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Objective:To evaluate the effect of propofol on proliferation, invasion and migration of human melanoma cells and role of cyclooxygenase-2 (COX-2)/prostaglandin E2 (PGE2)/matrix metalloproteinase (MMP) signaling pathway.Methods:SKMEL-5 cells were cultured in vitro and divided into 4 groups ( n=36 each) using the random number table method: control group (group C), propofol group (group P), COX-2 overexpression group (group COX-2), and COX-2 overexpression plus propofol group (group COX-2+ P). Propofol at the final concentration of 60 μmol/L was added in group P. The COX-2 overexpression plasmid pcDNA3.1-COX-2 was transfected into SKMEL-5 cells in group COX-2 and group COX-2+ P, and propofol at the final concentration of 60 μmol/L was added in group COX-2+ P.After incubation for 48 h, the cell proliferation rate was determined by CCK-8 method, the cell invasion and migration ability was determined by Transwell assay, the expression of COX-2 in cells was detected by Western blot, the expression of COX-2 mRNA in cells was detected by quantitative real-time polymerase chain reaction, and the concentrations of serum PGE2, MMP-2 and MMP-9 were determined by enzyme-linked immunosorbent assay. Results:Compared with group C, the cell proliferation rate was significantly decreased, the number of cell invasion and migration was decreased, the expression of COX-2 protein and mRNA was down-regulated, and the concentrations of PGE2, MMP-2 and MMP-9 in the supernatant were decreased in group P, and the cell proliferation rate was significantly increased, and the number of cell invasion and migration was increased, the expression of COX-2 protein and mRNA was up-regulated, and the concentrations of PGE2, MMP-2 and MMP-9 in the supernatant were increased in group COX-2 ( P<0.05). Compared with group P, the cell proliferation rate was significantly increased, and the number of cell invasion and migration was increased, the expression of COX-2 protein and mRNA was up-regulated, and the concentrations of PGE2, MMP-2 and MMP-9 in the supernatant were increased in group COX-2+ P ( P<0.05). Conclusions:Propofol can inhibit the proliferation, invasion and migration of human melanoma cells, and the mechanism may be related to inhibition of the COX-2/PGE2/MMP signaling pathway.
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Objective To evaluate the effect of thoracic paravertebral block (TPVB) on inflammatory responses in the patients undergoing thoracoscopic radical resection of lung cancer with general anesthesia.Methods Eighty patients of both sexes,aged 18-64 yr,with body mass index of 18-24 kg/m2,of American Society of Anesthesiologists physical status Ⅰ or Ⅱ,scheduled for elective thoracoscopic radical resection of lung cancer,were divided into 2 groups (n =40 each) using a random number table method:control group (group C) and TPVB group.Ultrasound-guided TPVB was performed with 0.5％ ropivacaine 10 ml which was injected into the paravertebral space of T4,7 at 30 min before anesthesia induction in group T.Patient-controlled intravenous analgesia (PCIA) was performed at the end of surgery.When visual analogue scale score＞3,morphine 5-10 mg was intravenously injected.Venous blood samples were collected immediately before surgery (T0) and at 12,24 and 48 h after surgery (T1-3) for determination of serum interleukin-6 (IL-6) and IL-10 concentrations by enzyme-linked immunosorbent assay.The pressing times of PCIA and total volume of fluid infused were recorded at T2,3.The patients were followed up for 48 h after surgery,the development of nausea and vomiting,somnolence and pruritus was recorded,and the total occurrence of adverse reactions was calculated.Results Compared with group C,the pressing times of PCIA and total volume of fluid infused were significantly decreased at T2,3,the incidence of nausea and vomiting,total incidence of adverse reactions and the number of cases required morphine were decreased,and the serum concentration of IL-6 was decreased and the serum concentration of IL-10 was increased at T1-3 in group T (P＜0.05).Conclusion TPVB can alleviate inflammatory responses in the patients undergoing thoracoscopic radical resection of lung cancer with general anesthesia.
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Objective To study the clinical efficacy of Salvia Miltiorrhiza Injection on prevention splenectomy surgery of deep vein thrombosis (DVT). Methods 132 patients who would received splenectomy were selected as an object of study from June 2011 to June 2013. They were divided into the treatment group and the control group by randomizing. The two groups were surgery treated with the same methods and other postoperative. The treatment group received intravenous drip of Salvia Miltiorrhiza Injection after 6 hours once daily for 10 consecutive days. The control group received subcutaneous low molecular weight heparin like the time of the treatment group. Results There was a difference in the incidence of DVT between the two groups (P < 0.05). Conclusion It is effective that Salvia Miltiorrhiza injection in preventing deep vein thrombosis after splenectomy.
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0.05),that of AR 84.21% and 50.00% (P0.05). Conclusions: The pathogenesis of SS salivary glands appears to be linked to decrease or loss of topical action of androgens.Sex hormones act on epithelial cells of salivary glands through corresponding receptors.
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Objective To develop a method for determination of trace zinc in human hair. Methods ?-cyclodextrin polymer(?-CDP) was synthesized using epichlorohydrin as the cross-link agent and it was used as the carrier of 2-?5-bromo-(2-pyridylazo)-5-diethylaminophenol. Resin-phase spectrophotometric method with (?-CDP)and 5-Br-PADAP was used for determination of trace zinc. Results The maximum absorbance of inclusion resin was at 570 nm with apparent molar absorption of 8.63?105 L/(mol?cm). The Beer's law was obeyed in the range of 4-720 ?g/L. The regression equation of calibration was y=0.001 1+0.132 0x, with a correlation coefficient of 0.998 4 and the recovery rates were 99.5%-100.4%. Conclusion The method is simple, sensitive, accurate and can be used for the determination of trace zinc in human hair with satisfactory results.