Application value of standardized training of mobile nurses in operating room nursing / 中国实用护理杂志

Objective:To explore the application value of establishing standardized mobile nurses in operation room in improving the quality of operation nursing and reducing the occurrence of adverse events.Methods:A total of 3 445 operations in the Eastern and Western surgical wards of Shengjing Hospital Affiliated to China Medical University from 2018 to 2019 were selected in this study. The surgeries performed in the eastern surgical wards were the experimental group ( n = 1 650), and the standardized mobile nurse operation mode was adopted. The surgeries performed in the Western surgical wards were the control group ( n=1 795) adopted the general operation management and cooperation mode. The differences of nursing quality score, incidence of adverse events and satisfaction of doctors and nurses under different operation modes were observed. Results:The scores of surgical preparation, instrument management, surgical safety, and environmental management in the quality of surgical care in the experimental group were 9.14±0.38, 9.42±0.37, 9.64±0.27, 9.64±0.27, respectively, which were higher than those of the control group 9.11±0.36, 7.99±0.33, 8.03±0.39, 7.98±0.79, the difference between the two groups was statistically significant ( t value was 2.379-139.712, P<0.05). The satisfaction rate of doctors in the test group was 97.33% (73/75), which was higher than 91.67% (52/60) in the control group, and the satisfaction rate of nurses in the test group was 99.00% (99/100), which was higher than 97.92% (90/96) in the control group. The difference between the two groups was statistically significant ( χ2 value was 5.530, 3.920, P<0.05). The incidence of adverse surgical events in the experimental group was 0.79%(13/1 650), which was less than 1.78%(32/1 795) in the control group, the difference was statistically significant ( χ2 value was 6.600, P<0.05). Conclusions:The establishment of mobile nurse database in the operating room can not only alleviate the current situation of human resource shortage in the operating room, improve the quality of surgical care and physician satisfaction, but also reduce the probability of adverse events.

Application of Case-Based Learning teaching mode based on WeChat public platform in nursing teaching practice in operation room / 中国实用护理杂志

Objective To explore the application effect of Case-Based Learning(CBL) teaching mode based on WeChat public platform in nursing practice teaching in operating room. Methods From July 2016 to June 2018, 132 nursing students in the operating room of Shengjing Hospital of China Medical University were selected as experimental subjects. They were randomly divided into the traditional teaching group (control group, from July 2016 to June 2017) and WeChat public platform combined with CBL group (experimental group, from July 2017 to June 2018 ). On the basis of traditional teaching and based on the WeChat public platform, the experimental group were sent the content of the CBL case based on the syllabus of the courses to students one week before the internship. At the end of the teaching, a theoretical written examination and skill operative quiz were performed to assess the effects of teaching and satisfaction of the subjective learning experience. Results The scores of the theoretical and basic skills of the experimental group were (42.33 ± 8.26) and (45.17 ± 6.79) points, respectively. The control group was (37.28 ± 7.70) and (39.23 ± 5.43) points, respectively. The difference between the two groups was statistically significant (t=2.289 , 2.150, P<0.05). The overall satisfaction of the experimental group was 90.90％ (60/66), and the overall satisfaction of the control group was 57.58％ (38/66). The difference between the two groups was statistically significant (χ2=6.339, P <0.05). Conclusions The WeChat public platform assisted CBL teaching model in the operating room nursing practice improved the learning efficiency, helped to deepen understanding, and can be adopted gradually in large teaching hospitals for clinical practical teaching of operation room for nursing students.

MRI and MR cholangiopancreatography characteristics of cystic biliary atresia / 中国医学影像技术

Objective To analyze MRI and MR cholangiopancreatography (MRCP) manifestations of cystic biliary atresia (CBA). Methods MRI and MRCP data of 24 CBA infants confirmed with operation and pathology were retrospectively analyzed. MRCP and MRI findings were observed combining with intraoperative radiography and pathological features. Results Cystic structure in the hepatic hilum were found in all 24 infants. T2WI high signal in periportal area was showed in 11 infants. The width of the gallbladder was obviously shorter than the length in 23 infants, while gallbladder was absent in 1 infant. Fibrous plaque in the hepatic portal area was observed in 5 infants. Cystic structure located at hepatic hilum in 22 infants, at the left or right hepatic duct area in 2 infants. The cysts of the hepatic portals area were interlinked with the cystic duct in 18 infants. Conclusion Hepatic hilar cyst, hilar fiber block, morphology of gallbladder and common bile duct in MRCP are important signs for diagnosis of CBA. MRCP has a certain value in early diagnosis of CBA.

Imaging examination and diagnosis of airway foreign body in children / 实用医学杂志

Objective To evaluate the value of imaging examination and diagnosis of airway foreign body in children. Methods 218 of airway foreign bodies confirmed by bronchoscopy were retrospectively analyzed in terms of clinical and imaging data. Results 218 patients included 140 males and 78 females. Their ages ranged 6 months to 12 years(mean,19.2 months). All patients had conventional radiographs on frontal chest films,neck lateral films and fluoroscopy. 34 cases underwent 64-slice spiral chest CT examination. According to the location of the foreign bodies,29 cases were in throat and subglottic,55 cases in trachea and 134 cases in bronchus(79 cas-es in the right,55 cases in the left). According to the character of the foreign bodies,5 cases were metal,29 cas-es in bone,93 cases in peanuts,59 cases in seeds,22 cases in fruits and vegetables and 10 cases in others respec-tively. Thirty-fourcases showed a direct sign of the foreign body and 177 cases showed the indirect signs. 34 cases on CT examination directly were showed foreign body in the tracheal bronchi. The diagnostic accuracy of CT exami-nation was 100%. Conclusions X-ray photograph and fluoroscopy were the first choice for aspiratory foreign bod-ies in children. CT examination is an important supplementary method when conventional X-ray examination is neg-ative.

Surgical way of pregnancy termination for patients with placenta previa during midtrimester / 局解手术学杂志

Objective To investigate the effect of rivanol induction on pregnancy termination for patients with placenta previa during midtrimester.Methods From January 2010 to December 2015,16 patients of placenta previa underwent pregnancy termination induced by rivanol during midtrimester were regarded as the observation group, and 22 patients with normal placental position were regarded as the control group.The delivery time,amount of postpartum hemorrhage within 24 hours,one-time success rate of induced abortion,caesarean due to massive haemorrhage and postoperative infection of the two groups were recorded to analyze the clinical effect of rivanol.Results There was no statistically significant differences in the success rate,delivery time and caesarean due to massive haemorrhage between the two groups(P>0.05).The amount of intrapartum and postpartum hemorrhage in the observation group was more than that of the control group,with a statistically significant difference(P<0.05),but it was less than 500 mL,which did not significantly increase the related risk for patients.Conclusion Induced abortion by rivanol is a simple,safe and effective method for patients with placenta previa during midtrimester with fewer side effects and less trauma,which is the preferred method for such patients.

MSCT diagnosis of tumors of intra-abdominal cryptorchidism in children / 中国医学影像技术

Objective To explore the diagnostic value of MSCT in tumors of intra-abdominal cryptorchidism in children.Methods MSCT findings of 8 children with tumors of intra-abdominal cryptorchidism confirmed by surgery and pathology were analyzed retrospectively.Results Six tumors located in the right,2 (1 tumor of left cryptorchidism turned to the right abdominal) in the left.Eight children showed ovoid soft tissue tumor in abdomen.Three children displayed the long axis of the tumors consistent with regular descending course of embryonic testes.Six teratomas manifested as the cystic and solid mass with fat,calcification (ossification) insidey.Two yolk sac tumors manifested as the large cystic and solid mass with irregular necrosis and abundant tumor vessels.Conclusion The pathologic types of tumors for intra-abdominal cryptorchidism in children are different from adult.Most of them are teratomas or yolk sac tumors,and have some characteristics in MSCT.MSCT is helpful in diagnosis of tumor for intra-abdominal cryptorchidism with medical history.

CT Diagnosis of Chest Wall Rhabdomyosarcoma in Children / 中国医学影像学杂志

PurposePrimary chest wall rhabdomyosarcoma (RMS) is very rare with limited imaging characteristic studies in the literature. This paper analyzes the CT imaging features of chest wall RMS in children to improve the diagnostic accuracy.Materials and MethodsThe imaging data of contrast enhanced CT scan of pathology conifrmed chest wall RMS in ifve children were analyzed.ResultsThe lesion was located in the anterior chest wall in one case, in the posterior chest wall in two cases, and the lateral chest wall in two cases (axillary). The tumors were round or spindle in shape with shallow spiculation. Plain CT showed heterogeneous density with patchy low-density necrotic area in two cases, and homogeneous attenuation in three patients. In all ifve cases there was no calciifcation or fatty tissue. The tumor involvement of adjacent spinal canal was seen in one case. Visceral compression was evident including lung parenchyma in one case, heart and liver in one case. Tumor blood vessel growth was seen in two cases. All ifve lesions were adjacent to the ribs, humerus, scapula and the spine with bone destruction in one case. On contrast enhanced scan, all ifve cases demonstrated heterogeneous mild to moderate enhancement, more prominent in the periphery. There were enlarged feeding arteries. Necrotic areas did not enhance. In two cases there were pulmonary metastases. Pleural effusion and ascites were identiifed in one case. There was lymph node metastasis in one case.ConclusionThe CT manifestation of children's chest wall RMS for chest wall include large soft tissue mass, heterogeneous density, no calciifcation or fatty tissue, partial necrosis, adjacent tissue compression, lymph node or distant metastasis. Combining with clinical manifestations, comprehensive analysis of contrast enhanced CT imaging can improve diagnostic accuracy.

Expression of anti-gp96 scFv fragment in Pichia pastoris and identification of its biological activity / 生物工程学报

Secretory anti-gp96 scFv fragment was expressed in Pichia pastoris to obtain a small molecule antibody that specifically recognizes heat shock protein gp96. The gp96-scFv fragment gene was synthesized and cloned to Pichia pastoris expression plasmid pPICZa-A. Pichia pastoris X33 was electroporated with the linearized recombinant expression vector, and expression of gp96-scFv fragment was induced by methanol. The His-tagged recombinant protein was then purified by affinity chromatography and analyzed with SDS-PAGE and Western blotting assays. The biological activities of recombinant gp96-scFv fragment were determined by Western blotting, Immunofluorescence, ELISA and FACS assays. The gp96-scFv fragment was expressed successfully in Pichia pastoris. About 50 mg of recombinant protein could be purified from 1 liter of the Pichia pastoris culture supernatant. Its molecular weight was about 15 kDa. The gp96-scFv fragment could specifically bind to gp96 protein by Western blotting, immunofluorescence, ELISA and FACS analyses. Pichia pastoris-expressed gp96-scFv fragment specifically recognizes gp96 protein, which could be used for Western blotting, Immunofluorescence, ELISA and FACS analyses.

Comparison of pathological changes of lung tissue in rat pulmonary arterial hypertension model induced by two different doses of monocrotaline / 吉林大学学报(医学版)

Objective To establish rat models with pulmonary artery hypertension induced by monocrotaline(MCT) and to observe the pathological changes of lung tissue.Methods Seventy male Wistar rats were randomly devided into three group:control group(n=10),50 mg?kg-1 MCT group(n=30),60 mg?kg-1 MCT group(n=30).At two weeks and four weeks after injected intraperitoneally with MCT(injected intraperitoneally with equal normal saline in control group),the right ventricular systolic pressure(RVSP)and right ventricle weight/left ventricle+septum weight [RV/(LV+S)] ratio were measured.Hematoxylin-eosin staining and orcein technique were used to observe the pathological changes of lung tissue and pulmonary arterioles'medial thickness.Results Two weeks or four weeks after MCT administration,RVSP in 50 mg?kg-1 MCT group was higher than that in control group(respectively 36.6 mmHg?5.1 mmHg,39.1 mmHg?7.0 mmHg versus 26.1 mmHg?3.8 mmHg,both P

Culture and identification of smooth muscle cells induced with canine bone marrow-derived mesenchymal stem cells in vitro / 吉林大学学报(医学版)

Objective To explore the possibility of differentiation of canine bone marrow-derived mesenchymal stem cells(BMSCs) into smooth muscle cells(SMCs) and the potential of using these SMCs as cell sources for engineering of blood vessel construction.Methods Canine BMSCs were isolated by density gradient centrifugation and cultivated in DMEM supplemented with PDGF-BB and vitamin C(VIT-C).The phenotypic characteristics of BMSCs were identified by morphological observation,?-SMA and SMMHC in SMCs at passage 4-6 were analyzed by immunofluorescent staining,and the positive rates of SMCs at passage 5,6 were detected by flow cytometry.Results The BMSCs cultivated in the conditioned medium for SMCs showed SMC-like morphology:they displayed spindle-shape in morphology and were positive for ?-SMA but negative for SMMHC.42 d and 33 d were needed to obtain 107-108 seeding cells without or with PDGF-BB/VIT-C in culture medium respectively.With the subculture,the percentage of ?-SMA positive cells increased from 57.8% at passage 5 to 66.8% at passage 6,suggesting under aforementioned cultured conditions,more BMSCs turned into SMCs.Conclusion BMSCs can be differentiated into SMCs under appropriate culture conditions,suggesting the potentiality of using these SMCs as cell sources for tissue engineering of blood vessel construction.

Preparation and Quality Control of Compound Dexamethasone Liniment / 中国药房

OBJECTIVE:To establish a method for preparation and quality control of compound dexamethasone liniment.METHODS:The compound dexamethasone liniment were prepared with dexamethasone acetate and vitamin E,the contents were determined by HPLC.RESULTS:The calibration curves of dexamethasone acetate and vitamin E were linear in the range of2～40?g/ml and80～800?g/ml respectively,and their average recoveries were100.5%(RSD=0.82%)and98.6%(RSD=0.59%)respectively.CONCLUSION:The preparation is reasonable in formula,simple and accurate in quality control.