Identification of Long Non-Coding RNAs and Their Target Genes from Mycelium and Primordium in Model Mushroom Schizophyllum commune

Schizophyllum commune has emerged as the most promising model mushroom to study developmental stages (mycelium, primordium), which are two primary processes of fruit body development. Long non-coding RNA (lncRNA) has been proved to participate in fruit development and sex differentiation in fungi. However, potential lncRNAs have not been identified in S. commune from mycelium to primordium developmental stages. In this study, lncRNA-seq was performed in S. commune and 61.56 Gb clean data were generated from mycelium and primordium developmental stages. Furthermore, 191 lncRNAs had been obtained and a total of 49 lncRNAs were classified as differently expressed lncRNAs.Additionally, 26 up-regulated differently expressed lncRNAs and 23 down-regulated between mycelium and primordia libraries were detected. Further, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that differentially expressed lncRNAs target genes from the MAPK pathway, phosphatidylinositol signal, ubiquitin-mediated proteolysis, autophagy, and cell cycle. This study provides a new resource for further research on the relationship between lncRNA and two developmental stages (mycelium, primordium) in S. commune

Influences of initiation of epidural analgesia pain relief in early labor on uterine myoelectrical activity / 中华围产医学杂志

Objective To assess the influences of early implementation of patient-controlled epidural analgesia (PCEA) in labor on uterine myoelectrical activity and delivery outcomes. Methods A prospective study was conducted on 240 singleton cephalic primiparae with spontaneous labor at Guangzhou Women and Children's Medical Center from January 2015 to October 2018. Those women, who were ready to accept PCEA, were randomly assigned to early- or late-PCEA group based on cervical dilation of 0-3 cm or 3-6 cm at the time of commencing PCEA, while those who refused PCEA in labor were classified as non-PCEA group. Uterine electromyographic activity and visual analogue score (VAS)were recorded before and 1 h and 2 h after PCEA. Patient satisfaction with labor, duration of the first stage of labor, volume of postpartum bleeding within 2 h after delivery and neonatal Apgar score were compared between different groups using multivariate analysis of variance, repeated measures analysis of variance, LSD-t test or Chi-square test. Results The VAS values 1 h after PCEA in the early- and late-PCEA group were both lower than that in the non-PCEA group (2.08±1.34 and 2.00±1.28 vs 7.65±1.04, LSD-t were － 27.713 and － 27.663, P<0.001) and those before PCEA (7.65±0.91 and 7.62±0.86, LSD-t were －32.879 and －33.349, P<0.001). The VAS values 2 h after PCEA in the early- and late-PCEA group were both lower than that in the non-PCEA group (1.63±1.53 and 1.41±1.56 vs 7.66±0.87, LSD-t were －27.018 and －27.823, P<0.001) and those before PCEA (LSD-t were －31.379 and －32.718, P<0.001).The patient satisfaction rate with labor was higher in the early-PCEA group comparing to the late-PCEA group [80.0% (72/90) vs 61.1% (55/90), P<0.001], and the two figures above were both higher than that of the non-PCEA group [20.0% (12/60), both P<0.001]. There was no significant difference in the duration of the first stage of labor, the volume of postpartum blood loss 2 h after delivery, oxytocin usage rate, the rate of convertion to cesarean section, neonatal birth weight or Apgar score at 1 or 5 min among the three groups (all P>0.05). There was also no significant difference in uterine electromyographic parameters among the three groups before or 2 h after PCEA (all P>0.05). The number and duration of burst, power density spectrum peak frequency, root mean square and total power 1 h after PCEA in the early- and later-PCEA group were all lower than those in the non-PCEA group [4.80±2.49 and 5.54±3.04 vs 9.67±2.44; (34.41±1.21) and (36.94±1.18) vs (41.68±1.53) s; (0.36±0.08) and (0.36±0.07) vs (0.48±0.05) Hz ; (0.05±0.04) and (0.05±0.05) vs (0.07±0.05) mV; (4.33±0.51) and (5.36 ±0.59) vs (9.90±1.43) pV2; all P<0.05]. Conclusions The effect of PCEA on uterine myoelectrical activity has no association with the commencing time. While early PCEA could alleviate the labor pain as soon as possible, which enable us to improve the efficacy of labor analgesia, patient satisfaction and maternal and neonatal safety without increasing cesarean section rate.

Discussion on Chinese Herbal Compound Patent Drafting / 中国中医药信息杂志

As China is the birthplace of traditional Chinese medicine, herbal compound is an important part of Chinese traditional culture, and is the field with China’s most obvious advantage and national features to participate in international competition. This article provided several suggestions according to the compound patent application document drafting including specification,claim of rights, and“novelty, creativity, practicability”investigation angle in order to enhance quality of herbal compound patent writing.

Effect of reinforced health education on deep radiofrequency thermotherapy for patients with tumor / 中南大学学报(医学版)

OBJECTIVE@#To explore the effect of reinforced health education on deep radiofrequency thermotherapy for patients with tumor.@*METHODS@#From June 2012 to June 2014, 106 patients who underwent deep radiofrequency thermotherapy in our hospital were randomly selected, and were divided into a control group (n=69) and an observation group (n=65). The observation group received reinforced health education while the control group received the traditional health education before treatment. Th e reinforced health education included preparation, cooperation and health behavior during and aft er treatment. Th en the compliance rate, degree of satisfaction, and the awareness rate of related knowledge were compared and analyzed aft er treatment between the 2 groups.@*RESULTS@#Th e compliance rate in the observation group and the control group was 85.51% and 63.08% respectively; there was significant difference in the awareness rates of related knowledge and the overall degree of satisfaction between the patients and their family members (all P<0.05).@*CONCLUSION@#Implement of reinforced health education is benefit to patients to understand the content of health education before treatment and keep health behavior after treatment, and can also improve the compliance, the degree of satisfaction in the deep radiofrequency thermotherapy for patients with tumor.

Monitoring of Drug-resistance in Klebsiella pneumoniae in a Oil Field Hospital / 中华医院感染学杂志

OBJECTIVE To investigate drug-resistance status of Klebsiella pneumoniae(KPN) among oil field staff-workers and provide the evidence for clinical application of antibiotics.METHODS Referring to the National Clinical Laboratory Operation Rules,KPN strains were isolated and identified.The drug-sensitivity testing was performed by K-B methods.The extended-spectrum-?-lactamases(ESBLs) producers were detected by double-disc synergy test and disc confirming test.RESULTS The 276 strains of KPN were mainly isolated from respiratory tract and urinary tract.The isolating rate of ESBLs-producing KPN was 37.0%.The results of susceptibility testing showed the drug-resistance to common antibiotics in ESBLs producers was generally higher than that in ESBLs nonproducer.There was no KPN resistant to imipenem and meropenem.CONCLUSIONS Drug-resistance status of KPN in a oil field hospital is very serious.We should strengthen monitoring and controlling of it.

Distribution and Drug Resistance of Pseudomonas aeruginosa in ICU / 中华医院感染学杂志

OBJECTIVE To approach the distribution and drug resistance of Pseudomonas aeruginosa(PAE) in nosocomial infection in intensive care unit(ICU) and provide the evidence for clinical controlling of it.METHODS Clinical data of nosocomial infection with PAE isolated from ICU from Jan 2005 to Dec 2008 were investigated retrospectively.The results of susceptibility test in 277 PAE strains were analyzed statistically.RESULTS PAE strains were mostly isolated from respiratory tract(56.0%).The drug resistance rates to ceftriaxone,cefotaxime and gentamicin were all above 64.0%.PAE was resistant to the other antibiotics in different degrees.The suceptibility rates to imipenem,meropenem,amikacin,cefoperazone/sulbactam,piperacillin/tazobactam and levofloxacin were all above 74.0%.CONCLUSIONS The clinician should use the antibiotics reasonably according to etiological testing to elevate the level of anti-infection treatment.

Measurement of endothelium-dependent dilation function of brachial arteries in patients with diabetic nephropathy and its implication / 中华内分泌代谢杂志

Type 2 diabetic patients were divided to 3 groups according to their urinary albumin excretion rate: simple diabetic group (n=30), early diabetic nephropathy group (n=24) and clinical diabetic nephropathy (n=20). Endothelium-dependent dilation function of brachial artery was measured by high resolving power colour Doppler echography and circulating endothelial cells in blood were counted. The results showed that endothelium-dependent dilation function of brachial artery in 3 groups declined progressively in the order of simple diabetic group, early diabetic nephropathy group and clinical diabetic nephropathy group, suggesting that this measurement may serve as one parameter reflecting the status of vascular endothelial function and the extent of vascular lesion.