Intrauterine injection of human chorionic gonadotropin improves pregnancy outcome in patients with repeated implantation failure in frozen-thawed embryo transfer / 中南大学学报(医学版)

To investigate whether intrauterine injection of human chorionic gonadotropin (hCG) before the embryo transfer in a frozen-thawed transfer cycle can improve the pregnancy outcome in the patients with repeated implantation failure (RIF).
 Methods: Prospective randomized-controlled trial was adopted. A total of 140 patients, who underwent thawed embryo transplantation and were in line with the diagnosis of RIF, were included. Other patients with some factors, such as uterine malformation, postoperative uterine cavity sticking, tubal effusion, endocrine diseases and endometriosis, were excluded. The patients were randomly divided into 2 groups through the computer random number table: an hCG intrauterine perfusion group and a control group. There was no significant difference in the age, the estradiol level, the number of transplanted embryos, the number of optimal embryos, and the thickness of the endometrium before transplantation between the 2 group (all P>0.05). The hCG+G2 fluid and the G2 fluid were prepared on the day of embryo transfer, and 40 μL of which was injected at an intrauterine site at 3 minutes before embryo transfer in the hCG intrauterine perfusion group and the control group, respectively. The clinical pregnancy rate and implantation rate in the 2 groups were compared.
 Results: The implantation rate and the clinical pregnancy rate in the hCG intrauterine perfusion group were higher than those in the control group (both P<0.05).
 Conclusion: The intrauterine injection of hCG can improve the implantation rate and pregnancy rate in cryopreserved embryo transfer in patients with RIF.

In vitro maturation,fertilization and embryo transfer of immature oocytes derived from nature cycles / 重庆医科大学学报

Objective:To investigate the effect of in vitro maturation(IVM),fertilization and embryo transfer of immature oocytes derived from nature cycles in infertility couples.Methods:30 IVF/ICSI-ET cycles were involved in this study.Oocytes retrieval performed after follicle larger than 10 mm and before endogenesis Luteinizing hormone(LH) surge occurrence in nature cycle.Mature oocytes(Metaphase Ⅱ) were inseminated by standard in vitro fertilization(IVF) on the oocytes pick-up day.Immature oocytes(Metaphase I and germinal vesicle stage) were cultured for 24~48 hours and fertilized by intracytoplasmic sperm injection(ICSI) as soon as it developed to Metaphase Ⅱ stage.Embryo transfer was performed 72 h after fertilization.Results:2 of 30 IVM cycles brought no oocytes in this study.113 oocytes were obtained among other 28 oocyte retrieval cycles,74 of which were immature,29 were mature,and 10 were degenerated.60 of 74 immature oocytes developed to MⅡ after IVM(maturation rate 81.08%).6 cases of clinical pregnancy were obtained in the 20 embryo transfer cycles,five of which brought healthy babies(one came from immature oocyte and four came from mature oocyte) and one was abortive.Conclusion:IVM,fertilization and embryo transfer of immature oocytes derived from nature cycles is a feasible treatment for women with infertility.