Simultaneous determination of 4 major components in semen cassiae obtusifoline by HPLC / 中国中药杂志

<p><b>OBJECTIVE</b>To establish a HPLC method for the simultaneous determination of 4 major components in semen cassiae obtusifoline and provide valuable data for quality control of semen cassiae obtusifoline.</p><p><b>METHOD</b>Kromasil 100-5 C18 column (4.6 mm x 250 mm, 5 microm). Eluted with water: acetonitrile: tetramethylene oxide: glacial acetic acid (100: 23: 5:1). Detecte at 278 nm. The flow rate was 1.0 mL x min(-1).</p><p><b>RESULT</b>Four compositions were separated well. Good linearies were obtained within the range of 0.274-1.37 microg, 0.153-0.765 microg, 0.302-1.51 microg and 0.052-0.26 microg for rubrofusarin gentiobioside, casside, aurantio-obtusin-6-O-beta-D- glucopyranoside, casside B respectively. The average recoveries were 100.9% (RSD 1.8%),101.2% (RSD 1.2%), 99.40% (RSD 2.2%), 100.5% (RSD 1.6%).</p><p><b>CONCLUSION</b>The method is accurate, reliable and convenient. It can be used to control the quality of semen cassiae obtusifoline and its products.</p>

Personal Exposure to PM2.5 of Children Living near Traffic Road and the Influencing Factors in Beijing / 环境与健康杂志

Objective To evaluate personal exposure level to PM2.5 of children living in one of districts of Beijing and to explore the possible influencing factors. Methods Thirty-six pupils were chosen from a primary school in the district. The PM2.5 level of classrooms,campuses and children's living rooms were monitored by Aerosol Monitor and 24 h time-active pattern and environmental information of living room of these pupils were collected at the same time. Each child's exposure level of PM2.5 was calculated by combining the environment PM2.5 monitor data and the children's 24 h time-active pattern. The possible influencing factors were analyzed by single factor analysis(Kruskal-Wall rank sum test and Spearman rank correlation analysis) and multiple linear regression models. Results The 24 h average exposure level of PM2.5 of 36 pupils was 98.13 ?g/m(341.22～121.13 ?g/m3) . The single factor analysis showed that the personal exposure level to PM2.5 was significantly related with air pollution index(API) (r =0.836,P