Network analysis of microRNAs, transcription factors, and target genes involved in axon regeneration / 浙江大学学报（英文版）（B辑：生物医学和生物技术）

Axon regeneration is crucial for recovery from neurological diseases. Numerous studies have identified several genes, microRNAs (miRNAs), and transcription factors (TFs) that influence axon regeneration. However, the regulatory networks involved have not been fully elucidated. In the present study, we analyzed a regulatory network of 51 miRNAs, 27 TFs, and 59 target genes, which is involved in axon regeneration. We identified 359 pairs of feed-forward loops (FFLs), seven important genes (Nap1l1, Arhgef12, Sema6d, Akt3, Trim2, Rab11fip2, and Rps6ka3), six important miRNAs (hsa-miR-204-5p, hsa-miR-124-3p, hsa-miR-26a-5p, hsa-miR-16-5p, hsa-miR-17-5p, and hsa-miR-15b-5p), and eight important TFs (Smada2, Fli1, Wt1, Sp6, Sp3, Smad4, Smad5, and Creb1), which appear to play an important role in axon regeneration. Functional enrichment analysis revealed that axon-associated genes are involved mainly in the regulation of cellular component organization, axonogenesis, and cell morphogenesis during neuronal differentiation. However, these findings need to be validated by further studies.

Analysis of 5-year-follow-up of pediatric blood oncology patients with intrathecal injection of methotrexate mixed microcontent vincristine / 中华实用儿科临床杂志

Objective To analyze the clinical manifestation,auxiliary examination,treatment,prognosis of 16 cases who experienced inadvertent injection of intrathecal microcontent vincristine,and and they were followed up for 5 years.Methods To collect detailed medical record material for 5 years of 16 pediatric blood oncology patients who appeared with nervous system damage symptoms caused by inadvertent administration of microcontent vincristine intrathecally,combined with subsequent visits at the clinic,or through telephone and letters followed-up.Results Clinical manifestations of lumbosacral radicular dysfunction were found in all 16 cases,among them 2 cases also had cervicothoracic radicular damage symptoms.The first sign was weakness in lower limbs,subsequently,walking difficulty.Cerebrospinal fluid protein was higher than normal in 3 cases.Magnetic resonance imagine of the brain and spinal cord revealed demyelination in the white matter of 2 cases.Electromyographic revealed neurogenic damage and showed complete or partial denervation changes in 11 cases that received this examination.After the treatment consisted of medication use to improve nerve nutrition metabolism and promote nervous function recovery,combined with positive rehabilitation therapy,13 cases had improvement from level Ⅰ to Ⅲ in myodynamia.Of 9 cases with neurogenic bladder,4 cases were back to normal,3 cases were improved to different extent.Till Oct.2012,6 cases lived in remission,9 cases including 6 cases of recurrence died of the primary disease or complication in the treatment process,and 1 case died of penicillin anaphylactic shock almost 1 year after chemotherapy.Conclusions Inadvertent intrathecal injection of microcontent vincristine can cause lumbosacral radicular dysfunction.Trace amounts of vincristine intrathecally cumulative can also cause serious damage to the nervous system.The early application of horrmone and nerve nutrition drugs combined with positive rehabilitation therapy have surely curative effect to alleviate the neuropathy for inadvertent administration of microcontent vincristine intrathecally.

Investigations on cordycepin production by solid culture of Cordyceps militaris / 中国中药杂志

An efficient method to produce cordycepin by solid culture using Cordyceps militaris was investigated in this study. Firstly, the changes of cordycepin during various growing periods of solid culture using 5 strains of C. militaris were detected, the best strain and optimal growing period for cordycepin production were determined. Then, by experiments of quadratic rotation-orthogonal combination design and orthogonal design, the medium composition and growth conditions for high yield of cordycepin were optimized. With the optimized method to produce cordycepin, the content of cordycepin in the medium was increased to 0.60%, which was nearly 2 times higher than the highest yield reported.

Change rate in bone cells of mice / 中国针灸

<p><b>OBJECTIVE</b>To study the anti-mutation action of acupuncture and moxibustion.</p><p><b>METHODS</b>Mice were randomly divided into 6 groups, group 1 (normal control group), group 2 (positive control group), group 3 (prevention group I), group 4 (prevention group II ), group 5 (treatment group I) and group 6 (treatment group II). The mice in the group 2-6 were treated by cyclophosphamide (ip, 50 mg/kg body weight), and in the 3'-6 groups were given acupuncture at "Zusanli" (ST 36) and moxibustion at "Guanyuan" (CV 4). At the end of experiment, all the mice were decapitated and chromosome aberration rate and sister chromatid exchange rate of bone marrow cells were investigated.</p><p><b>RESULTS</b>The chromosome aberration rate and the sister chromatid exchange rate of bone marrow cells in the positive control group increased significantly as compared with the normal control group, while they decreased significantly in the group 3, 4, 5, 6 as compared with the positive control group (P < 0.01).</p><p><b>CONCLUSION</b>Acupuncture and moxibustion have anti-mutation action, inhibiting the increase of chromosome aberrations and sister chromatid exchange of bone marrow cells in mice induced by cyclophosphamide.</p>