Pseudomonas aeruginosa-induced mitochondrial dysfunction inhibits proinflammatory cytokine secretion and enhances cytotoxicity in mouse macrophages in a reactive oxygen species (ROS)-dependent way / 浙江大学学报（英文版）（B辑：生物医学和生物技术）

随着铜绿假单胞菌（铜绿）的耐药性逐年增强，铜绿感染已经成为公共医疗卫生的重点关注问题。线粒体自噬及其介导的线粒体功能障碍在多种细菌感染中已被报道，但线粒体功能障碍在宿主调控铜绿感染中的作用尚不明确。因此，本研究建立铜绿刺激小鼠巨噬细胞感染模型和小鼠急性铜绿感染模型，探讨铜绿是否通过诱导线粒体自噬改变线粒体功能，进而影响宿主免疫炎症反应和细胞毒性，并通过监测生存率和肺组织病理学变化进一步确定线粒体自噬在小鼠铜绿体内感染模型中的作用。结果表明，铜绿引起小鼠腹腔巨噬细胞线粒体功能障碍，并通过线粒体自噬途径清除铜绿刺激引起的活性氧（ROS）累积，从而抑制铜绿引起的促炎性细胞因子分泌并增强细胞毒性。体内实验进一步确认线粒体自噬在铜绿体内感染中的作用。

The relationship between left ventricular myocardial strain and different stages of cardiac dysfunction in spontaneously hypertensive rats evaluated by two‐dimensional strain echocardiography / 中华超声影像学杂志

Objective To investigate the relationship between multi‐dimensional myocardial strain and global cardiac function in different stages of cardiac dysfunction in spontaneously hypertensive rats ( SHR ) by two‐dimensional strain echocardiography . MethodsAccording to cardiac function measurements ,SHR of 28 to 102 weeks were divided into 3 groups :Normal group[ Group A , normal left ventricular ejection fraction( LVEF) and left ventricular end‐diastolic pressure( LVEDP) , n ＝13] ,diastolic dysfunction group ( Group B , normal LVEF but increased LVEDP , n ＝24) ,and systolic dysfunction group ( Group C ,decreased LVEF and increased LVEDP , n ＝ 17 ) ,with WKY rats at similar weeks of age as controls ( group a , n ＝ 7 ;group b , n ＝ 12 ; and group c , n ＝ 16 ) . Morphological parameters of left ventricular were measured by echocardiography . Using EchoPac workstation ,systolic peak longitudinal strain ,circumferential and radial strain were calculated at the left ventricular middle levels . Extracellular collagen content was observed histologically . Results Left atrial dimension increased in group B and larger in group C ,and dilated left ventricular and thickened wall were only found in group C .Systolic peak longitudinal strain of group B was significantly lower than group A and group a ( all P ＜ 0 .05 ) ,and deteriorated in group C( P ＜ 0 .05 ) ,while systolic peak circumferential and radial strain and LVEF were only significantly decreased in group C ( all P＜ 0 .05 ) ,w hile there was no significant difference between Group A and Group B( all P ＞0 .05) . Collagen content in endocardial and mid‐layer myocardium increased in group B and C , and increased epicardial collagen occurred in group C . Systolic peak longitudinal strain , circumferential and radial strain were correlated positively with LVEF( r ＝0 .65 ,0 .80 ,0 .80 ,all P ＜0 .01) . Conclusions In SHR ,systolic peak longitudinal strain obtained by echocardiography is decreased in the period of diastolic dysfunction ,w hile the damage of systolic peak circumferential and radial strain leads to the systolic dysfunction .

The relationship between left ventricular myocardial strain and different stages of cardiac dysfunction in spontaneously hypertensive rats evaluated by two-dimensional strain echocardiography / 中华超声影像学杂志

Objective@#To investigate the relationship between multi-dimensional myocardial strain and global cardiac function in different stages of cardiac dysfunction in spontaneously hypertensive rats (SHR) by two-dimensional strain echocardiography.@*Methods@#According to cardiac function measurements, SHR of 28 to 102 weeks were divided into 3 groups: Normal group[Group A, normal left ventricular ejection fraction(LVEF) and left ventricular end-diastolic pressure(LVEDP), n=13], diastolic dysfunction group (Group B, normal LVEF but increased LVEDP, n=24), and systolic dysfunction group (Group C, decreased LVEF and increased LVEDP, n=17), with WKY rats at similar weeks of age as controls (group a, n=7; group b, n=12; and group c, n=16). Morphological parameters of left ventricular were measured by echocardiography. Using EchoPac workstation, systolic peak longitudinal strain, circumferential and radial strain were calculated at the left ventricular middle levels. Extracellular collagen content was observed histologically.@*Results@#Left atrial dimension increased in group B and larger in group C, and dilated left ventricular and thickened wall were only found in group C. Systolic peak longitudinal strain of group B was significantly lower than group A and group a(all P<0.05), and deteriorated in group C(P<0.05), while systolic peak circumferential and radial strain and LVEF were only significantly decreased in group C (all P<0.05), while there was no significant difference between Group A and Group B(all P>0.05). Collagen content in endocardial and mid-layer myocardium increased in group B and C, and increased epicardial collagen occurred in group C. Systolic peak longitudinal strain, circumferential and radial strain were correlated positively with LVEF(r=0.65, 0.80, 0.80, all P<0.01).@*Conclusions@#In SHR, systolic peak longitudinal strain obtained by echocardiography is decreased in the period of diastolic dysfunction, while the damage of systolic peak circumferential and radial strain leads to the systolic dysfunction.

Establishment and assessment of a facial nerve ischemic model in rats via the mastoid process approach / 中华显微外科杂志

Objective To estabhsh an ischemic model of intratemporal facial nerve (IFN) via the mastoid process approach.Methods From February,2015 to December,2015,45 SD rats were divided randomly into an operation group (n=35) and a shame group (n=10) in random,the right side facial nerve was used for the operation and the left side served as the control in both groups.Establish the IFN ischemia model by interrupting the petrosal artery through the mastoid process approach.Facial nerve function were evaluated at the 12h and everyday postoperatively for 28 days.The degree of IFN swelling were studied by taking paraffin sections of the decalcified temporal bone containing the IFN instantly and at the 1st,3rd,7th,14th and 21st days postoperatively.Then calculated the ratio of the cross-sectional area of the IFN and the facial canal (FN/FC).The data of behavior assessment and FC/FN were analyzed using ANOVA.Twenty-eight days after the insult,took continuous sections of brainstem containing facial nucleus,then counted the number of the facial neurons.At last,analysed the results of both operation and control sides in each group by using the student-t test.Results Facial nerve paralysis developed at 12 hour after surgery,then continued deteriorated till the 7th day.By the 28th day postoperatively,all rats in surgery group recovered and data showed no significance statistically when compared with the shame group (P＜0.05).From the value of FN/FC in different groups,the nerve were found swelling in the facial canal was increasing from the 1st postoperatively and reach the peak value at the 7th day after surgery.By the 21st day,the FN/FC come to steady but remain significant statistically when compared with the contralateral side(P＜0.05).In the operation group,facial neurons of injury side exhibited significantly loss [(41.5±3.8)％] when compared with the shame group [(98.1±4.0)％](P＜0.05).Conclusion Rats with petrosal artery interrupted exhibited significant deficits.This approach involved less tissue injury,studies on the mechanisms and therapy could become more reliable using this approach.

Ultrasonic characteristics of femoral artery atherosclerotic plaques in patients with different types of coronary heart disease / 中国医学影像技术

Objective To explore correlation between ultrasonic characteristics of femoral artery atherosclerotic plaques and non-ST-elevation acute coronary syndrome (NSTE-ACS).Methods Seventy-two patients with coronary heart disease (CHD) coexisting carotid artery and femoral artery plaques were divided into NSTE-ACS group (n=42) and chronic ischemic syndrome (CIS) group (n=30).The enhanced intensity (EI),volume,shape and internal echo level (EL) of plaques were detected with contrast-enhanced ultrasonic imaging and three-dimensional ultrasound combined with ultrasonic greyscale intensity quantitative analysis,and all parameters were analyzed between the two groups.Results EI and the proportion of irregular artery plaques were higher,and EL was lower in NSTE-ACS group than those in CIS group (all P＜0.05).EI,EL and shape of carotid artery and femoral artery plaques were correlated with NSTE-ACS (all P＜0.05).EI and EL of femoral artery plaques were risk factors for NSTE-ACS (OR=1.222,1.177,P＜0.05).Areas under ROC curve of EI and EL of carotid artery plaques were 0.801 and 0.757 (both P＜0.001),and those of femoral artery plaques were 0.814 and 0.774,respectively (both P＜0.001).Conclusion Neovascularization,shape and internal echo are correlated with NSTE-ACS,and the correlation of femoral artery plaques with NSTE-ACS is more significant than that of carotid artery plaques.Detecting ultrasonic characteristics of femoral artery atherosclerotic plaque can provide references to early identify unstable plaque and screening high-risk patients with CHD.

Correlation Between Neovascularization in Femoral Artery Plaque and Cerebral Infarction Detected by Contrast Enhanced Ultrasound / 中国医学影像学杂志

Purpose To evaluate the correlation between neovascularization in femoral artery plaque and cerebral infarction by using contrast enhanced ultrasound,and to assess the predictive value of femoral artery plaque stability in cerebral infarction and its clinical significance.Materials and Methods Sixty-two patients with femoral artery hypoechoic plaques were retrospectively studied.The patients were divided into cerebral infarction group and control group,and all of them underwent two-dimensional ultrasound and contrast enhanced ultrasound.The parameters such as the maximum thickness,the stenosis rate of cross-sectional area,the enhanced intensity (EI),the area under the curve (AUC) and the grading of the neovascularization in the plaque were detected and compared.Results The EI and AUC of the cerebral infarction group were higher than those of the control group,the differences were statistically significant (P＜0.05).Many grade Ⅲ and Ⅳ neovascularizations were found in cerebral infarction group,while the grade Ⅰ and Ⅱ neovascularizations were mostly in the control group,the difference between the two groups was statistically significant (P＜0.05).There were no significant differences in the maximum thickness and stenosis rate of cross-sectional area between the two groups (P＞0.05).The neovascularization grade,EI and AUC in the plaque were positively correlated with cerebral infarction (r=0.331,0.416 and 0.410,P＜0.05).Conclusion ① The chara-cteristics of femoral artery hypoechoic plaques in patients with cerebral infarction are rich of neovascularization and instable by using contrast-enhanced ultrasonic imaging.② The neovascularization of femoral artery hypoechoic plaque has positive correlation with cerebral infarction,which may provide a new method for clinical prediction and prevention of intracranial atherosclerosis.

Morphology changes of ocular anterior segment and its influencing factors following LASIK / 中华实验眼科杂志

Background Laser in situ keratomileusis (LASIK) is widely applicated to correct the refractive error in ophthalmology nowadays.However,the morphology and structure changes of anterior eye segment caused by the ablation of corneal stroma and suction of negative pressure during surgery should be concerned.Objective This study was to assess the changes of the anterior eye segment parameters and the factors influencing these parameters after LASIK.Methods The clinical data of 59 eyes of 31 patients who received LASIK in Chongqing Medal Eye Institute during May 2012 to July 2013 were retrospectively analyzed.The mean age of the patients was (24.52±8.41) years and the spherical equivalent was (-5.96±3.75) D.The central anterior chamber angle (ACA),anterior chamber volume (ACV),anterior chamber depth (ACD) were measured with Sirius Scheimpflug tomography,and lens thickness (LT) was measured by A-scan before operation and 1,3,6 months after operation.The anterior chamber was divided into anterior and posterior parts by the plane of cornea 4 mm radius area.The ACD of cornea 4 mm radius area sagittal height section (Sag4mm ACD) (the height from posterior corneal vertex to corneal radius of 4 mm plane) and residual ACD (RACD) (the height from corneal radius of 4 mm plane to the anterior lens surface) were computed.The association between ACD and RACD or LT were assessed by Pearson correlation analysis.Results The ACA,ACV,ACD,RACD and LT were significantly different among various time points before and after LASIK (F =8.319,P＜0.05;F =11.596,P＜0.05;F =24.045,P＜0.01;F =16.087,P＜0.05;F =15.333,P＜0.01),and the ACA,ACV,ACD and RACD were significantly decreased 1-6 months after LASIK than those before LASIK (all at P＜0.05).There were no significant differences in the Sag4 mm ACD between pre-and postLASIK (all at P＞0.05).The LT was significantly increased after surgery in comparison with that before surgery (P＜0.01).The positive correlations were found between RACD and ACD before LASIK and in 1,3,6 months after LASIK (r =0.976,0.824,0.724,0.938,all at P＜0.05) and negative correlation between LT and ACD in 3 and 6 months after LASIK (r=-0.344,P＜0.01;r =-0.363,P＜0.01).Conclusions The ACD decrease following LASIK mainly in the sector from the plane of cornea 4 mm radius area to lens surface.The change of ACD might be associated with forward shifting of anterior lens surface caused by enhanced accommodation.

An analysis on importance of drugs in traditional Chinese medicine for treatment of complications and symptoms of hypertension by QUEST algorithms / 中国中西医结合急救杂志

Objective To approach Professor Zhu Guangli's regular pattern of differentiation of symptoms and signs in patients with hypertension accompanied by complications and in accord with the individual manifestations to add or subtract the ingredients in the prescription.Methods The patients with essential hypertension selected were those who the first time came to the Affiliated Guangxing Hospital of Zhejiang Chinese Medical University or Hangzhou Hospital of Traditional Chinese Medicine (TCM) of Zhejiang Province from September 2012 to January 2015, and were continuously followed up in the hospitals having their clinical data comprehensive. The complications, combination diseases and symptoms of hypertension were conducted as dependent variables, while the applied Chinese medicines were acted as independent variables. QUEST algorithms were employed to analyze the different dependent variables to evaluate the importance contribution rate (ICR) of every kind of Chinese medicine.Results There were 9 types of complications or combination diseases [including hyperlipemia, coronary heart disease, cardiac arrhythmia, hyperglycemia, trace albuminuria, lacunar cerebral infarction, elevation of creatinine in blood (azotemia), liver dysfunction and cardiac dysfunction] and 39 types of symptoms obtained (including dizziness, palpitation, chest oppression, insomnia, lack of strength, soreness of the waist and knees, dry mouth, loose stool, fatigue, apontaneous sweating, headache, shortness of breath, coprostasis, fullness in the stomach, blurred vision, limb numbness, low spirit, chest pain, poor appetite, abdominal distension, excessive dreaming, tinnitus, flush of the face, neck rigidity, hectic fever, bitterness in the mouth, cough, ructus, nocturia, fremitus, stenagma, pharyngalgia, heaviness in head, nocturnal sweating, frequent urination, coughing of sputum, chillness and cold limbs, aphthous stomatitis and sialosis) being the QUEST decision models. All kinds of the TCM employed in all the patients paying visits for 755 times were summed up, and the individual drug frequency of presentation in the prescription was recorded. In the study, 171 sorts of TCM and 10 620 of cumulative frequency (freq.) were involved in 755 prescriptions. The average sorts of TCM was 14.07±1.53 per prescription. The top ten kinds of TCM the most commonly used (freq.) were as follows: tuckahoe (588), Chinese yam (551), wolfberry fruit (496), white peony root (442), abalone shell (405), gastrodia tuber (395), lilyturf root (325), Huai wheat (293), curcuma root (281), rush pith (277), accounting for 38.16% in the total frequency. There were 33 kinds of TCM with frequency > 100, accounting for 74.65% in the total frequency. By the analyses of QUEST algorithms directing to the complications and combination diseases to add or subtract the items of TCM, it was discovered that the drugs with ICR > 5% were as follows: in lacunar cerebral infarction, the drug application frequencies or ICRs of batryticated silkworm and bitter cardamom were 32.69% and 50.74% respectively; in coronary heart disease, the ICRs of pseudostellaria root, astragalus root, white sandal wood and lepidium seed were 23.05%, 17.70%, 15.31% and 5.06% respectively; in cardiac arrhythmia, the ICRs of astragalus root, flavescent sophora root, Huai wheat and dragon tooth and dragon bone were 46.65%, 11.56%, 7.56%, 7.13% and 6.28% respectively; in cardiac dysfunction, the ICRs of lepidium seed and white hyaciath bean were 92.97% and 7.03%; in hyperlipemia, the ICRs of cassia seed, white sandal wood, dried rehmannia root and sargentgloryvine stem were 24.26%, 12.47%, 10.51% and 5.81% respectively; in hyperglycemia, the ICRs of trichosanthes root and dried hawthorn fruit were 51.02% and 30.18%; in trace albuminuria, the ICR of snow in June herb was 83.33%; in elevation of creatinine in blood, the ICRs of Chinese actinidia root, herb of snow in June, glossy privet fruit and centella were 81.37%, 6.21%, 6.21% and 6.21% respectively; in liver dysfunction, the ICRs of coral ardisia root, purse grass and herb of stringy stonecrop were 86.37%, 6.82% and 6.82% respectively. When the symptoms were used as the dependent variables, for the top 3 commonly seen symptoms, in the prescription, the items of TCM used with ICR > 5% were as follows: in cases with elevation of blood pressure, parasitic loranthus (ICR 89.59%) was used; with dizziness, gastrodia tuber (ICR 46.96%) and/or abalone shell (ICR 33.61%) were added; headache, rhizome of Chinese ligusticum and/or rhizome of Sichuan lovage and/or large gentian with ICR at 85.77%, 7.11% and 7.11% respectively. Conclusion Professor Zhu's essential thought in differentiation of disease and expectant treatment of hypertension in accord with the individual manifestations to add and subtract drugs in TCM is under the guidance of TCM principle of differentiation of symptoms and signs for treatment, and based on the classical theories in TCM, the reliable drugs whose actions are demonstrated clinically and experimentally are preferentially selected.

Contamination status of dental unit waterlines in three general hospitals / 中国感染控制杂志

Objective To realize the contamination status of dental unit waterlines (DUWL)in general hospitals, and provide scientific evidence for making preventive measures.Methods Three hospitals were selected for study, water source adopted by hospital A,B and C was running water,reservoir water,and filtered water through reverse osmosis filtration system respectively,specimens of dental handpiece spray water and flushing water of dental chair units were collected quarterly,total bacterial colony in water were detected.Results The qualified rate of source wa-ter,handpiece spray water,and flushing water in hospital A was 75.00%(3/4),0 (0/40)and 0 (0/40)respectively,col-ony count of handpiece spray water and flushing water was (1.20×103 -5.53×104 )CFU/mL(M=3.80×104 CFU/mL) and (2.11×104 -1.66×105 )CFU/mL(M=4.80×104 CFU/mL)respectively.The qualified rate of source water,hand-piece spray water,and flushing water in hospital B was 50.00%(2/4),60.00%(24/40)and 72.50%(29/40)respectively, colony count of handpiece spray water and flushing water was (0.00 -3.71 ×106 )CFU/mL(M=83.00 CFU/mL)and (0.00-2.39×106 )CFU/mL(M=72.00 CFU/mL)respectively.The qualified rate of source water,handpiece spray wa-ter,and flushing water in hospital C was 100.00%(4/4),55.00%(22/40)and 65.00%(26/40)respectively,colony count of handpiece spray water and flushing water was (0.00-6.20×103 )CFU/mL(M=96.00 CFU/mL)and(0.00-1.63×103 )CFU/mL(M=87.50 CFU/mL)respectively.Conclusion Water of DUWL in general hospitals is seriously con-taminated,disinfection and standardized management of source water and DUWL must be strengthened.

Effect of ultrasonic coupling agent on iodine disinfectant / 中国感染控制杂志

Objective To investigate whether ultrasonic coupling agent (UCA)can produce shielding or antago-nistic effect on iodine disinfectant for preoperative skin disinfection.Methods Shielding or antagonistic effect of UCA on iodine disinfectant were detected by laboratory carrier immersion killing test and on-the-spot skin disinfec-tion test.Results Antagonistic effect:after the mixing of iodophor with UCA,the average killing rate of iodophor containing available iodine 2 500mg/L and 625 mg/L to Staphylococcus aureus decreased from 100.00% to 99.67%-99.78% and 96.85 % - 98.25 %,respectively;the average killing rate to Escherichia coli decreased from 100.00% to 99.71 %-99.82% and 95 .93 %-98.56%,respectively.Shielding effect:after smearing with UCA, the average killing rate of iodophor and iodine tincture + alcohol to Escherichia coli decreased from 100.00% to 30.76% and 100.00% to 94.48%,respectively;the average killing rate to Staphylococcus aureus decreased from 99.99% to 55 .55 % and 100.00% to 98.22%,respectively.On-the-spot skin disinfection test:the killing rate of io-dophor and iodine tincture +alcohol to natural bacteria on skin surface were both 99.99%,after skin was smeared with UCA,the killing rate decreased to 92.62% and 93 .57%,respectively.Conclusion UCA remained on the oper-ative field has shielding and antagonistic effect on iodine disinfectant.

Comparative study on the penetration abilities of resin infiltration into proximal initial caries lesions in primary molars and permanent posterior teeth / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To compare the penetration abilities of resin infiltration into proximal lesions in primary molars and permanent posterior teeth in vitro.</p><p><b>METHODS</b>Extracted or exfoliated primary molars (n = 32) and permanent posterior teeth (n = 36) with proximal white spot lesions were selected. Roots of the teeth were removed, and the crowns were cut across the caries lesions perpendicular to the surface. Cut surfaces were examined with stereo microscopy and classified with respect to histological lesion extension (C1-C4): Lesions confined to the outer half on enamel (C1), lesions confined to the inner half on enamel(C2), lesions confined to the outer half on dentin(C3), lesions extending into the inner half of dentin (C4). C4 were excluded. Caries lesions were etched for 2 min with 15% hydrochloric acid gel and were subsequently treated for 5 min with resin infiltration. Thin sections from each lesion were prepared and specimens were observed with confocal laser scanning microscopy (CLSM) through a dual-fluorescence staining technique. Lesion depth (LD) and penetration depth (PD) of resin infiltration were measured in primary molars and permanent posterior teeth in confocal microscopic images, and percentage penetration (PP) were calculated respectively. The date were collected and analyzed.</p><p><b>RESULTS</b>There were no significant differences for overall PP in primary molars and permanent posterior teeth groups (P > 0.05). However, the penetration abilities in primary molars were slight higher than those in permanent posterior teeth in C1 group (P < 0.05). The penetration abilities had no significant difference in primary molars and permanent posterior teeth in both C2 and C3 groups (P > 0.05).</p><p><b>CONCLUSION</b>Resin infiltration have satisfied penetration abilities in primary molars and permanent posterior teeth with proximal initial lesions. The better penetration abilities of resin infiltration in primary molars are shown in Cl lesions than those in permanent posterior teeth.</p>

Feasibility study of type Ⅰ hysterectomy in stage Ⅰ A cervical cancer / 肿瘤研究与临床

Objective To compare the efficacy and complications of the two surgical methods (between type Ⅰ hysterectomy and type Ⅱ hysterectomy),and to explore the feasibility of type Ⅰ hysterectomy in stage ⅠA cervical cancer.Methods The study group,92 cases(48 cases of stage ⅠA1,44 cases of stage ⅠA2) were performed with type Ⅰ hysterectomy plus selective pelvic lymph node dissection;the control group,93 cases (49 cases of stage ⅠA1,44 cases of ⅠA2) were performed with type Ⅱ hysterectomy plus selective pelvic lymph node dissection.Results The survival rate of 5 years and 10 years in study group were 100 ％ (92/92),100 ％ (74/74) and that in control group were 100 ％ (93/93),100 ％(66/66),respectively.There were no signicant difference between the two group (both P ＞ 0.05).When compared with the control group,the urinary tract infection of the study group was significantly reduced (0 versus 13.99 ％,P ＜ 0.05).Moreover,there were a shorter surgical duration [(96.14±17.20) min vs (116.82±16.30) min].The hemorrhage [(117.35±39.61) ml] and blood transfusion (0 ml) in study group was less common than those in control group [(201.74±46.25) ml,(82.07±16.32) ml] (all P ＜ 0.01).Conclusion There are no difference of 5-year and l0-year survival rate in stage ⅠA patients with type Ⅰ or type Ⅱ hysterectomy,however,the rate of the postoperative urinary tract infection in the former is lower than that in the latter,and also there are a shorter surgical duration,less hemorrhage and reduced blood transfusion requirements in study group.Therefore,type Ⅰ hysterectomy can be effective and applicable for the patients of stage ⅠA cervical cancer.

A study on the penetration abilities of natural initial caries lesions with resin infiltration / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To compare the penetration abilities of resin infiltration into natural initial caries lesions with those of adhesive in vitro.</p><p><b>METHODS</b>Thirty-six extracted human molars and premolars showing proximal white spot lesions were selected. Teeth roots were removed, and the crowns were cut across the caries lesions perpendicular to the surface. Corresponding lesion halves were etched for 2 min with 15% hydrochloric acid gel and were subsequently treated with either adhesive or resin infiltration. Specimens were observed with confocal laser scanning microscopy (CLSM) in dual fluorescence mode. In confocal microscopic images, penetration depth (PD) and lesion depth (LD) were defined as the distance from the surface to the deepest point of red and green fluorescence, respectively. The penetration percentages (PP) were calculated.</p><p><b>RESULTS</b>At the same level of caries, mean maximum lesion LD were comparable for both lesion halves (P > 0.05). But mean maximum PD and PP of the resin infiltration were significantly higher than those of the adhesive (P < 0.01).</p><p><b>CONCLUSION</b>Penetration of enamel caries lesions is observed in the adhesive and the resin infiltration. But the resin infiltration is capable of penetrating almost completely into enamel parts of natural caries lesions.</p>

Clinical report of the modified Piver class Ⅲ hysterectomy on invasive cervical cancer / 中华妇产科杂志

Objective To explore the surgical extent and to improve the surgical techniques of the Piver class Ⅲ hysterectomy on invasivc cervical cancer,so as to reduce the urinary tract complications,shorten the surgical duration,decrease the hemorrhage and blood transfusion.Methods The study group,196 cases with stages Ⅰ b and Ⅱ a carcinoma of the cervix underwent the modified Piver class Ⅲ hysterectomy from June 2000 to May 2005.The control group,176 cases of the same stages underwent the Pivet class Ⅲ hysterectomy between June 1994 and May 1999.The modified Piver class Ⅲ hysterectomy mainly include the surgical extent and some surgical techniques as follows.The cervicovesical and vesicovaginal space are separated with assistance of electrotome.Half of the uterosacral ligaments are removed with electrotome.The tunnel of the ureters is separated and penetrated or not. The anterior leaf of the cervicovesical ligaments is removed and the uterine artery are removed at the same time.while the ureter branch from the uterine artery are preserved.When the ureters aIe drawn to the lateral side of the body with an "S" hook and the urocyst lateral recessus are expanded.the cardinal ligaments can be exposed and be removed of 3/4.But part of the inferior of these ligaments should be preserved.The paracolpium are resected about 2 cm.2-3 cm tissue of the vagina is removed.Results Compare with the control group,the urinary tract complications of the study group were significantly reduced(51.1%versus 23.0%,P<0.01).There were a shorter surgical duration[(132±20)min],less of the hemorrhage[(322±100) ml]and blood transfusion[(154±79)ml] in the study group than those in the control group(all P<0.05).While,there was no significant difference at the survival rates of 5 years between the two groups (87.8% versus 88.6%.P=0.793).Conclusion The modified Piver class Ⅲ hysterectomy is effective and applicable for patients with cervical cancer.

The influence of the PBSC collection yield by choosing the different venous accesses in the healthy donors / 中国实用护理杂志

Objective To investigate the influence of the PBSC collection yield by choosing the differ-era venous accesses in the healthy donors. Methods 118 healthy PBSC donors performing PBSC collection between January 2000 and December 2007 in our hospital were divided into four groups according to the differ-ent venous accesses. The PBSC collection yield of four groups,including mononuclear cells (MNC) count and CD34+ cells count were observed. Results In the ulnar V-ulnar V group,MNC (5.31±2.29)×108/kg,CD34+ cells (4.78±2.06)×106/kg;ulnar V- antecubital V group,MNC(5.11±2.34)×108/kg,CD34+cells(4.34±1.99)×106/kg;antecubital V- antecubital V group,MNC (5.61±1.73)±×108/kg,CD34+cells (4.60±1.42)×106/kg;ulnar V- radial V group,MNC(4.60±×1.70)×108/kg,CD34+cells (4.05±1.50)×106/kg.There was no statistical differ-ence of the PBSC collection yield between four groups (P>0.05). Conclusions Different venous accesses don't affect the PBSC collection yield in the PBSC healthy donors.

The study of performing therapeutic leukapheresis and plateletpheresis sequencially with a single apheresis kit / 中国实用内科杂志

0.05).Conclusion Performing leukapheresis and plateletpheresis sequencially with a single Apheresis Kit is a safe,effective,practicable and economic method in the treatment of chronic myeloid leukemia with leukocytosis and thrombocytosis.It can drop the medical expanse markedly.

The Accessing Care of The Early Health Interference and The Growth of Infant Times on Premature Very Low-Birth-Weight-Infant / 中华围产医学杂志

Objective To investigate the relationship factor of livability on Premature Very Low-Birth-Weight-Infant (PVLBWL), for refer to evidences that reduce the mortality and the deformity rate. Methods The group 116 PVLBWL cases, being in our hospital hear five years, divide into three team according to the weight, and analyze the review clinical data of the cases (from 1993.1 to 1998.1). Results There are 86 cases that had cured and left hospital in the group. The cure rate is 74.1%(86/116). It is rise along with the weight increase (P

Study of oxidative stress and inflammatory reaction in preeclampsia / 中华围产医学杂志

0.05,respectively).Conclusions Oxidative stress and inflammatory reaction play an important role in the pathogenesis of PE.Antioxidant and anti-inflammatory treatment may prevent the onset and progress of PE.

Study on the reversion of drug resistance in human cervical cancer cell lines / 中华妇产科杂志

Objective To determine the resistance reversion of mitomycin (MMC) by 3′-Keto-bmt 1-val 2-cyclosporin (SDZ PSC 833) in human cervical cancer in vitro and in vivo. Metheds A xenografted mitomycin resistant mice model of cervical cancer was devolped. The reversion of mitomycin resistance by SDZ PSC 833 (1 or 3 mg/L) was detected from human cervical cancer cell (Hela) and its resistant subline Hela/MMC in vitro and in vivo. Studies in vitro include drug resistance reversion experiment and the changes of morphology. Studies in vivo including tumor volume and tumor related histopathological changes in the autopsied specimen were evaluated by comparing random sections of each group. Results Nontoxic doses of SDZ PSC 833 could result in almost partial reversion of MMC-resistance of Hela/MMC.In vivo studies also showed SDZ PSC 833 augmented the growth inhibitory effect of mitomycin on Hela/MMC xenografted in nude mice. Conclusion SDZ PSC 833 can overcome mitomycin resistance of Hela/MMC in vitro and in vivo ,so SDZ PSC 833 will be a better candidate clinically for reversing multidrug resistance.

Diagnosis and treatment of recurrent epithelial ovarian carcinoma / 中华妇产科杂志

Objective To investigate the diagnosis,treatment and prognosis of recurrent epithelial ovarian carcinoma. Methods Fifty-two patients with recurrent epithelial ovarian carcinoma who were admitted in Zhongnan Hospital of Wuhan University between 1997 and 2002 were analyzed retrospectively,and their diagnostic methods and the survival rates were compared. Results The positive diagnostic rate of pelvic examination,CA 125 and ultrasound examination was 73.1%,84.6% and 53.8%,respectively to the recurrent epithelial ovarian carcinoma. The median survival time of group A(surgery+chemotherapy) and group B ( chemotherapy) was 11 and 12 months,respectively. Conclusions The diagnostic methods should include pelvic examination,CA 125 and ultrasound examination. We could not know the exact role of surgery to the management and prognosis of recurrent epithelial ovarian carcinoma. Chemotherapy may have an important role to the management and prognosis of recurrent epithelial ovarian carcinoma.