Preliminary Study on Serum Metabolites of Primary Biliary Cholangitis by Hydrogen Spectrum Nuclear Magnetic Resonance / 胃肠病学

Hydrogen spectrum nuclear magnetic resonance (1 H-NMR ) is a commonly used method for metabolomics and has been applied in the study of liver cirrhosis and liver cancer,however,study on serum metabolites in patients with primary biliary cholangitis (PBC)is rare.Aims:To investigate the capability of 1 H-NMR for screening serum metabolites of PBC.Methods:Twenty PBC patients,20 HBV-related cirrhosis patients and 20 healthy controls were detected by 1 H-NMR.The 1 H-NMR spectra data were processed by principal component analysis (PCA)and orthogonal partial least squares-discriminant analysis (OPLS-DA)so as to create the diagnostic model.Based on the correlation coefficient P (corr),VIP value and non-paired t test of OPLS-DA model,the differential metabolites between normal group and the two cirrhosis groups were screened.Results:OPLS-DA model could effectively distinguish healthy controls and PBC patients,model interpretation ability and prediction ability were 81.9% and 44.8%,respectively (P=0.0293), glutamine,folic acid,urocanic acid,4-ethylbenzoic acid were differential metabolites.OPLS-DA model could also effectively distinguish healthy controls and HBV-related cirrhosis patients, urocanic acid, 1-methylhistamine, 1-methyladenine,glucose,L-acetylcarnitine were differential metabolites.OPLS-DA model could not effectively distinguish PBC patients and HBV-related cirrhosis patients.Conclusions:Serum glutamine and folic acid may be the potential biomarkers of PBC,which may be closely related to the immune damage mechanism and prognosis of PBC;1 H-NMR combined with OPLS-DA diagnostic model are expected to become a new method for studying liver cirrhosis.

Female mycoplasma urogenital infections and drug-resistance / 国际检验医学杂志

Objective To investigate the female mycoplasma urogenital infections and drug-resistance.Methods Liqid medium was used to indentify mycoplasmas and detect the susceptibility in vitro of thirteen antimicrobial agents.Results The total rate of mycoplasma infection was 44.0%, including Uu 37.1%, Mh 0.9%, Uu and Mh compound infection was 6.0%. The susceptible rate of more than 80% were jossamycin (90.8%), clarithromycin (88.5%),azithromycin (84.8%) and minocycline (81.2%). The susceptibilities in vitro of all sorts of antibiotic decreased significantly for Uu and Mh compound infections.Conclusion Mycoplasmas produce particular resistance to antibiotics.Jossamycin,clarithromycin,azithromycin and minocycline can be the drugs of first choice.