Clinical analysis of diabetes complicated with mandibular osteomyelitis / 实用口腔医学杂志

The clinical features and treatment of 21 cases of diabetes complicated with mandibular osteomyelitis in Nnazi Mmoja hospital from 201 1 to 201 3 were retrospectively analyzed.After treatment by antibiotics,control of blood sugar and sequestrum scraping surgery,the normal values of ESR,CRP and blood routine were achieved from abnormal in 1 6 /1 8,1 3 /1 5 and 1 2 /1 4 of the patients.86% of the patients (1 8 /21 )obtained clinical cure,while 3 patients showed recurrence within 3 to 24 months.

Construction of the siRNA expression vector and the silencing influence on focal adhesion kinase gene of Tca8113 cell line / 实用口腔医学杂志

Objective:To construct the siRNA expression vector of focal adhesion kinase(FAK) gene and inhibit the expression of FAK gene in tongue cancer cell line Tca8113 by RNA interfering technique. Methods:According to the encoding sequence of FAK mRNA, two pieces of oligonucleotide sequences were designed and synthesized. The annealed oligonucleotide fragments were subcloned into pGCSilencerTM-U6/Neo siRNA expression vector. After being identified by restriction enzyme method, the recombinant pSilencer-FAK plasmids were transfected into Tca8113 cells. The transfected cells were selected by G418 method. Immuocytochemistry and Western blotting were used to evaluate FAK gene silencing efficiency. Results:The oligonucleotide fragments were correctly inserted into pGCSilencerTM-U6/Neo vector. FAK expression of the transfected cells was significantly down-regulated by pSilencer-FAK. Conclusion:The siRNA expression vector of FAK is successfully constructed and FAK expression of Tca8113 cells can be inhibited by RNA interfering technique.