Mediation of mitochondrial translocator protein by tanshinone ⅡA in apoptosis of HepG2 cells / 中国药理学通报

Aim To investigate the role of mitochondrial translocator protein(TSPO)in the apoptosis of HepG2 cells induced by tanshinone IIA(Tan II A)and the involved mechanism. Methods Following the HepG2 cells treated with Tan ⅡA at 2.5, 5 and 10 μmol·L-1, the cell viability was determined by MTT assay, and intracellular ATP content was determined by luciferin-luciferase method. Oxygen utilization was measured polarographically with a Clark oxygen electrode. Cell apoptosis was determined by Hoechst 33342 staining and flow cytometry. The mitochondrial membrane potential was assessed with JC-1 staining. The intracellular distribution of TSPO was examined by TSPO immunostaining, and the expressions of TSPO, Cyto C, caspase-3, caspase-9 were determined by immunoblotting analysis. Results Tan II A inhibited the proliferation of HepG2 cells in a dose-and time-dependent manner. The treatment with Tan II A inhibited ATP production and oxygen utilization of mitochondria. In addition, Tan ⅡA enhanced TSPO expression and accumulation in nuclei and up-regulated the expression of Cyto C, caspase-3 and caspase-9. Conclusions Tan II A induces the apoptosis of HepG2 cells, which may be related to the TSPO-mediated mitochondrial dysfunction.

Primary Medication Reconciliation Service Provided by Clinical Pharmacist for Chronic Kidney Disease in Our Hospital / 中国药学杂志

OBJECTIVE: To evaluate the efficacy of medication reconciliation (MR) service provided by clinical pharmacist for chronic kidney disease(CKD).METHODS: Patients with CKD administered in department of nephrology between Dec, 2017 and Mar. Total of 2018 who met the inclusion criteria were given MR service, and the newest Pharmaceutical Care Network Europe (PCNE) V8.02 classification scheme was constructed for drug related problems (DRP) to analysis and summary.RESULTS: MR was conducted 48 times (accounted for 80%) on 60 patients during the study period. Total of 59 medication DRP were identified. The most common DRP was treatment effectiveness (accounted for 84.75%), there were 54 causes (accounted for 76.06%) were prescribing in 71 causes, all the causes were classified as likely to cause level 1 harm (accounted for 84.75%), clinical pharmacists made 145 interventions, 102 (70.34%) of these were completely accepted and implement.CONCLUSION: DRPs are common in CKD patients, clinical pharmacists should provide MR for CKD patients, and conducted as routine work.

Analysis on Utilization of TCM Injections for Activating Blood Circulation to Dissipate Blood Stasis in Lanzhou Second People's Hospital During 2014-2016 / 中国中医药信息杂志

Objective To provide references for clinical rational utilization of TCM injections for activating blood circulation to dissipate blood stasis. Methods Retrospective analysis was used to sort and analyze in terms of species, consumption sum, DDDs and DDDc of TCM injections for activating blood circulation to dissipate blood stasis in Lanzhou Second People's Hospital (here in after referred to as our hospital) during 2014‐2016. Results The consumption sum of TCM injections for activating blood circulation to dissipate blood stasis was relatively high in the consumption sum of TCM injections (over 30％), and the type of TCM injections for activating blood circulation to dissipate blood stasis kept stable (up to 9‐11) in our hospital during 2014‐2016. The annual growth rate of consumption sum in 2016 (-5.42％) was significantly lower than it in 2015 (11.63％). Danshen Injection featured high selectivity and economical in the clinical application (DDDs: 12 600 in 2015, 6600 in 2016; DDDc: 32.70 in 2015, 28.46 in 2016; sorting ratio: 5.00 in 2015, 3.00 in 2016). Conclusion The clinical application of TCM injections for activating blood circulation to dissipate blood stasis tends to be rational in our hospital. Danshen Injection features high selectivity and economical in the clinical application. Danhong Injection features high selectivity but the poor economical.

Clinical observation on treatment of mycotic vaginitis with Sophora gel combined with Fluconazole capsules / 中国中药杂志

Mycotic vaginitis is a common and frequently-occurring gynaecopathia and easy to attack repeatedly, so painful to patients. In this study, the authors observed the clinical efficacy of Sophora gel combined with Fluconazole capsules in treating mycotic vaginitis, in order to seek an effective method for treating mycotic vaginitis. Totally 85 patients with mycotic vaginitis treated in our hospital between December 2012 and July 2014 were randomly divided into the treatment group (43 patients) and the control group (42 patients). The treatment group was given vaginally Sophora gel (one piece every night for 14 days) and orally Fluconazole capsules (150 mg, once every three days, four times in total); The control group was only administered with Fluconazole capsules. The total efficacy, cure rate, recurrence rate and clinical symptom improvements of the two groups were observed. The results show that the total efficacy, the cure rate and the recurrence rate of the treatment group vs. the control group were respectively 97.7%, 90.7% and 2.6% vs. 83.3%, 71.4% and 20.0%, with statistical significance in their differences (P < 0.05). The treatment group showed reduced leucorrhea, pruritus vulvae disappearance and earlier mucosal hyperemia disappearance than the control group, with statistical significance in their differences (P < 0.05). In conclusion Sophora gel combined with Fluconazole capsules can improve antifungal activity of drugs, relieve clinical symptoms, shorten the course of disease, enhance the cure rate and reduce the recurrence rate; So this therapy can be widely applied in clinic.

Epidemiological analysis of maternal death in Beijing from 1995 to 2010 / 中华预防医学杂志

<p><b>OBJECTIVE</b>To analysis the trend of maternal death time and explore the impact of the variety of death causes and birth place to maternal death time.</p><p><b>METHODS</b>According to the data provided by Beijing Maternal and Children Health Hospital, the 372 death cases of pregnant and lying-in women from 1995 to 2010, a retrospective study was performed to analyze the death causes, maternal death time and the influencing factors.</p><p><b>RESULTS</b>The MMR declined from 27.9 per 100 000 live births from 1995 to 2000 to 14.8 per 100 000 live births from 2006 to 2010, with a decline of 46.9%. Among the maternal death within 24 hours of delivery, 79.7% (106/133) died of obstetric hemorrhage, hypertensive disorder complicating pregnancy and amniotic fluid embolism. It took up 47.8% (64/134) from 1995 to 2000, reduced to 37.5% (45/120) from 2006 to 2010. At the same time, the maternal mortality ratio within 24 hours reduced from 40.2%(54/134) to 28.3% (34/120), the variation of death time was consistent with the causes of maternal mortality (χ² = 59.109, P < 0.05). Indirect obstetric causes increased significantly from 2006 to 2010, 53.2% (33/62) of pregnant women with heart disease, cerebrovascular disease and pulmonary embolism died in prenatal or more than 120 hours postnatal. Among the maternal death delved in hospital, 29.0% (29/100) died within 24 hours, 52 cases delved at home or in private clinics, 43 cases (82.6%) died within 24 h postnatal. There were significant differences between birth place and death time (χ² = 24.500, P < 0.05).</p><p><b>CONCLUSION</b>Maternal death time changed from 24 hours of delivery to prenatal or postnatal a long time. The maternal mortality causes and hospital delivery is an important factor affecting maternal time.</p>

Study on maternal deaths in Beijing, from 1996 to 2010 / 中华流行病学杂志

Objective To provide evidence for further reducing the maternal mortality rate (MMR) through analyzing the causes of death and influencing factors on the issue.Methods Every maternal death from 1996 to 2010 was audited by experts and relevant information was collected and analyzed,retrospectively.Results (1) The overall MMR among Beijing residents was 20.2 per 100 000 live births in 1996-2000 while decreased to 14.2 per 100 000 live births from 2006 to 2010.At the same time,the MMR of migrating people decreased from 47.7 to 15.2 per 100 000 live births.(2) The proportion of women having received middle school education and above,increased from 59.8％ to 78.8％ and the non-prenatal care maternal ratio decreased from 39.1％ to 12.7％.(3) Among the 349 deaths in the period of 1996-2010,209 (59.9％) were caused by direct obstetric reasons.Proportion of obstetric hemorrhage declined from 14.4％ to 9.2％ and the amniotic fluid embolism declined from 20.7％ to 15.0％.Prolific,non-prenatal care and private clinics/home deliveries were important factors on direct obstetric reasons.71.4％ maternal mortality of indirect causes appeared abnormal during pregnancy.(4) The WHO twelve-grade classification standard on maternal deaths was adopted.Our data showed that the main reasons causing maternal deaths of Beijing residents were related to the skills of medical staffs (62.4％) and healthcare management (19.7％).The main reasons of maternal deaths among migrating people would include:poor knowledge (41.4％),inappropriate attitude(32.3％) and resources of the families(24.0％).Conclusion The MMR in Beijing continuously declined from 1996 to 2010.However,in order to keep up with the changing causes related to maternal deaths as well as to the increasing service requirements,it is necessary to develop a new model on service and management of the issue.