RESUMO
To search for potential drugs with potent antitussive, expectorant, antiasthmatic activities and low toxicity, a series of verticinone-bile acids salts were prepared based on the clearly elucidated antitussive, expectorant and antiasthmatic activities of verticinone in bulbs of Fritillaria and different bile acids in Snake Bile. The antitussive, expectorant and antiasthmatic activities of these verticinone-bile acid salts were then screened with different animal models. Ver-CA (verticinone-cholic acid salt) and Ver-CDCA (verticinone-chenodeoxycholic acid salt) showed much more potent activities than other compounds. The bioactivities of Ver-CA and Ver-CDCA are worthy to be intensively studied, and it is also deserved to pay much attention to their much more potent antitussive effects than codeine phosphate. In order to elucidate whether they have synergistic effect and attenuated toxicity, their activities will be continuously compared with single verticinone, cholic acid and chenodeoxycholic acid at the same doses on different animal models. The application of "combination principles" in traditional Chinese medicinal formulations may be a novel way in triditional Chinese medicine research and discovery.
Assuntos
Animais , Feminino , Masculino , Camundongos , Antiasmáticos , Química , Farmacologia , Antitussígenos , Química , Farmacologia , Asma , Ácidos e Sais Biliares , Química , Farmacologia , Cevanas , Química , Farmacologia , Ácido Quenodesoxicólico , Química , Farmacologia , Ácido Cólico , Química , Farmacologia , Tosse , Combinação de Medicamentos , Composição de Medicamentos , Métodos , Sinergismo Farmacológico , Expectorantes , Química , Farmacologia , Fritillaria , Química , Cobaias , Plantas Medicinais , Química , Distribuição Aleatória , SerpentesRESUMO
<p><b>OBJECTIVE</b>To study the constituents from roots of Euphorbia hylonoma.</p><p><b>METHOD</b>Column chromatographic techniques were used for isolation and purification of the chemical constituents and their structures were identified by spectral analysis (IR, 1H-NMR, 13C-NMR, 2D-NMR and MS).</p><p><b>RESULT</b>Six compounds were isolated and elucidated as nonane (1), bis (2-ethylhexyl) phthalate (2), euphol (3), beta-sitosterol (4), acalyphol (5) and daucosterol (6) respectively.</p><p><b>CONCLUSION</b>Compounds 1, 2, 3, 5 and 6 were isolated from the plant for the first time.</p>
Assuntos
Alcanos , Química , Dietilexilftalato , Química , Euphorbia , Química , Lanosterol , Química , Raízes de Plantas , Química , Plantas Medicinais , Química , Sitosteroides , Química , Triterpenos , QuímicaRESUMO
<p><b>AIM</b>To establish a fingerprint analysis method of Fritillaria hupehensis.</p><p><b>METHODS</b>fingerprint was performed by HPLC-ELSD. Hypersil ODS column was used; the mobile phase was composed of methanol (with 0.05% triethylamine) and water with gradient elution; flow rate was 1.0 mL x min(-1); recording time was 60 min; drift tube temperature was 75 degrees C; gas flow rate was 1.9 L x min(-1).</p><p><b>RESULTS</b>HPLC fingerprint of Fritillaria hupehensis was obtained.</p><p><b>CONCLUSION</b>A reliable method was provided for controlling the quality of Fritillaria hupehensis.</p>