The therapeutic effect of G-CSF-mobilized autologous stem cells on radiation pulmonary injury in mice / 中华放射医学与防护杂志

Objective To investigate the effects of G-CSF-mobilized autologous stem cells in the prevention of radiation pulmonary injury.Methods Mice were divided into control group,irradiation group and treatment group.Mouse model of pulmonary fibrosis was established by exposing chest to a single dose of 14 Gy.Animals in the treatment group received recombinant human G-CSF (250 μg/kg daily for 5 d) before the irradiation in order to mobilize autologous stem cells in vivo.The general condition and mortality were documented after radiation injury.The pathological study with histological scoring,Masson staining and Sirius red staining with polarized light analysis were used to identify lung injury and the potential benefit of stem cell mobilization.Results Local chest irradiation of a single dose of 14 Gy was a suitable dose to create radiation-induced pulmonary fibrosis in mice.The death rate was 37.5％,which mainly happened around 11 weeks after injury.In contrast,all of the animals in G-CSF treated group survived.The ratio of lung to body mass was significantly increased in both irradiation group and treatment group (F =23.20,P＜0.05) around 3 months after the injury,with a higher ratio in irradiation group than that in treatment group (P＜0.05).Histological scoring for alveolar inflammation at 3 months after injury revealed statistically significant difference in irradiation group and treatment group compared with control group (F=11.93,P＜ 0.05).At this time point,the pathological observation showed lung tissue degeneration and necrosis with alveolitis and interstitial inflammation,as well as fibroblasts proliferation and focal collagen deposition in alveolar septa.At 4 month after the injury,the inflammation ininterstitial tissue was receded,but fibrosis and collagen deposition were significantly increased.In addition,at 3 and 4 months afterinjury,the pulmonary fibrosis was aggravated in irradiation group (F=28.73,16.85,P＜0.05),and significantly alleviated in the treatment group (P＜0.05).The similar results were confirmed in collagen content analysis (IOD) by Sirius red staining and image analysis (F =17.70,17.79,P＜ 0.05).Conclusions Autologous mobilization of stem cells could prevent the death of radiation-injured animals possibly by alleviating early lung injury and interstitial inflammation as well as the late pulmonary fibrosis,suggesting a therapeutic potential of autologous stem cell mobilization in radiation pulmonary fibrosis.

Trend of caesarean section rate and puerpera characteristics: based on Robson classification / 中华流行病学杂志

Objective To analyze the trend in caesarean section rate and puerpera characteristics in hospital,and provide valuable information for maternal and child health policy making and clinical practice.Methods A total of 12 041 women who delivered in the affiliated Chaohu Hospital of Anhui Medical University from October 1,2010 to September 30,2016 were selected.Based on Robson classification system,changes in the rate of caesarean delivery as well as its relationship with two-child policy and infant sex ratio were analyzed.Results The overall caesarean section rate gradually decreased from 66.9％ to 44.2％ during the past six years.Respectively,the caesarean section rate in primiparae with singleton term babies decreased to 32.1％ and the rate in multiparas without uterine scar decreased to 14.2％,and the rate in premature delivery decreased to 22.9％,the differences were significant (P＜0.01).The proportion of vaginal delivery (R1,R3),multiparas with uterine scar (R5) and twins pregnancy (R8) increased,the differences were significant (P＜0.01).The annual overall newly-born sex ratio ranged from 110:100 to 128:100.In group R1,more babies were girls,the proportion was stable,more women with premature delivery and multiparas had boy babies,but the boy babies by multiparas without uterine scar obviously decreased in the last 2 years.Conclusions Primiparae with singleton head birth,multipara without uterine scar and women with premature deliveries are the key population in the effort of reduction of caesarean section rate.The caesarean section rate and proportion were unstable in multiparas with uterine scar,breech deliveries and twin deliveries.The application of Robson classification system can improve the comparability of the surveillance data.

Trend of caesarean section rate and puerpera characteristics: based on Robson classification / 中华流行病学杂志

Objective To analyze the trend in caesarean section rate and puerpera characteristics in hospital,and provide valuable information for maternal and child health policy making and clinical practice.Methods A total of 12 041 women who delivered in the affiliated Chaohu Hospital of Anhui Medical University from October 1,2010 to September 30,2016 were selected.Based on Robson classification system,changes in the rate of caesarean delivery as well as its relationship with two-child policy and infant sex ratio were analyzed.Results The overall caesarean section rate gradually decreased from 66.9％ to 44.2％ during the past six years.Respectively,the caesarean section rate in primiparae with singleton term babies decreased to 32.1％ and the rate in multiparas without uterine scar decreased to 14.2％,and the rate in premature delivery decreased to 22.9％,the differences were significant (P＜0.01).The proportion of vaginal delivery (R1,R3),multiparas with uterine scar (R5) and twins pregnancy (R8) increased,the differences were significant (P＜0.01).The annual overall newly-born sex ratio ranged from 110:100 to 128:100.In group R1,more babies were girls,the proportion was stable,more women with premature delivery and multiparas had boy babies,but the boy babies by multiparas without uterine scar obviously decreased in the last 2 years.Conclusions Primiparae with singleton head birth,multipara without uterine scar and women with premature deliveries are the key population in the effort of reduction of caesarean section rate.The caesarean section rate and proportion were unstable in multiparas with uterine scar,breech deliveries and twin deliveries.The application of Robson classification system can improve the comparability of the surveillance data.

Construction of eukaryotic expression vector of mouse microRNA miR-21 and identification its expression activity in 293 cells / 局解手术学杂志

Objective To construct the eukaryotic expression vector for mouse microRNA miR-21 and identification its expression activ-ity in 293 cells. Methods The genomic sequence containing pre-miR-21 was amplified from mouse genomic DNA by PCR and cloned into the pRC/CMV plasmid. The constructed recombinant plasmid pRC/CMV-mmu-miR-21 was transfected to 293 cells by lipofectamine 2000, and the stably transfected cells were screened with G418,from which total RNA was extracted for detecting the expression of mature miR-21 by northern blot. In the meantime,a luciferase report plasmid examing the activity of miR-21 named pmiR-21-Luc reporter was also construc-ted,and luciferase activity analysis indicated the product of pRC/CMV-mmu-miR-21 indeed had biological activity. Results Both restriction enzyme digestion analysis and sequencing proved the recombinant plasmids were constructed correctly. The miR-21 was highly expressed in the screened clones of 293 cells and it had good biological activity. Conclusion The eukaryotic expression plasmid of mouse miR-21 was successfully constructed,which laid the foundation of further investigation of the role of miR-21 during skin wound healing.

The phenomenology research of perioperative therapeutic communication for rectal cancer patients / 中国实用护理杂志

Objective To explore the clinical practice and patient experience of perioperative therapeutic communication in rectal cancer patients, in order to provide theoretical basis for the further clinical application of therapeutic communication. Methods To adopt phenomenology method of qualitative research, collecting data by the direct participation, observation, half structural formula thorough interview and other methods. To establish coding and refine theme. Results Four themes of the rectal cancer patients experience of perioperative therapeutic communication were generalized: emotional adaptation, actively coping with surgery, intraoperative cooperation, postoperative rehabilitation and self-care. Conclusions Application of therapeutic communication system can effectively interfere with patients cognitive behavior, improve communication ability of nurses and extended the nursing service connotation.

Effect of nordihydroguaiaretic acid on expressions of VEGF and its receptor KDR in vitro / 第三军医大学学报

Objective　To explore the effect of nordihydroguaiaretic acid (NDGA) on the expressions of vascular endothelial growth factor (VEGF) and its receptor, kinase-inserted domain containing receptor(KDR) and the possible mechanism. Methods　The expression of VEGF in human malignant glioma cell line SHG-44 and that of KDR in human umbilical vein endothelial cell (HUVEC) line ECV-304 were observed 1～3 d after NDGA treatment with immunohistochemistry, in situ hybridization and image analysis. Results　The expression of VEGF was declined at protein or mRNA levels in SHG-44 cells after treated with 100 μmol/L NDGA for 1 to 3 d. The expression of KDR in endothelial cells with 100 μmol/L NDGA treatment for 1 to 3 d was decreased too, in a more obvious way compared with the decline of VEGF expression in SHG-44 cells. Conclusion　The results suggest that NDGA inhibits the expression of VEGF in glioma cells as well as that of VEGF receptor KDR in endothelial cells, which may be the important molecular mechanism of anti-angiogenesis of NDGA.

Effect of nordihydroguaiaretic acid on the morphological changes of endothelial cells in vitro / 第三军医大学学报

Objective　To investigate morphological changes of endothelial cells after nordihydroguaiaretic acid (NDGA) treatment in vitro. Methods　The morphological changes of human umbilical vein endothelial cells (HUVEC) cell line ECV-304 and the cell apoptosis rate in sub-G0 phase were observed with invert, light and electron microscope and flow cytometry after NDGA treatment at different concentrations or with PBS (0.01 mol/L) as control. Results　①After the treatment of NDGA at 50～200 μmol/L for 1～3 d or up to 8 d at 100 μmol/L, ECV-304 cells tended to elongate into a shuttle-like sparse appearance and those in mitosis were decreased, indicating the suppression of cell proliferation. All these alteration was in a time-and dose-dependent manner. ②NDGA-treated ECV-304 cells displayed morphological features of apoptosis, especially at the 48th h after the treatment. With flow cytometry, the cells in sub-G0 phase were significantly increased, and reached its peak at hour 12 (20.42%) after NDGA treatment. In addition, the degeneration and necrosis of ECV-304 cells were related to the concentrations of NDGA. Conclusion　NDGA can inhibit the proliferation and growth of endothhelial cells, and induce apopotosis, which might also inhibit angiogenesis.

Design of new type thermal container for infant / 医疗卫生装备

Objective To develop infant thermal container with forced protection circuit so as to ensure its security. Methods Temperature database was set in thermal container and Cygnal F005 was used as intelligent controller. The state of the sensor was judged by comparison precisely. Double temperature controller was adopted to ensure normal working temperature. When the container went wrong, it could automatically switch to protection circuit to keep the temperature for infant. Results With precise temperature control and steady working station, the new type thermal container for infant could provide a safe and comfortable condition with clean air and fitting temperature and humidity. Conclusion The container is steady, accurate, safe and convenient.