrasonic imaging system based on the angular spectrum concept / 生物医学工程学杂志

A high frame rate (HFR) ultrasonic imaging method developed by Jianyu Lu has been attracting attention in the related imaging area. Because a special weighting on the received signal is needed to implement this method, the imaging system is complicated. This paper presents a new kind of theroetical analysis for the HFR imaging system in view of the angular spectrum concept. Based on the study, the special weighting process can be replaced by Fourier transform. Computer simulation demonstrates the correctness of the analysis. It shows that the constructed images by the new method have the same high quality as those obtained by using the original HFR method while the system implementation is greatly simplified.

Vector propagation algorithm based ECG simulation of bundle branch block / 生物医学工程学杂志

The simulation of excitation propagation's process in human heart is one of the main aspects of ECG forward problem. The simulation results not only are the criterion of the simulation model's precision and reliability, but also have great value in researches and diagnoses. We performed the simulation of QRST waves of complete left bundle branch block (LBBB) and right bundle branch block (RBBB) in virtue of a vector propagation algorithm (VPA), which is accurate, efficient and applicable to anisotropic computer heart models. The simulation results accord with the actual QRST wave in clinical practice.