RESUMO
<p><b>OBJECTIVE</b>To explore the utility of Principal Factor Analysis (PFA) in chromatographic data for quality control.</p><p><b>METHOD</b>Chromatographic fingerprints of processed root pieces of Paeonia lactiflora were determined by HPLC, the PFA was used for data processing.</p><p><b>RESULT</b>The quantitative differences among different growing areas and different processing batches were found with the method.</p><p><b>CONCLUSION</b>The method could be used in quality control for monitoring between-batch products of traditional Chinese pharmaceutical process.</p>
Assuntos
Cromatografia Líquida de Alta Pressão , Métodos , Ecossistema , Análise Fatorial , Temperatura Alta , Pinellia , Química , Preparações de Plantas , Química , Padrões de Referência , Raízes de Plantas , Química , Plantas Medicinais , Química , Controle de Qualidade , Reprodutibilidade dos Testes , Tecnologia Farmacêutica , Métodos , VinhoRESUMO
<p><b>OBJECTIVE</b>To develop an HPLC method for the determination of hyodeoxycholic acid in Bile Arisaema.</p><p><b>METHOD</b>The methanol extracts were separated on a Diamonsil ODS column eluted at 35 degrees C with a mixture of methanol-water-glacial acetic acid (75:25:0.01) at flow-rate 1.0 mL x min(-1). The drift-tube temperature of ELSD was 85 degrees C, and the flow rate of air was 3.0 L x min(-1).</p><p><b>RESULT</b>Hyodeoxycholic acid in methanol extract was well separated, the relationship of logarithms of injection amount and peak area was linear (r = 0.9998) over the range of 2.4-24 microg. The average recovery was 97.1% (RSD 2.9%, n=5).</p><p><b>CONCLUSION</b>The method could be used for quality control of Bile Arisaema.</p>
Assuntos
Animais , Arisaema , Química , Bile , Química , Cromatografia Líquida de Alta Pressão , Métodos , Ácido Desoxicólico , Combinação de Medicamentos , Temperatura Alta , Luz , Medicina Tradicional Chinesa , Padrões de Referência , Tubérculos , Química , Plantas Medicinais , Química , Controle de Qualidade , Reprodutibilidade dos Testes , Espalhamento de RadiaçãoRESUMO
<p><b>OBJECTIVE</b>To provide experimental data for the quality control of processed Paeonia lactiflora, a Chinese herbal medicine.</p><p><b>METHOD</b>Traditional processing of P. lactiflora was simulated, content of paeoniflorin and water extracts among different preparations were assayed by HPLC; The quantitative correlations among different processing conditions were analyzed, the effects of processing parameters on the contents of paeoniflorin and water extracts were assayed a nanalysed.</p><p><b>RESULT AND CONCLUSION</b>The controlled processing parameters were correlated with covariables which showed that processing procedures was controllable, and the heating temperature was a factor impacting the content of paeoniflorin.</p>
Assuntos
Benzoatos , Hidrocarbonetos Aromáticos com Pontes , Glucosídeos , Temperatura Alta , Monoterpenos , Paeonia , Química , Plantas Medicinais , Química , Controle de Qualidade , Tecnologia Farmacêutica , Métodos , TemperaturaRESUMO
<p><b>OBJECTIVE</b>To provide the experimental data for the quality control of Paeonia lactiflora a prepared Chinese medicinal herb.</p><p><b>METHOD</b>The contents paeoniflorin among different growing areas and different processing methods methods were assayed by HPLC.</p><p><b>RESULT</b>The quantitative differences of paeoniflorin content among different growing areas and different processing methods wereanalyzed quantitatively.</p><p><b>CONCLUSION</b>The quantitative differences among different growing areas were not significant and the quantitative differences among different processing methods within 10%.</p>