Influence of three different anesthesia protocols on aged rat brain: a resting-state functional magnetic resonance imaging study / 中华医学杂志(英文版)

BACKGROUND@#Resting-state functional magnetic resonance imaging (rs-fMRI) is a promising method for the study of brain function. Typically, rs-fMRI is performed on anesthetized animals. Although different functional connectivity (FC) in various anesthetics on whole brain have been studied, few studies have focused on different FC in the aged brain. Here, we measured FC under three commonly used anesthesia methods and analyzed data to determine if the FC in whole brain analysis were similar among groups.@*METHODS@#Twenty-four male aged Wistar rats were randomly divided into three groups (n = 8 in each group). Anesthesia was performed under either isoflurane (ISO), combined ISO + dexmedetomidine (DEX) or α-chloralose (AC) according to the groups. Data of rs-fMRI was analyzed by FC in a voxel-wise way. Differences in the FC maps between the groups were analyzed by one-way analysis of variance and post hoc two-sample t tests.@*RESULTS@#Compared with ISO + DEX anesthesia, ISO anesthesia caused increased FC in posterior brain and decreased FC in the middle brain of the aged rat. AC anesthesia caused global suppression as no increase in FC was observed.@*CONCLUSION@#ISO could be used as a substitute for ISO + DEX in rat default mode network studies if the left temporal association cortex is not considered important.

Carriage of drug resistance genes and homology of carbapenem-resistant Acinetobacter baumannii isolated from respiratory intensive care unit / 中国感染控制杂志

Objective To investigate drug resistance genes and epidemic characteristics of β-lactamase carried by carbapenem-resistant Acinetobacter baumannii (CRAB) in the respiratory intensive care unit(RICU) in a hospital.Methods Clinically isolated CRAB from RICU patients in October-December 2015 were collected.Five drug resistance genes (KPC-2,IMP,VIM,NDM-1,OXA-23) were specifically amplified by polymerase chain reaction (PCR),amplified products were performed agarose gel electrophoresis and sequencing analysis,the homology was analyzed with pulsed-field gel electrophoresis (PFGE).Results A total of 22 CRAB strains were isolated in October-December 2015,19 (86.36％) of which were isolated from sputum.The resistance rate of 22 CRAB strains to compound sulfamethoxazole was 59.09 ％,resistance rate to minocycline was 9.09 ％,all were sensitive to polymyxin B,resistance rates to other antimicrobial agents were more than 80％.Three kinds of resistance genes KPC-2,IMP and NDM-1 were not found by PCR amplification,positive rates of VIM and OXA-23 were both 100％.PFGE homology analysis revealed that 22 strains were divided into 13 different types,each type contained 1-5 strains,9 types(69.23％) contained only 1 strain respectively,the other 4 types (30.77％) contained 2-5 strains.A5,A7,and A8;A9,A11,A14,A19 and A22;A4,A10 and A12;A16 and A18 were of the same type respectively.Conclusion The main types of β-lactamase-resistant genes of CRAB in RICU are VIM and OXA-23.Homology analysis shows a small parts are of the same clone strains,which reveals epidemic of a small scale.

Influence of resveratrol on the growth of Lewis lung carcinomas in mice and its underlying mechanism / 肿瘤

Objective: To explore the influence of resveratrol (Res) on the growth of Lewis lung carcinomas in mice and the corresponding mechanism. Methods: The Lewis lung carcinoma model was established in C57BL mice. Forty C57BL mice were randomly divided into 4 groups (n = 10): control group, low-dose Res group (2.5 mg · kg-1 · d-1), middle-dose Res group (5 mg · kg-1 · d-1) and high-dose Res group (10 mg · kg-1 · d-1). Mice were given intragastrically with Res for 20 d. All mice were sacrificed on d 22 after inoculation. The volume and weight of tumors were recorded. Microvessel density (MVD) in tumor tissues was measured by immunohistochemistry. The expression level of vascular endothelial growth factor (VEGF) was examined by immunohistochemistry and RT-PCR. The apoptotic index (AI) was determined by TUNEL assay. Results: The tumor growth was suppressed significantly in Res 5 mg and 10 mg · kg-1 · d-1 groups. The weights and volumes of Lewis lung carcinoma in both groups above were markedly decreased compared with control group (P 0.05). Conclusion: Res remarkably inhibites the growth of Lewis lung carcinoma in mice by inhibiting expression of VEGF, reducing MVD, and promoting apoptosis.

Inhibitory effect of quercetin on cervix cancer in mice / 中南大学学报(医学版)

OBJECTIVE@#To investigate the antitumor effect and mechanism of quercetin on murine cervical carcinoma U14.@*METHODS@#The 615-strain mice with U14 cervical cancer cells were randomly divided into 4 groups: a control, a low-dose intervention group [1.5 g/(kg . d)], a middle-dose intervention group [3.0 g/(kg . d)], and a high-dose intervention group [6.0 g/(kg . d)]. Different treatments were inoculated intraperitoneally after 6 days of transplantation and all mice were sacrificed after 26 days. The weight of tumors and inhibitory rates were measured. The expression levels of microvessel density (MVD) and nuclear factor-kappaB (NF-kappaB) were detected by immunohistochemistry. The apoptosis index (AI) was measured by terminal deoxynucleotidyl transferase assay in situ (TUNEL).@*RESULTS@#Compared with the control group, the tumor growth in the high-dose intervention group was suppressed significantly, and the weight and volume of the tumor were markedly decreased (P0.05).@*CONCLUSION@#Quercetin showed a marked inhibitive effect on U14 growth, and its antitumor mechanism may be associated with inhibiting the angiogenesis and inducing apoptosis.