Analysis of Composition Principles of Prescriptions for Simple Obesity Based on Traditional Chinese Medicine Inheritance Support System / 广州中医药大学学报

Objective To explore the composition principles of prescriptions for simple obesity based on the analysis of medical literature issued in recently 30 years about Chinese medicine for simple obesity. Methods We collected literature of Chinese medicine in treating simple obesity from VIP, CNKI, Wanfang, CBM and CMCC/CMCI databases, and screened out the formulas for simple obesity to establish a formula database. And then the medication rules were figured out with the Traditional Chinese medicine Inheritance Support System version 2.5 (TCMISS 2.5) software. Results A total of 57 formulas were screened out. And the top 10 herbs with highest occurrence frequency in the prescriptions were Poria, Rhizoma Atractylodis Macrocephalae, Rhizoma Alismatis, Radix et Rhizoma Rhei, Fructus Crataegi, Radix Astragali, Radix Glycyrrhizae, Folium Nelumbinis, Pericarpium Citri Reticulatae and Semen Cassiae. After data mining, we got 13 high-frequency herb-pair combinations and 31 core combinations, and 8 new prescriptions were extracted. Conclusion The research has achieved the quantitative description of the relationship between the Chinese medicines, the extraction of the core combinations and the discovery of new Chinese medicine prescriptions for simple obesity. The results will provide references for the development of new Chinese medicines for the treatment of simple obesity .

Effects of glucose variation on hexose monophosphate shunt and leucocyte respiratory burst in patients with type 2 diabetes / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the effect of blood glucose instability on respiratory burst of leukocytes in patients with type 2 diabetes (T2DM).</p><p><b>METHODS</b>Forty-five patients with T2DM were divided into 3 groups after continuous glucose monitoring for 72 h with glucose wavy coefficient <1.5 (n=11), between 1.5 and 3.0 (n=19), and >3.0 (n=15). Peripheral blood neutrophils were isolated from the diabetic patients and normal control subjects for assay of glucose 6-phosphate dehydrogenase (G6PD) with a spectrophotometric method, detecting G6PD mRNA expression by real-time PCR, and determining reactive oxygen species level using the fluorescent probe DCFH-DA.</p><p><b>RESULTS</b>Compared with the normal control group, the diabetic patients showed significantly lowered G6PD activity (F=78.739, P<0.05) and ROS level (F=384.962, P<0.05) but significantly increased G6PD mRNA expression (F=269.612, P<0.01). These changes were significantly correlated with the blood glucose wavy coefficients.</p><p><b>CONCLUSION</b>The fluctuation of blood glucose in T2DM patients can decrease G6PD activity and lead to functional decline of the respiratory burst.</p>

Effects of glucose concentration on G6PD activity and respiratory burst of normal human′s neutrophils in vitro / 重庆医学

Objective To determine the effects of glucose concentration on G 6PD activity and respiratory burst of normal hu-man′s neutrophils in vitro .Methods Normal human′s neutrophils were cultured in different glucose concentration for 8 hours ,as-sayed G6PD activity by spectrophotometric method and determining ROS content by fluorescent probe DCFH-DA .Results G6PD activity and ROS of 15 mmol/L group and 25 mmol/L group were significant lower than before ,when the 5 mmol/L group and L-GLU group didn′t have significant change with time goes by .And G6PD activity and ROS of 25 mmol/L group were the lowest in all groups(P<0 .01) .Conclusion High glucose may induce G6PD activity decreased and cause respiratory burst dysfunction as a stimulating factor .The stimulation intensity was increased with the increase of glucose concentration .It′s the probable mechanism on susceptibility to infections in patients with diabetes mediated by dysfunction of respiratory burst in leucocyte .

Protein kinase A signaling pathway participates in high glucose-induced inhibition of G6PD activity and respiratory burst dysfunction in THP-1 cells / 南方医科大学学报

<p><b>OBJECTIVE</b>To observe the changes in glucose 6-phosphate dehydrogenase (G6PD) activity, cAMP and respiratory burst function in THP-1 cells exposed to high glucose and identify the possible signaling pathways to mediate these changes.</p><p><b>METHODS</b>THP-1 cells were treated with high glucose, high glucose plus the PKA inhibitor (PKI), or normal glucose plus Forskolin. The changes in the G6PD activity and cAMP in the exposed cells were assayed using the spectrophotometric method, and the reactive oxygen species (ROS) content in the cell culture was determined using the fluorescent probe DCFH-DA. Western blotting was employed to examine the expression of phosphorylated p47(phox) in the cells.</p><p><b>RESULTS</b>Compared with the normal control cells, the cells exposed to high glucose and to normal glucose and Forskolin showed a significantly lowered G6PD activity, ROS content and expression of phosphorylated p47(phox), but with a increased cAMP content (P<0.01). High glucose exposure in the presence of PKI caused no significant changes in G6PD activity, ROS level, phosphorylated p47(phox) or cAMP compared to those in the normal control cells (P>0.01).</p><p><b>CONCLUSION</b>High glucose causes inhibition of G6PD activity in THP-1 cells via activation of PKA and thus leads to respiratory burst dysfunction, which is the probable mechanism underlying the lowered leucocyte function and susceptibility to infections in diabetic patients.</p>

Synergistic Action of Fuming-Washing Therapy with Qingbixifang on Rheumatoid Arthritis with Heat Retention / 广州中医药大学学报

[Objective] To investigate the synergistic action of fuming-washing therapy with Qingbixifang ( a herbal lotion mainly composed of Herba Siegesbeckiae, Cortex Phellodendri, Radix Paeoniae Rubra, Radix Rehmanniae, Radix Stephaniae Tetrandrae, Rhizoma Atractylodis, Herba Ecliptae, Nidus Vespae, Olibanum, Myrrha, etc.) on rheumatoid arthritis (RA) with heat retention. [Methods] Sixty patients of RA with heat retention were randomized into groups A and B. Group A ( n = 30) was treated by fuming and washing the affected part with Qingbixifang, and taking Tongbiling Tablets and nimesulide; group B (n = 30) was treated by taking Tongbiling Tablets and nimesulide only. The treatment course of the two groups lasted one month. The time of morning stiffness, grip strength, the number of joints with tenderness, tenderness index, resting pain in joints, the number of swelling joints, swelling index, evaluation by patients themselves and by the doctor were compared before and after treatment. Laboratory parameters such as blood rheumatoid factor (RF), C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), white blood cell (WBC) count and platelet (PLT) count were also observed before and after treatment. [Results] The total effective rate was 90.0% in group A and 66.7% in group B, the difference being significant (P