Relationship between M2 microphage infiltration and the malignant degree of prostate cancer / 临床与实验病理学杂志

Purpose To investigate the relationship between M2 microphage infiltration and the pathological grading and staging of prostate cancer.Methods 58 patients who accepted the radical resection of prostate cancer were selected.After operation,pathologic specimens were hematoxylin eosin stained and immunohistochemical of SP stained to identify the pathological grading,staging,and M2 microphage infiltration.Then,Chi-squared test was used to analyze the relationship between M2 microphage infiltration and the pathological grading and staging of prostate cancer.Results There were 71.4％ high stage (≥T2c) cases and 60％ lymph node metastasis cases in M2 microphage infiltration group,which was significantly higher than those in non M2 microphage infiltration group.However,there was no relationship between M2 microphage infiltration and pathological grading.Conclusion M2 macrophage infiltration may participate in the prostate cancer cell growth and metastasis,whose pathophysiological mechanism needs further exploration.

Comparison of EGFR mutations in blood and tumor tissue samples by allele-specific PCR / 临床与实验病理学杂志

Purpose To explore the clinical application value of allele specific PCR in detection of ctDNA EGFR mutations by comparing the epidermal growth factor receptor (EGFR)mutation in tumor tissue specimens and circulating tumor DNA (ctDNA) of lung cancer patients by allele-specific PCR.Methods 22 pairs of plasma samples and tumor tissue samples of non-small cell lung cancer (NSCLC) patients were collected and the corresponding cfDNA and tDNA were extracted.The cobas EGFR mutation test v2.0 and cobas EGFR mutation test were used to detected EGFR 18-21 exon mutation,respectively.Results Among 22 patients with NSCLC,there were 8 cases of EGFR mutations in plasma samples,so the mutation rate was 36.4％ (8/22).The presence of EGFR mutations in tumor samples was 13 cases,and the mutation rate was 59.1％ (13/22).EGFR mutation status of 16 patients in plasma samples was consistent with tumor tissues.The result of tDNA gene mutations was regarded as the standard.Sensitivity of gene mutations in ctDNA was 61.5％,and specificity was 100％.The concordance rate between result of tDNA and ctDNA was 72.7％.Conclusion Detection of ctDNA EGFR gene mutations by allele-specific PCR provides a new detection opportunity for patients unable to obtain tumor tissue specimens.

Optimization of subcritical aqueous extraction for polysaccharides from Punica granatum leaves and the in vitro antioxidant activity / 中成药

AIM To optimize the subcritical aqueous extraction for polysaccharides from the leaves of Punica granatum L.and to evaluate the in vitro antioxidant activity.METHODS With reaction pressure,solid-liquid ratio,extraction time and extraction temperature as influencing factors,yield of polysaccharides as an evalution index,the extraction was optimized by Box-Behnken method on the basis of single factor test.Then the scavenging effects of polysaccharides on hydroxyl free radical,superoxide anion and DPPH free radical were detected.RESULTS The optimal conditions were determined to be 5 MPa for reaction pressure,1 ∶ 27 for solid-liquid ratio,11 min for extraction time,and 155 ℃ for extraction temperature,the yield of polysaccharides was 1.809％.There was a dose-effect relationship between scavenging rate and polysaccharides' concentration.0.1 mg/mL Polysaccharides displayed the strongest scavenging effects on hydroxyl free radical,superoxide anion and DPPH free radical with the clearance rates of 57.36％,70.51％ and 58.02％,respectively.CONCLUSION This stable and reliable method can be used for the subcritical aqueous extraction for polysaccharides from P.granatum leaves with obvious in vitro antioxidant activity.

Observation of curative effect of Demolin spray combined with comprehensive nursing on pressure sore / 中国生化药物杂志

Objective To investigate and analyze the clinical efficacy of Demolin spray combined with comprehensive care in the treatment of pressure sores. Methods 56 patients with pressure sores from February 2011 to October 2016 were selected and randomly divided into the control group and the experimental group, with 28 patients in each group. The control group was treated with routine treatment, the experimental group on the basis of the given Demolin spray treatment, and comprehensive nursing, based on fully understanding the clinical data of patients, patients were given comprehensive nursing according to focus on the patient's physical and mental health. The therapeutic effects of the two groups were compared and analyzed. Results After the corresponding treatment, the experimental group treated invalid 4 cases, effective in 8 cases, markedly effective in 10 cases, 6 cases recovered. The number of invalid cases in the control group was 10 cases, effective for 5 cases, markedly effective for 8 cases, recovery for 5 cases. The effective rate of treatment in the experimental group was 85.71 %, which was significantly higher than that in the control group, and the effective rate was 64.28 %, with statistical difference (P<0.05). The cure time of the control group was (13.82 ±3.70) days, and the number of dressing change was (20.40 ± 3.10) times. The cure time and change times of the experimental group were significantly better than those of the control group, the difference was statistically significant (P<0.05). Conclusion Demolin spray combined with comprehensive nursing treatment of bedsore clinical effect is better, can significantly improve the clinical efficacy, with clinical significance.

Optimization of subcritical aqueous extraction for polysaccharides from Punica granatum leaves and the in vitro antioxidant activity / 中成药

AIM To optimize the subcritical aqueous extraction for polysaccharides from the leaves of Punica granatum L.and to evaluate the in vitro antioxidant activity.METHODS With reaction pressure,solid-liquid ratio,extraction time and extraction temperature as influencing factors,yield of polysaccharides as an evalution index,the extraction was optimized by Box-Behnken method on the basis of single factor test.Then the scavenging effects of polysaccharides on hydroxyl free radical,superoxide anion and DPPH free radical were detected.RESULTS The optimal conditions were determined to be 5 MPa for reaction pressure,1 ∶ 27 for solid-liquid ratio,11 min for extraction time,and 155 ℃ for extraction temperature,the yield of polysaccharides was 1.809％.There was a dose-effect relationship between scavenging rate and polysaccharides' concentration.0.1 mg/mL Polysaccharides displayed the strongest scavenging effects on hydroxyl free radical,superoxide anion and DPPH free radical with the clearance rates of 57.36％,70.51％ and 58.02％,respectively.CONCLUSION This stable and reliable method can be used for the subcritical aqueous extraction for polysaccharides from P.granatum leaves with obvious in vitro antioxidant activity.

Observation of curative effect of Demolin spray combined with comprehensive nursing on pressure sore / 中国生化药物杂志

Objective To investigate and analyze the clinical efficacy of Demolin spray combined with comprehensive care in the treatment of pressure sores. Methods 56 patients with pressure sores from February 2011 to October 2016 were selected and randomly divided into the control group and the experimental group, with 28 patients in each group. The control group was treated with routine treatment, the experimental group on the basis of the given Demolin spray treatment, and comprehensive nursing, based on fully understanding the clinical data of patients, patients were given comprehensive nursing according to focus on the patient's physical and mental health. The therapeutic effects of the two groups were compared and analyzed. Results After the corresponding treatment, the experimental group treated invalid 4 cases, effective in 8 cases, markedly effective in 10 cases, 6 cases recovered. The number of invalid cases in the control group was 10 cases, effective for 5 cases, markedly effective for 8 cases, recovery for 5 cases. The effective rate of treatment in the experimental group was 85.71 %, which was significantly higher than that in the control group, and the effective rate was 64.28 %, with statistical difference (P<0.05). The cure time of the control group was (13.82 ±3.70) days, and the number of dressing change was (20.40 ± 3.10) times. The cure time and change times of the experimental group were significantly better than those of the control group, the difference was statistically significant (P<0.05). Conclusion Demolin spray combined with comprehensive nursing treatment of bedsore clinical effect is better, can significantly improve the clinical efficacy, with clinical significance.

Seasonal distribution of active systemic lupus erythematosus and its correlation with meteorological factors

OBJECTIVE: To explore the characteristics of seasonal distribution of active systemic lupus erythematosus (SLE) and the influences of meteorological factors including temperature and humidity on active systemic lupus erythematosus. METHODS: The characteristics of seasonal distribution of active SLE and its correlation with meteorological factors were retrospectively analyzed in 640 patients living in the city of Zhanjiang, China and had active SLE between January 1997 and December 2006. RESULTS: In winter, when there are weaker ultraviolet (UV) rays, the ratio of patients with active SLE to total inpatients was 3.89 percento, which is significantly higher than in other seasons with stronger UV rays, including 2.17 percento in spring, 1.87 0 in summer and 2.12 0 in autumn. The number of patients with active SLE had significant negative correlation with mean temperature and was not significantly related to mean humidity. CONCLUSION: Active SLE has the characteristics of seasonal distribution and is associated with temperature. The mechanism remains to be further studied.

Evaluation of the inhibitory effect of tripeptide tyroservatide on growth of human hepatocellular carcinoma using a hollow fiber assay / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To investigate the inhibitory effect of tyroservatide (YSV) on growth of hepatocellular carcinoma cells.</p><p><b>METHODS</b>In vitro effects of YSV on five human hepatocellular carcinoma cell lines were assayed by MTS. In vivo effects of YSV on 5 human hepatocellular carcinoma cell lines were assayed by hollow fiber tumor model.</p><p><b>RESULTS</b>After treatment with YSV at a dose of 0.1 approximately 1.6 mg/ml, the growth of the five cell lines was significantly inhibited in vitro compared with that of the control group (P < 0.05). Especially, YSL remarkably inhibited the growth of human hepatocellular carcinoma BEL-7402 cells, i.e. the cell growth was inhibited by 63.3% after treatment with YSL at 1.6 mg/ml. The hollow fiber tumor model demonstrated that YSL (320 microg x kg(-1) x d(-1) and 640 microg x kg(-1) x d(-1)) treatment significantly inhibited the in vivo growth of the five cancer cell lines compared with that in the saline control (P < 0.05). YSL showed the highest level of inhibition of human BEL-7402 hepatocellular carcinoma cells, with an inhibitory index of 53.1% at 320 microg x kg(-1) x d(-1).</p><p><b>CONCLUSION</b>As a new method, hollow fiber assay may be used to evaluate the inhibitory effect of drugs on different tumor cells in vivo, rapidly, accurately and economically. Our results provide an instruction and evidence for clinical use of YSV.</p>

Effect of Krüppel-like factor 4 overexpression on heat stress-induced apoptosis of Raw264.7 macrophages / 中南大学学报(医学版)

OBJECTIVE@#To observe the effect of Krüppel-like factor 4 (KLF4) overexpression on heat stress-induced apoptosis of Raw264.7 macrophages.@*METHODS@#The fragment containing full length mouse KLF4 cDNA coding sequence was inserted into the pcDNA3.1 vector and Raw264.7 macrophages were transfected with pcDNA3.1-KLF4 plasmids using lipofectamine.The expression of KLF4 was examined by Western blot in the Raw264.7 macrophages stably transfected with pcDNA3.1- KLF4 plasmids. Raw264.7 cells transfected with pcDNA3.1 and pcDNA3.1-KLF4 were exposed to heat stress (42 degrees C) for 1h and recovered at 37 degrees C for 12h. Flow cytometry, Hoechest 33258 staining assay, and DNA ladder assays were performed to assess the apoptosis.@*RESULTS@#The KLF4 overexpressed Raw264.7 macrophages were established. After the heat stress, flow cytometry showed that apoptotic cells increased significantly in KLF4 overexpressed cells compared with the vector control; Hoechest 33258 staining was characterized with classical changes including apoptotic body, and nuclear condensation. Agarose gel electrophoresis showed that "DNA ladder" could be observed clearly.@*CONCLUSION@#KLF4 overexpression can increase heat stress-induced apoptosis of Raw264.7 macrophages.

Screen of inflammatory genes regulated by heat shock factor 1 and corroboration with SOCS3 gene / 中南大学学报(医学版)

OBJECTIVE@#To screen the inflammatory mediators genes regulated by HSF1, and explore the mechanism of downstream genes regulated by HSF1.@*METHODS@#HSF-/- and HSF1+/+ mice were injected with 15 mg/kg LPS intraperitoneally (ip), respectively, and were treated as previous after HSR. The total RNA of lung tissues were extracted and filtrated by SuperArray gene Microarry. The promoter of candidate genes were analyzed by transcription element search software to search for heat shock element (HSE). Select the suppressor of cytokine signaling 3 (SOCS3) with HSE. Macrophage cells were stimulated with 400 ng/mL LPS, and were treated as previous after HSR, then the total RNA was extracted respectively. RT-PCR and northern blot assay were performed to detect the expression levels of SOCS3 mRNA.@*RESULTS@#Fifteen genes were repressed by HSF1, including 9 genes with complete HSE. Eleven genes were accelerated by HSF1 possibly, including 8 genes with complete HSE. The promoter of SOCS3 gene contained one complete HSE. LPS stimulation obviously increased the levels of SOCS3 mRNA in macrophages of RAW264.7 mice, which was inhibited by HSR and over-expression of HSF1.@*CONCLUSION@#HSR or HSF1 inhibits LPS induced expression of SOCS3 mRNA; HSF1 might inhibit LPS-induced expression of SOCS3 mRNA by binding to HSE in the promoter of SOCS3 gene.

Immortalization of embryonic fibroblasts in heat shock transcription factor 1 knockout mouse / 中南大学学报(医学版)

OBJECTIVE@#To establish immortalized embryonic fibroblast lines in heat shock transcription factor 1 (HSF1) HSF1-/- and HSF1+/+ mice and to provide experimental models to study the function of HSF1.@*METHODS@#A mammalian expression vector (pSV3neo) containing the SV40 large T antigen was used to transfect the HSF1-/- and HSF1+/+ mouse embryonic fibroblast using Lipofectamine 2000. Colonies were screened by G418 and expanded to immortalized cell lines. PCR was used to detect the integration of the large T antigen with genome in the mouse embryonic fibroblast. Expression of SV40 large T antigen gene in expanded cells was identified by RT-PCR. HSP70 expression was examined by Western blot in the embryonic fibroblast lines.@*RESULTS@#The stable growth and serial propagation were observed in the HSF1-/- and HSF1+/+ cell lines for six months. The mRNA of SV40 T antigen gene expressed in the two cell lines. HSP70 expression could not be induced in the heat-treated HSF1-/- mouse embryo fibroblasts.@*CONCLUSION@#The immortalized cells of HSF1+/+ and HSF1-/- mouse embryo fibroblasts are successfully established.

CoCl2-induced chemotherapy resistance in SW480 cells and its mechanism / 中南大学学报(医学版)

OBJECTIVE@#To observe the proliferation of SW480 cells exposed to different concentrations of CoCl2, and to examine the expression of hypoxiainducible factor-1 alpha (HIF-1alpha) and heme oxygenase-1 (HO-1) during hypoxia to explore the chemotherapy resistance effect and role of HIF-1alpha and HO-1.@*METHODS@#Methyl thiazolyl tetrazolium (MTF) method was used to detect the proliferation of SW480 cells in the presence of fluorouracil (FU). RT-PCR was applied to examine the expression of HIF-1alpha and HO-1 mRNA in hypoxia.@*RESULTS@#SW480 cells were proliferated at a slow rate, and had a strong resistance to FU with the increase of CoCl2. RT-PCR showed that the up-regulated expression of HIF-1alpha and HO-1 mRNA was consistent with the dose-effect curve and time-effect curve.@*CONCLUSION@#The hypoxia induced by CoCl2 can inhibit the proliferation of SW480, and it can also decrease the sensitivity of the cell to FU. The mechanism is probably related to the up-regulated expression of HIF-1alpha and HO-1 mRNA.

Characteristics and expression of Mip5, a novel gene associated with myocardial ischemia/reperfusion in rats / 中南大学学报(医学版)

OBJECTIVE@#To determine the characteristics of a novel gene Mip5 (GenBank accession number AY553870) and its expression under physiological and pathological conditions.@*METHODS@#The characteristics of Mip5 were analyzed by bioinformatic programs including BLAST, spidey, psort, ClustalW and so on. RT-PCR was performed to detect Mip5 expression.@*RESULTS@#Bioinformatic analysis showed that Mip5 gene lied in the 13th chromosome and contained 8 exons and 7 introns, its open reading frame contained 909 bp and its protein production was 302 amino acid residues including 6 kelth domains. Under normal conditions, MIP5 expressed abundantly in the heart, brain and kidney, but its expression could not be detected in the liver and muscle. Expression of Mip5 gene was increased significantly after ischemia-reperfusion compared with the sham groups, and reached its peak at 3 h and recovered at 12 h after the reperfusion. Conclusion Mip5 gene is a novel gene containing a putative open reading frame of 302 amino acids residues and may play an important role in rat cardiomyocytes suffering ischemia processing.

Efficacy of Lamivudine Combined with Thymosin ?_1 on Young Patients with Chronic Hepatitis B / 实用儿科临床杂志

Objective To investigate the efficacy of lamivudine( LAM) combined with thymosin ?1 (T?1) on young patients with chronic hepatitis B. Methods Sixty six chronic hepatitis B (CHB) patients aged from 12 to 16 years and weight greater than 33 kg were divided into the LAM + T?1 group and the LAM group; in LAM + T?1 group,T?1 was administered 1.6 mg hypodermic once daily and after 5 days it was changed to 1 6 mg hypodermic, twice in two weeks, and the drug was stopped after 26 weeks, while LAM had been administered 100 mg each time once daily for 52 weeks. In LAM group, only LAM had been administered 100 mg once daily for 52 weeks. The serum level of HBV DNA, HBV markers and the ALT were measured before and after treatment Results After the treatment, the rate of serum HBV UNA turned to negative in LAM + T?1 group was 100 % , and that in LAM group was 93.7 % ; recovery ratios at 52 weeks of LAM+ T?1 group was 87.1% ,and that of LAM group was 78. 1% .After 52 weeks, the HBeAg serum conversion rate of LAM + T?1 group was 58. 1 % , and that of the LAM group was 21.9%. Comparing the results of the two groups, the difference was remarkable. Conclusion The efficacy of T?1 combined with LAM on young patients with chronic hepatitis B is superior to that use of single LAM