The cultivation and migration in vitro of olfactory ensheathing cells from human olfactory mucosa / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate the bionomics of the olfactory ensheathing cells (OECs) of human olfactory mucosa.@*METHOD@#To separate and cultivate the OECs of human and rat olfactory mucosa. To observe the cell growth, cell grouping and cell migration in vitro of the two types of OECs.@*RESULT@#Successfully separated and cultivated the OECs of human and rat olfactory mucosa. OECs of the human and rat olfactory mucosa had the similar cell growth, cell grouping and cell migration ability in vitro.@*CONCLUSION@#OECs of the human and rat olfactory mucosa have the similar bionomics in vitro, as a result, OECs of the human olfactory mucosa could be a reliable source of cell transplant for nerve injury.

Applied anatomy of intercavernous sinuses for transsphenoidal endoscopic pituitary surgery / 第二军医大学学报

Objective:To search for a safe approach for transsphenoidal endoscopic pituitary surgery,so as to avoid injury of intercavernous sinuses during the operation.Methods: The anterior intercavernous sinus,posterior intercavernous sinus,inferior intercavernous sinus,basilar sinus,and dorsum sella sinus of 20 Chinese adult cadavers were surgically observed;and the relevant data were obtained and analyzed.Results: The existence rates of anterior intercavernous sinuses,posterior intercavernous sinuses,inferior intercavernous sinuses,basilar sinuses,dorsum sella sinuses were 95%(19),75%(15),10%(2),100%(20) and 30%(6),respectively;their anteroposterior diameters were(2.08?0.90) mm,(5.14?2.54) mm,(1.30?0.40) mm,((2.26?)1.02) mm,and(2.01?0.80) mm,respectively;and their supra-inferior diameters were(2.74?0.96) mm,((1.10?0.74) mm,)(1.48?0.29) mm,(15.67?4.54) mm,and(3.35?1.93) mm,respectively.The distance between inferior edge of anterior intercavernous sinus and the anterior edge of posterior intercavernous sinus was(5.78?1.89) mm.(Conclusion:)The intercavernous sinus should be avoided to expose the pituitary after opening the antapex of sella trucica during transsphenoidal endoscopic pituitary surgery.When the intercavernous sinus can not be avoided,reasonable incision and hemostatic method should be selected before operation to prevent severe bleeding.

Applied anatomy of anterior wall of sphenoid sinus for transsphenoidal endoscopic sella surgery / 第二军医大学学报

Objective:To provide anatomic data for accurately localizing aperture of sphenoidal sinus without injurying sphenopalatine arteries in endoscopic transsphenoidal sella surgery.Methods: The anterior walls of spheroid sinus were observed and measured(layout,position,external diameter,distances to other structures of interest) with a vernier caliper and an angle gauge on 15 adult cadavers.Results: The distances from the root of columella nasi to the inforior pole of sphenoidal sinus aperture and the sphenopalatine foramen were(60.40?3.21,58.10-72.76) mm and(62.14?1.93,59.50-73.40) mm,respectively.The distance from the inforior pole sphenoidal sinus aperture to the sphenopalatine foramen was(12.20?1.10,8.10-16.35) mm.The diameter of the sphenopalatine artery was(1.99?0.13,1.50-2.80) mm.The distances from the inforior pole of sphenoidal sinus aperture to the superior and inferior posterior artery of nasal septum were(3.49?0.24,2.78-5.20) mm and(6.42?1.08,(4.30-8.50)) mm,respectively.Conclusion: Aperture of sphenoidal sinus is an important marker in endosopic transsphenoidal sella surgery.The anterior wall of sphenoid sinus should be opened from the inforior pole of sphenoidal sinus aperture to avoid injurying sphenopalatine artery.