Relationship between inhibition of ongoing responses with attention deficit and behavior disorder / 中国学校卫生

Objective@#To examine the relationship between inhibition of ongoing responses deficit with attention deficit and behavior disorder among ADHD children.@*Methods@#Totally 43 children with attention deficit hyperac tivity disorder (ADHD) aged 6-14 years who met the DSM-V criteria for ADHD were recruited. And 38 health children were recruited as the control group. Vision Continuous Performance Task (CPT) and Matching Familiar Figures Test were used to assess attention deficit and behavior disorder feature of the ADHD children. Stop-signal paradigm was used to assess the inhibition of ongoing responses.@*Results@#In the stop-signal paradigm, the ADHD children’s error rate was significantly higher than typically developing children(t=4.34, P<0.01). The ADHD children error rate in the stopsignal task was positively correlated with the wrong number miss rate in CPT task(r=0.45, P<0.01), similar finding was observed in regression analysis,with inhibition of ongoing responses accounted for 20.1% of attention deficit(t=3.21,P<0.01). In ROC curve analysis, the cut-off sore was 15.50 in the task. The error rate in stop-signal task showed no association with miss rate in Matching Familiar Figures Test(r=0.09,P>0.05).@*Conclusion@#Inhibition of ongoing responses among ADHD children is associated with attention deficit, but not with existence of behavior disorder.

Ophthalmology rotation for general practice residency training / 中华全科医师杂志

Ophthalmology rotation is an integral part of standardized training for general practitioners. The duration of ophthalmology training is only half a month in whole 33-month general practice residency training. The main objective of training for general practice residents is to master the basic clinical skills of ophthalmology, to be able to accurately and timely identify eye diseases, especially those requiring urgent treatment, and to prevent the loss of visual function. This article introduces our teaching experience in ophthalmology training for general practice residents accumulated since 2014. We suggest that the trainees should pre-examine patients independently, play a role of standard patients and receive consultation from teachers. We developed a simple patient assessment form for primary care doctors, the patient who got 10 or more points of the form, should be referred to ophthalmological specialists.

Ophthalmology rotation for general practice residency training / 中华全科医师杂志

Ophthalmology rotation is an integral part of standardized training for general practitioners. The duration of ophthalmology training is only half a month in whole 33-month general practice residency training. The main objective of training for general practice residents is to master the basic clinical skills of ophthalmology, to be able to accurately and timely identify eye diseases, especially those requiring urgent treatment, and to prevent the loss of visual function. This article introduces our teaching experience in ophthalmology training for general practice residents accumulated since 2014. We suggest that the trainees should pre-examine patients independently, play a role of standard patients and receive consultation from teachers. We developed a simple patient assessment form for primary care doctors, the patient who got 10 or more points of the form, should be referred to ophthalmological specialists.

In vitro differentiation of spermatogonial stem cells using testicular cells from Guangxi Bama mini-pig

In this study, we attempted to establish a culture system for in vitro spermatogenesis from spermatogonial stem cells (SSCs) of Bama mini-pig. Dissociated testicular cells from 1-month-old pigs were co-cultured to mimic in vivo spermatogenesis. The testicular cells were seeded in minimum essential medium alpha (α-MEM) supplemented with Knockout serum replacement (KSR). Three-dimensional colonies formed after 10 days of culture. The colonies showed positive staining for SSC-associated markers such as UCHL1, PLZF, THY1, OCT4, Dolichos biflorus agglutinin, and alkaline phosphatase. Induction of SSCs was performed in α-MEM + KSR supplemented with retinoic acid, bone morphogenetic protein 4, activin A, follicle-stimulating hormone, or testosterone. The results showed that STRA8, DMC1, PRM1, and TNP1 were upregulated significantly in the colonies after induction compared to that in testis from 1-month-old pigs, while expression levels of those genes were significantly low compared to those in 2-month-old testis. However, upregulation of ACROSIN was not significant. Replacement of α-MEM and KSR with Iscove's modified Dulbecco's medium and fetal bovine serum did not upregulate expression of these genes significantly. These results indicate that SSCs of Bama mini-pig could undergo differentiation and develop to a post-meiotic stage in α-MEM supplemented with KSR and induction factors.

The effect analysis of clinical pathway management for capillary bronchitis / 中国基层医药

Objective To analyze the effect of clinical pathway management for capillary bronchitis in Department of Pediatrics.Methods 75 children with capillary bronchitis after the implementation of clinical pathway management were selected as the observation group,and 68 children with capillary bronchitis before the implementa-tion of clinical pathway management were selected as the control group.The hospitalization cost,average hospital stay, antibiotic use rate,cure rate,nonsocomial infection rate,rehospitalization rate within two weeks,service satisfaction and other comprehensive indices were compared.Result The total cost of drug of the observation group[(1 198.49 ± 397.48)yuan]was lower than (1 324.05 ±376.57)yuan of the control group(Z =2.00,P 0.05).Conclusion Clinical pathway management for capillary bronchitis in Department of Pediatrics of primary hospital can play a role in standardizing medical practice,reducing average hospitalization days,controlling medical cost,and improving service satisfaction under the precondition of ensuring medical quality.