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Objective:To investigate the effect of miRNA (miR) -193b-5p on melanogenesis and its possible mechanisms.Methods:Human primary melanocytes were isolated from discarded normal foreskin tissues of healthy males after circumcision, and cultured in vitro. miR-NC mimics (miR-NC mimic group) and miR-193b-5p mimics (miR-193b-5p mimic group) were transfected into human primary melanocytes and human MNT1 melanoma cells, separately. After transfection, real-time quantitative PCR (RT-qPCR) was performed to determine the overexpression efficiency of miR-193b-5p at 48 hours, Western blot analysis to determine the expression of melanogenesis-related proteins tyrosinase (TYR) and microphthalmia-associated transcription factor (MITF) in human primary melanocytes and human MNT1 melanoma cells at 72 hours, and the melanin content in the above cells was determined by a sodium hydroxide solubilization method at 1 week. The target gene of miR-193b-5p was predicted by using Targetscan algorithms and verified by dual-luciferase reporter assay, and RT-qPCR and Western blot analysis were performed to analyze changes in mRNA and protein expression of the target gene respectively after the overexpression of miR-193b-5p. Two-independent-samples t test was used for comparisons between two groups. Results:In human primary melanocytes and human MNT1 melanoma cells, the miR-193b-5p expression levels were significantly higher in the miR-193b-5p mimic groups than in the miR-NC mimic groups ( t = 65.57, 22.49, respectively, both P < 0.001) , and the melanin content was significantly lower in the miR-193b-5p mimic groups (0.091 ± 0.007, 0.130 ± 0.004, respectively) than in the miR-NC mimic groups (0.117 ± 0.002, 0.188 ± 0.032, t = 5.98, 3.24, P < 0.01, < 0.05, respectively) . Western blot analysis showed that the expression of melanogenesis-related proteins TYR and MITF in both human primary melanocytes and human MNT1 melanoma cells was significantly lower in the miR-193b-5p mimic groups than in the miR-NC mimic groups (all P < 0.01) . TargetScan analysis and dual-luciferase reporter assay revealed a binding site for miR-193b-5p in the 3′ untranslated region of the transcriptional regulator CITED2. After up-regulation of miR-193b-5p expression in human primary melanocytes and human MNT1 melanoma cells, the CITED2 mRNA and protein expression levels significantly decreased compared with the miR-NC mimic groups (all P < 0.05) . Conclusion:miR-193b-5p overexpression can down-regulate the expression of melanogenesis-related proteins TYR and MITF, and then inhibit melanogenesis, which may be related to the targeted inhibition of CITED2 expression.
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Objective To evaluate the effectiveness of wechat-based transitional care in patients with Myasthenia gravis. Methods Choose 60 patients with Myasthenia gravis from August 2013 to August 2014 were divided into two groups,30 cases in each group.The patients in the two groups recevied routine dischared education.In addition,the patients in the experimental group received wechat-based transitional care for three months. The patients, anxiety, depression, self-care and health behavior at 3 months after discharge were compared between the two groups. Results There was no statistical significance between the experimental group and the control group in anxiety, depression, self-care ability and health behavior scale (P > 0.05). In the experimental group, the SAS evaluation results of normal, mild anxiety, moderate anxiety, severe anxiety respectively is 8、19、2、1. However, in the control group the result is 3、15、8、4 respectively. There was statistically difference (χ2=2.732, P < 0.01). In the experimental group, the SDS evaluation results of normal, mild depression, moderate depression, major depression respectively is 9、15、5、11. However, in the control group the result is 3、13、8、6 respectively. There was significant difference (χ2=2.626, P<0.01). Patients self-care ability and health behavior scale assessment results in the experimental group is (134.2±14.1)、(151.9±14.3) respectively and the results in the control group is (123.3±18.8)、(142.8±17.5) respectively. There was a statistically significant difference (t=2.541、2.294, P < 0.05). Conlusion Wechat-based transitional care acieves good effectiveness in patients with Myasthenia gravis and is worthy of promotion.
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Objective To evaluate the effect of family interventions on the prevention of falls in elderly hypertensive patients. Methods One hundred elderly hypertensive patients were divided into the experiment group and the control group in equal number. The control group returned for regular visits after discharge while the experiment group received the family intervention including cognitive,psychological,behavioral and environmental intervention.The two groups were compared in terms of fall rate and degree of injury.Results The incidence of falls in the experiment group was significantly lower than that of the control group,the incidence of soft tissue injury after a fall in the experiment group was significantly lower than that of the control group(both P<0.05).Conclusion Family intervention is effective in prevention of falls in elderly hypertensive patients for it may reduce the incidence of falls and the degree of fall injuries.