Herbal activation of mammary gland; a comparative morphometrical and biochemical analysis

Seventy five adult virgin female Norway rats [60 experimental and 15 controls] were used to evaluate the effect of seeds of three herbs [Fennel, Cumin and Garden cress] on their mammary glands. Experimental animals were fed with these herbs [each type of herb seeds was given to twenty experimental rats] for fourteen days. Rats were sacrificed and mammary gland sections were obtained, stained then morphometrically assessed. Serum prolactin level was performed too. Results revealed that Garden cress seeds are the strongest lactogenic agent among the three. Both Fennel and Cumin seeds were shown to be very weak galactagogues

Subcutaneous pancreatic transplantation shows success in the treatment of experimentally induced diabetes mellitus in golden hamsters

To evaluate subcutaneous pancreatic transplantation in an experimentally induced diabetes mellitus in golden hamsters. Many indexes [histological /using two stains, histochemical /using three enzymes, morphometrical and biochemical /blood glucose level] were employed in this assessment. Alloxan was used as beta-cytotoxic therapy [to induce diabetes mellitus], and cyclophosphamide was employed as immunosuppressive agent. High rate of success of transplantation [clearly evident by microscopical features of viable endocrine and exocrine tissue of transplanted pancreas and great tissue vascularization, normal enzymatic activities and significant lowering of blood glucose level] were elicited. Subcutaneous pancreatic transplantation should be thank about in treatment of diabetes mellitus

Garden cress seed could be a factual galactagogue

Trivial number of books, concerning traditional medicine, had mentioned a galactagogual role of Garden cress seeds. Others ignore that. This controversy, in addition to the steroid [family of sex hormones] contents of the seeds, directed us to evaluate the role of this herb in mammogenesis and lactogenesis. Twelve parameters were used to assess the effect of Garden cress seeds on the mammary gland of young adult virgin rats. These parameters comprise gross assessment, histological examination [routine/ haematoxylin and eosin stain and special stain/ PAS], enzymatic histochemical study [alkaline phosphatase, acid phosphatase and lipoprotein lipase cytochemical localization], biochemical estimations [hormonal assay of FSH, LH, prolactin, estrogen and progesterone] and morphometrical measurements. All parameters significantly exhibited a strong mammotrophic and lactogenic effects of Garden cress seeds on the non-primed mammary gland of adult virgin rat. Garden cress seeds are most probably a real galactagogue and might be useful in induction of lactation

Testing bromocriptine dose necessary for suppression of lactation in rats: morphological study

The lowest dose of bromocriptine, necessary for suppression of lactation in rats, was estimated in this investigation. Fifty healthy lactating rats were treated with different doses of bromocriptine. Cessation of lactation was assessed clinically and histologically. Revealed that the lowest dose capable of lactation suppression is 4 mg bromocriptine / kg body wt. / day. It is very important to know the exact dose, which can suppress lactation in rats because these laboratory animals are commonly employed in experiments concerning this topic

Does a new pregnancy interfere with an already established location in rats

Two parameters, mammary gland morphology and liters' weekly weight gain were involved in this study, to assess a superimposed pregnancy on lactating female. Thirty five, female, Norway, albino rats [Rattus norvegicus] 7-10 days lactating were mated with males. When a new pregnancy occurred, litters of the pregnant-lactating rats were weekly weight. Two weeks after pregnancy, the pregnant-lactating rats were sacrificed, and their mammary glands histology were studied. Simultaneously, other five lactating and five pregnant rats were sacrificed. These two groups were used as controls. No significant differences were seen in the weekly weight gain between the litters of pregnant-lactating and lactating rats. In addition mammary glands of both groups were histologically identical. Yet, these groups showed a completely different mammary gland morphology, when they were compared with pregnants only. This study showed that, a new pregnancy carries a trivial influence on a well established lactation

Resonant frequency of middle ear

A new mathematical model has been developed to represent the middle ear. The natural frequency of the system comprising of the tympanic membrane and the ossicular chain is obtained. Comparison of the theoretical result with the experimental result shows excellent agreement