Effects of Zuogui Pill on osteoclast activity and expression of miR-133b-3p/RhoA in postmenopausal osteoporosis rats / 国际中医中药杂志

Objective:To study the effects of Zuogui Pills on the expressions of miR-133b-3p and RhoA in osteoclasts of postmenopausal osteoporosis rats; To discuss its potential mechanism.Methods:SD female rats were randomly divided into normal group, model group, sham-operation group, and Zuogui Pills group using a random number table method, with 6 rats in each group. The model group and Zuogui Pills group were treated with oophorectomy to construct a rat model of osteoporosis. Zuogui Pills group was orally administered with Zuogui Pills decoction at a concentration of 10 g/kg for 12 consecutive weeks. Colorimetric method was used to measure the serum calcium and phosphorus levels of rats, and ELISA method was used to detect ALP levels. Bone density meter was used to measure the bone density of the femurs of rats in each group. The osteoclast of each group were cultured, and the expressions of RANKL and RUNX2 protein were detected by Western blot. MiRNA sequencing and differential expression analysis were performed on bone tissues of rats. Osteoclasts were treated with miR-133b-3p mimic and its negative control. The cell proliferation activity of osteoclasts was detected by cell counting kit-8 (CCK-8). The osteoclast differentiation activity was detected by the tartrate-resistant acid phosphatase staining. The dual-luciferase reporter assay was used to detect the relationship between miR-133b-3p and RhoA. The "rescue" experiment of miR-133b-3p mimic and RhoA co-expression were used to study the molecular regulatory mechanism of Zuogui Pills on osteoclast activity.Results:Compared with the model group, the bone mineral density of Zuogui Pills group significantly increased ( P<0.05, P<0.01), the levels of calcium and phosphorus in serum increased, the level of alkaline phosphatase ALP decreased ( P<0.05), the expression of RANKL protein decreased, and the expression of RUNX2 protein increased. Sequencing results showed that rno-miR-133b-3p was down-regulated in osteoclasts of postmenopausa osteoporosis rats treated with Zuogui Pills with the maximum difference ( P<0.01). Q-PCR results showed that the expression of miR-133b-3p in osteoclasts of Zuogui Pills group was significantly lower than that of the model group. The upregulation of miR-133b-3p could significantly promote the cell proliferation and differentiation of osteoclasts. RhoA overexpression could reverse the excessive proliferation and differentiation of osteoclasts caused by miR-133b-3p overexpression. Conclusions:RhoA is the target gene regulated by miR-133b-3p. Zuogui Pills can inhibit the activity of osteoclasts by regulating miR-133b-3p/RhoA axis, relieving the symptoms of osteoporosis.

The reference range of copper,iron,zinc,calcium and magnesium in peripheral blood of preschool children in Suzhou / 国际检验医学杂志

Objective Accodring to the guideline of Reference Intervals in the Clinical Laboratory(ISO15189) ,to establish the reference range of Cu ,Fe ,Zn ,Ca and Mg in the peripheral blood of preschool children in Suzhou .Methods Using atomic absorp-tion spectrometry to detect the levels of Cu ,Fe ,Zn ,Ca and Mg in peripheral blood of 3 992 Kindergarden children in Suzhou from Apr to May 2014 ,according the age ,the children were divided into three groups ,1 511 cases in 3 to 4 years old group including 750 boys ,761 girls ,1 169 cases in 4 to 5 years old group including 570 boys ,599 girls ,1 312 cases in 5 to 6 years old group inclu-ding 604 boys 708 girls .According to NCCLS's guideline C28 -A2 to define the reference range .Results The concentration of copper ,iron ,zinc ,calcium ,magnesium had no significant differences in different gender and age groups(P>0 .05) ,the reference ranges in peripheral blood of the preschool children in Suzhou were Cu 11 .47-32 .53 umol/L ,Fe 6 .86-9 .80 mmol/L ,Zn 64 .46-133 .02 umol/L ,Ca 1 .47-2 .09 mmol/L ,Mg 1 .21 -1 .75 mmol/L .Conclusion There are regional difference on the levels of Cu ,Fe ,Zn ,Ca ,Mg in children's peripheral blood ,each region should to establish the local reference range follow the guideline .

Some Important Aspects in Medical Education / 中华医学教育探索杂志

Nowadays,in order to cultivate a great number of high-quality clinicians,on students it is essential for medical university to strengthen education of humanities,excite aspiration for innovating,cultivate innovation ability and teach the modern medical knowledge with science and rational method.The author has given his reasons and made suggestions for the opinion in the article.

Long-term Renal Allograft Dysfunction Associated with Cytomegalovirus Infection / 中国医师杂志

Objective To investigate whether cytomegalovirus (CMV) infection is associated with chronic allograft nephropathy (CAN). Methods 131 patients underwent kidney transplantation from August 1, 1999 to November 30, 2000 were enrolled in this study. CMV-pp65 antigen load (the number of CMV-pp65-positive leukocytes) in peripheral blood within 6 months of posttransplant was detected, and TGF-? 1 mRNA expression in renal allograft biopsy was measured at the 7th month after transplant. The renal functions of the patients were followed up at least three years. Results The TGF-? 1 mRNA level in allografts of the patients with long-time and high-level CMV-pp65 antigen load was obviously higher that that of the patients without. 3 years after transplant, there were lower Ccr and more renal dysfunction in the patients with long-time and high-level CMV-pp65 antigen load than in the patients without, the difference of which was significant between the two groups of patients. Conclusion Serious CMV infection with long duration can damage the function of kidney graft, and is a risk factor for renal dysfunction.

Protective Effect of Alprostadil on Renal Graft Functions in Early Period / 中国药房

OBJECTIVE:To assess whether Alprostadil plays a role in improving renal function of renal recipi?ents.METHODS:A randomized control clinical trial was designed between January1,2001and February28,2004.Alprostadil was administered in85renal recipients who received60?g Alprostadil while transplanting kidney and each day after opera?tion.The effects of Alprostadil were compared with the control group which included276recipients to determine the influences of Alprostadil on urine,creatinine(Cr)and creatinine clearance(Ccr).Under Doppler Ultrasound the renal blood flow resis?tance_indexes(RI)were measured.The rates of acute renal graft rejection(AR)and delayed graft function(DGF)were also calculated in both groups.RESULTS:Urine and Ccr were significantly higher in Alprostadil_treated group than in control.On the contrary Cr and RI were significantly lower in Alprostadil_treated group than in control.Alprostadil_treated group also showed a significantly lower incidence of DGF,but the incidences of rejection in both groups were equal.CONCLUSION:The findings suggest that the addition of Alprostadil to renal recipients improves early graft function and reduce the incidence of DGF,but does not influence the incidence of rejection.

A clinical investigation for relation between urine TGF-?_1 and long-term function of renal allograft / 解放军医学杂志

Objective To explore the relationship between urine TGF-? 1 concentration and long-term function of renal allograft. Methods From August 1, 1999 to December 30, 2000, the urinary content of TGF-? 1 was determined in 146 patients who had had renal transplantation one year ago but with normal renal function. The relative content of urine TGF-? 1 was 172.5～533.1pg/mg Cr. Forty patients each with higher or lower urine TGF-? 1 concentrations were selected to form group Ⅰ and Ⅱ. Two years later, renal function variables, blood and urine TGF-? 1 concentrations were compared between the two groups. The relation between urine TGF-? 1 and chronic allograft nephropathy (CAN)was assessed. Biopsies of transplanted kidneys were carried out in recipients whose creatinine was higher than normal. Results Three years after transplantation, creatinine clearance rate was lowered by 12.8?10.6ml/min in group Ⅰ patients, and 29.6% of them were diagnosed as CAN. Compared with group Ⅱ, the lowering of creatinine clearance rate was more intense and the incidence of CAN was higher, with obviously higher urinary TGF-? 1 content. The differences were significant. Conclusions TGF-? 1 may play a role in the pathogenesis of CAN after renal transplantation, and urinary TGF-? 1 may be considered as an indicator in assessing long term renal function in such case.