RESUMO
Herpesvirus infections in Cervidae are a serious threat affecting some deer species worldwide. In our attempt to identify malignant catarrhal fever-associated herpesviruses in deer herds, ten gammaherpesviral DNA fragments were identified in five species of deer in herds in China by using a pan-herpesvirus polymerase chain reaction assay targeting viral DNA polymerase. Notably, in sambar (Rusa unicolor), a novel gamma-2 herpesvirus was identified that showed a close relationship with fallow deer lymphotropic herpesvirus (LHV), while the other fragments were phylogenetically grouped together with Elk-LHV. Determination of whether these viruses have any clinical implication in these deer species should be undertaken urgently.
Assuntos
Animais , Bovinos , China , Cervos , DNA , DNA Viral , Infecções por Herpesviridae , Herpesviridae , Febre Catarral Maligna , Reação em Cadeia da PolimeraseRESUMO
Objective To optimize the preparation technology of ?-cyclodextrin inclusion complex of Oleum Viticis Negundo.Methods Orthogonal test was applied.With the inclusion rate and the content of 1,8-Cineole as the parame ters,L9(34) table was used to examine the effects of 4 factors:the proportion of Oleum Viticis Negundo and ?-cyclodextrin,inclusion temperature,inclusion time and the proportion of Oleum Viticis Negundo and water.Results The best condition was∶oil ∶ ?-cyclodextrin∶ water=1∶8∶70(ml∶g∶ml),stirring for 1.5 hour at 50 ℃.Conclusion Under the optimized condition,inclusion rate of Oleum Viticis Negundo is over 80%.