RESUMO
Objective To observe the effect of endothelin-1 (ET-1) on the cell growth, adhesion, migration and expression of intercellular adhesion molecule 1 (ICAM-1) by A375 human malignant melanoma cells. Methods A375 cells were cultured in the presence of ET-1 of various concentrations (0.002, 0.02, 0.2, 2 μg/mL) for different periods. MTT method and flow cytometry were applied to detect the proliferation and ICAM-1 expression of these cells, respectively, after 24-, 48-, and 72-hour treatment. After 24-hour treatment, the cell adhesion and migration of A375 cells were assessed with cell adhesion assay and Transwell chambers, respectively. Results In the case of ET-1 from 0.002 to 0.2 μg/mL, it enhanced the proliferation, adhesion, migration of A375 cells and inhibited the expression of ICAM-1 by A375 cells in a dose dependent manner (P<0.01 or<0.05); however, for ET-1 of 2 μg/mL, the situation was the opposite. Moreover, after 24-hour culture with ET-1 of 0.2 μg/mL, the metabolic activity, cell adhesion rate, and expression of ICAM-1 peaked at 0.327±0.009, (163.31±4.05)% and 4.667±0.551, respectively. Conclusion ET-1 may enhance cellular metabolism and pigmentation by suppressing the expression of ICAM-1 and promoting the proliferation, adhesion and migration of melanoma cells.