Clinical study of osteotome sinus floor elevation without grafting and with simultaneous implants placement / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the clinical results of osteotome sinus floor elevation (OSFE) without grafting combined with simultaneous implant placement.</p><p><b>METHODS</b>A total of 65 patients underwent maxillary sinus floor elevation from alveolus without any bone grafting from January 2000 to December 2008 and 96 implants were placed in the maxillary posterior edentulous region simultaneously. Clinical and radiography examinations were performed. The residual bone height ranged from 5 to 8 mm and the mean bone height was (6.78 ± 1.04) mm. The mean following period was 33.4 months. Statistical analysis was performed by chi square test.</p><p><b>RESULTS</b>Ninety-five of 96 implants were clinically stable and functioned without any pain and other complaints. One implant was extracted 15 days after operation because of mobility and the other implants obtained osseointegration. The mean implant protrusion length was 2.6 mm, ranging from 1 to 5 mm. Different degree of new bone formation was observed in 51 (54%) of implants. New maxillary sinus floor outline was observed in 33 (35%) of implants and there was no obvious new bone in 11 (12%) of implants. There was no significant deference between the implant protrusion length and sinus floor remodeling.</p><p><b>CONCLUSIONS</b>Under strict indications, the clinical results of OSFE without bone grafting combined with simultaneous implant placement were predictable in short term. The new sinus floor formation was not related to the implant protrusion length.</p>

Analysis on the imaging features of AIDS with pulmonary fungal infection / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Pulmonary fungal infection is one type of the common opportunistic infections in AIDS patients. The disease is hard to diagnose because of its complicated imaging features. The objective of this study was to investigate the imaging performance characteristics of pulmonary fungal infection in AIDS patients.</p><p><b>METHODS</b>Fifty-one patients with AIDS complicated with pulmonary fungal infection and 56 patients of non-AIDS with pulmonary fungal infection were examined by CT scans and high-resolution CT scans. The contrast enhanced scans were performed in patients with the mass or suspected enlarged mediastinal lymph nodes. Results were compared between the two groups.</p><p><b>RESULTS</b>The most common fungal infection in the two groups of patients was Candida albicans. The infection rates were 54.8% (28 cases) in the group (AIDS patients with pulmonary fungal infection) and 58.3% (32 cases) in another group (non-AIDS patients with pulmonary fungal infection). In the two groups, the difference in diffuse distribution and the difference in incidence of affected upper and lower lobes in the bilateral lung fields were statistically significant. The differences in patchy or large consolidation shadow, cavitas, enlarged lymph nodes in mediastinum and pleural effusion were also significant when comparing the two groups.</p><p><b>CONCLUSIONS</b>The lesion in most of AIDS patients with pulmonary fungal infection tends to exhibit diffuse distribution, patchy or large consolidation shadow covering a more extensive region. The differences between AIDS with pulmonary fungal infection and non-AIDS with pulmonary fungal infection are statistically significant in lesion location and complicated imaging features. The most common fungal infection in AIDS patients is Candida albicans.</p>

Effect of selenium and zinc on the proliferation of human esophageal cancer Eca109 cell line in vitro / 南方医科大学学报

<p><b>OBJECTIVE</b>To observe the effect of selenium and zinc alone or in combination on the growth and proliferation of human esophageal cancer Eca109 cell line in vitro.</p><p><b>METHODS</b>Different doses of sodium selenite and zinc sulfate were added into the culture medium of Eca109 cells and normal liver epithelial HL7702 cells (control), and the changes in the cell growth were assessed by means of cell growth curve, (3)H-thymidine incorporation assay and flow cytometry.</p><p><b>RESULTS</b>High-concentration selenium (0.3 micro g/ml) and zinc (3.5 microg/ml) alone both obviously inhibited Eca109 cell proliferation, and the inhibitory effect was enhanced by a combined treatment. At high concentrations, both selenium and zinc promoted HL7702 cell proliferation, but when combined, they produced inhibitory effect on the cell growth. Selenium and zinc at the physiological concentrations ( 0.1 microg/ml and 1.0 microg/ml, respectively) produced similar effects on Eca109 cells and the control cells. Selenium at 0.3 microg/ml caused Eca109 cell growth arrest in S phase, but this effect was not statistically significant; 3.5 microg/ml zinc significantly increased the number of Eca109 cells in G(1) phase. When combined, 0.3 microg/ml selenium and 3.5 microg/ml zinc caused significant G(1) arrest and promoted apoptosis of the cancer cells, an effect stronger than that of either of the agents used alone.</p><p><b>CONCLUSION</b>High-concentration selenium and zinc show a synergetic effect in inducing growth inhibition of human esophageal cancer Eca109 cell line possibly by causing cell cycle arrest and promoting cell apoptosis, and their combined use can be toxic to normal human liver epithelial cells.</p>

Effects of a regional Chinese diet and its vitamin supplementation on proliferation of human esophageal cancer cell lines / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>To study the effects of a local diet popular in Yanting region (YT diet) on the proliferation of two human cell lines (Eca-109 esophageal squamous cell carcinoma line and HL7702 normal liver epithelial cell line) in rats by a sero-physiological approach.</p><p><b>METHODS</b>Male SD rats were divided into six groups and fed respectively with a conventional diet and the YT diet (one of the five experimental diets) supplemented with two vitamin mixtures (Mix. 1: vitamins A, E, and folic acid; Mix.2: mix.1 plus riboflavin and vitamin C) at two different doses. On the 30th day, sera were collected from the rats and added into a medium for cell culture, with 10% FBS used as a serum control. The effects were assessed by MTT assay, DNA synthesis and flow cytometry assays.</p><p><b>RESULTS</b>Compared with the control, the sera from rats fed with the YT diet significantly promoted the proliferation of Eca-109 cells, which was, however, reversed by the supplementation with two vitamin mixtures at high doses. Surprisingly, the same treatment produced contrary effects on HL7702 cells as compared with Eca-109 cells.</p><p><b>CONCLUSION</b>The sera from rats fed with the YT diet could promote the proliferation of human esophageal cancer cell line Eca-109, whereas the sera from those fed with the YT diet supplemented with vitamin mixtures might have inhibitory effects on the proliferation of Eca-109 cells.</p>

Effect of two different desensitizers on bond strength of dentin bonding agents / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To evaluate the bond strength of total-etch or self-etch dentin bonding agents after using two different dentin desensitizers on exposed dentin and investigate the bond interface by scanning electron microscope (SEM).</p><p><b>METHODS</b>Thirty intact and non-carious human third molars were used. The occlusal enamel was removed with the use of a slow-speed saw under water cooling. These teeth were divided into three groups using a table of random numbers with 10 teeth each. These three groups were treated with water (Group C), UltraEZ (Group U) and MI Paste (Group M) respectively. Then 10 teeth from each group were divided into A subgroup (n = 5) bonded with Single Bond 2 adhesive system and B subgroup (n = 5) bonded with Xeno III adhesive system according to manufacturers' instructions. A block of composite resin was build up to 4-5 mm. All the teeth were sectioned occluso-gingivally to obtain bar-shaped specimens with bonded surface area about 0.9 mm x 0.9 mm. The tension of the sample was tested by a microtensile tester at 1 mm/min. The mean values of bond strength were compared using one-way ANOVA. Three samples were chosen randomly from each of six groups for SEM investigation.</p><p><b>RESULTS</b>There were no significant differences between Group U and Group C both in A and B subgroups. While there were significant differences between Group M and Group C in two bonding-agent subgroups. For SEM, the hybrid layer was thin and dense in six groups. Both total-etch and self-etch bonding systems could get fair resin tag infiltration in Group C and Group U. In Group M, the resin tags were relatively shorter and fewer than the anterior mentioned two groups.</p><p><b>CONCLUSIONS</b>UltraEZ had no effect on bond strength of both kinds of dentin bonding agents, while MI paste could diminish bond strength.</p>