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Journal of Forensic Medicine ; (6): 196-203, 2006.
Artigo em Chinês | WPRIM | ID: wpr-983179

RESUMO

OBJECTIVE@#The STR genotypping of trace oral epithelial cells which are microdissected by laser capture microdissection system (LCM) is explored.@*METHODS@#The oral epithelial cells are microdissected using a low-power infrared laser by VERITAS Microdissection Instrument. STR loci of Profiler Plus are detected by multiplex PCR procesures.@*RESULTS@#DNA genotyping of 7-8 oral epithelial cells are succeeded, and DNA genotyping of 3-4 oral epithelial cells are failed.@*CONCLUSION@#It is viable in genotyping of trace oral epithelial cells by Laser Capture Microdissection as a new technology of seperating single cell.


Assuntos
Humanos , Separação Celular/métodos , DNA/genética , Células Epiteliais , Genótipo , Lasers , Microdissecção/métodos , Boca/citologia , Sequências de Repetição em Tandem
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