RESUMO
OBJECTIVE@#To analyse the var gene repertoire and characterise the chondroitin sulphate A (CSA)-binding activity of the Duffy-binding like (DBL) domains encoded by the var2csa gene of a Plasmodium falciparum (P. falciparum) isolate in Hainan Province, China.@*METHODS@#The sequences of var DBL1 regions were PCR-amplified, sequenced and the sequence characteristics was bioinformatically analysed. Recombinant proteins encoded by the var2csa genes were expressed and purified. The binding activities of the recombinant proteins to CSA receptor was detected by ELISA assays.@*RESULTS@#Fifty six unique DBL α sequences were obtained, and the sequences represented similar diversity to the var genes of the genome parasite 3D7. There are two var2csa genes in the P. falciparum isolated from Hainan Province. Unlike in other falciparum parasites such as HB3, the two var2csa genes are more diverged. The receptor-binding capacity of DBL-5ε and DBL-6ε domains of HN var2CSA was studied.@*CONCLUSIONS@#This work represented the diversity of var genes of a P. falciparum isolate in China.
Assuntos
Animais , Humanos , Antígenos de Protozoários , Genética , Metabolismo , China , Sulfatos de Condroitina , Genética , Metabolismo , Clonagem Molecular , DNA de Protozoário , Ensaio de Imunoadsorção Enzimática , Malária Falciparum , Genética , Metabolismo , Dados de Sequência Molecular , Plasmodium falciparum , Química , Genética , Domínios e Motivos de Interação entre Proteínas , Proteínas de Protozoários , Genética , Metabolismo , Receptores de Superfície Celular , Genética , MetabolismoRESUMO
An outbreak of fever and dyspnea with high incidence rate and case fatality rate occurred among pigs in a pigfarm in Jilin province, China, in 2000. The paramyxovirus-like particles could be observed in lungs, spleens and kidneys of the dead pigs under the transmission electron microscope. A Newcastle disease virus (NDV) isolate designated as JL01 was determined as the causal agent for the disease outbreak. The purified virus was reinoculated into pigs, and then the pigs infected with the virus showed similar symptoms, and the HI antibody of NDV could be detected from the reinoculated pigs. The MDT, ICPI and EID50 of the JL01 isolate was 55.2h, 1.60 and 10(-7.5)/0.1 mL respectively. The F gene of JL01 was cloned and sequenced, and the results showed that the identities of F gene shared by JL01 and avirulent NDV reference strains were from 91.5% to 98.5%, and could be ascribed to NDV genotype I. Thus, the swine NDV JL01 strain should be an avirulent strain with gene variation, but the virulence of JL01 was just the same as velogenic strains.
Assuntos
Animais , Cães , Camundongos , Coelhos , Galinhas , Cavalos , Microscopia Eletrônica , Doença de Newcastle , Virologia , Vírus da Doença de Newcastle , Classificação , Genética , Filogenia , Reação em Cadeia da Polimerase , Análise de Sequência de DNA , Suínos , Doenças dos Suínos , Virologia , Proteínas Virais , GenéticaRESUMO
A coxsackievirus B strain was successfully isolated by cells culture from cardiac muscle tissues of a dead Sichuan golden monkey with myocarditis from a zoo of Changchun in China. The isolate was consistent with CVB by morphology, physicochemistry test, animal regression test and RT-PCR. Analysis of VP1 partial gene sequence and detection of mice specific serum IgG showed that the strain isolated was a coxsackievirus B3. It was the first CVB case report in Sichuan golden monkey and the strain isolated was named CVB/SGM-05.
Assuntos
Animais , Camundongos , Chlorocebus aethiops , Enterovirus Humano B , Haplorrinos , Virologia , Coração , Virologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Células Vero , Proteínas Estruturais Virais , GenéticaRESUMO
The expression of the hemagglutinins of Avian influenza virus H5 H7and H9 subtypes was studied in this article by Pichia pastoris, one of the eukaryotis expression systems. Three reconstructed expression plasmids and engineering strains, named pPIC9K-H5HA, pPIC9K-H7HA, pPIC9K-H9HA and GS115/pPIC9K-H5HA, GS115/pPIC9K-H7HA, GS115/pPIC9K-H9HA repectively, were obtained. The reconstructed yeast engineering strains were identified by MD and MM plate selecting and PCR. The induced interests proteins were examined by SDS-PAGE and Western-bloting,the results showed that the interest genes were expressed exactly. And this will be helpful in the future study of antigen detection and antibody detection kit, as well in the subunit vaccines developing.