Camganoids A and B, two new sesquiterpenes with different carbon skeletons isolated from fruits of Cinnamomum migao / 中草药·英文版

Objective: To isolate and identify the undescribed compounds from the fruits of Cinnamomum migao and evaluate its nitric oxide inhibition potential. Methods: The chromatographic techniques of silica gel, Sephadex, and HPLC were used for isolation and purification of the compounds, while HR-ESI-MS, 1D NMR, 2D NMR, ECD, and X-ray diffraction techniques were used to characterize and confirm the isolated compounds. Moreover, the anti-inflammatory activity of the isolated compounds was carried out to check inhibitory potential against the production of nitric oxide with RAW264.7 cells stimulated by LPS. Results: Camganoid A (1), a novel sesquiterpene possessing an unprecedented skeleton, and camganoid B (2), containing a unique eight-membered sesquiterpene moiety with a new carbon skeleton, were isolated and identified from the fruits of C. migao. The absolute configurations of 1 and 2 were confirmed by single crystal X-ray diffraction and electronic circular dichroism (ECD) calculations. Among these compounds, compound 1 exhibited potent inhibitory activity against the production of nitric oxide with IC

Study on HPLC fingerprint of Ginseng Radix et Rhizoma Rubra / 药学实践杂志

Objective To establish the HPLC fingerprint of Ginseng Radix et Rhizoma Rubra for its quality control . Methods Sharpsil-T C18 column(4 .6 mm × 250 mm ,5 μm)was used with acetonitrile-water in gradient elution mode .The flow rate was 1 .0 ml/min ,the column temperature was maintained at 30 ℃ ,and the detective wavelength was 203 nm .Results 24 co-possessing peaks were selected as fingerprint peaks and the similarities between each of the ten areas and the standard chro-matographic fingerprints of Panax ginseng Rubra were calculated while Rb1 peak selected as the reference peak .The similarity was more than 0 .940 .Eleven chemical compounds were identified by comparing the reference substance .Conclusion The method could be used for the quality control of Panax ginseng Rubra with good precision ,stability ,and reproducibility .

Determination of oleanolic acid and ursolic acid in Shanzhajing Jiangzhi tablet by HPLC / 药学实践杂志

Objective To establish the quality standard for Shanzhajing Jiangzhi tablet by HPLC .Methods Samples were analyzed on a Agilent Zorbax SB C18 column(250 mm × 4 .6 mm ,5μm) with methanol and 0 .06 mol/L ammonium acetate (85∶15)as mobile phase at the flow rate of 0 .8 ml/min .The wavelength and column temperature were set at 210 nm and 25 ℃ ,respectively .Results The calibration curve showed a good linear relationship within the range of 0 .124-2 .48 μg for oleanolic acid (r=0 .999 7) and 0 .498-9 .96 μg for ursolic acid (r=0 .999 8) .The average recovery ratio of the oleanolic acid and ursolic acid were 96 .9% (RSD=1 .26% ) and 100.4% (RSD=2 .6% ) ,respectively .Conclusion The method was proved to be good at evaluation effectiveness and practicality .

Summary of the pharmacokinetic methods of traditional Chinese medicine prescription / 药学实践杂志

This review made a brief summary of several pharmacokinetic methods of traditional Chinese medicine (TCM ) prescription including the methods of biological effects ,plasma concentration ,integrated Pharmacokinetics of multiple effective components of TCM prescription and integrated pharmacokinetic‐pharmacodynamic of TCM prescription .The corresponding characteristics ,advantages and disadvantages of each of these methods were analyzed .And the progresses of the pharmacokinetic methods of TCM prescription were concluded and prospected .

Identification of Inulae Herba, Inulae Flos and Their Closely Related Species Based on ITS2 Sequence / 世界科学技术-中医药现代化

This study aimed to identify Inulae Herba, Inulae Flos and their closely related species using the ITS2 bar-code, and secure the quality and clinical curative effect of these medicinal materials. DNA was extracted from Inula linariifolia, I. japonica, I. britanica, which are the original species of Inulae Herba and Inulae Flos, together with their closely related species. The ITS2 sequence was amplified by PCR and sequenced bidirectionally. Sequence assembly and generation of consensus sequence were conducted by the CodonCode Aligner. The genetic distances were comput-ed using MEGA 5.0 in accordance with the Kimura-2-parameter (K2P) model, and the phylogenetic tree constructed by the neighbor-joining (NJ) method. The results showed that the ITS2 sequences of the various species have stable variable sites. The intraspecific genetic distances among Inulae Herba and Inulae Flos were obviously lower than the interspecific genetic distance among the above two medicinal materials and its adulterants. The NJ tree based on ITS2 sequences can clearly differ from Inulae Herba, Inulae Flos and their closely related species. It is concluded that ITS2 sequence can be used as DNA barcode to identify Inulae Herba, Inulae Flos and their closely related species.

Sesquiterpene lactones from Inula helianthus-aquatica / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the sesquiterpene lactones of the aerial parts of Inula helianthus-aquatica.</p><p><b>METHOD</b>Compounds were isolated and purified by silica gel, Sephadex LH-20 and preparative HPLC. On the basis of physicochemical properties and spectroscopic data, their structures were identified.</p><p><b>RESULT</b>Seven sesquiterpene lactones and four other compounds were obtained and identified as 2-desoxy-4-epi-pulchellin (1), 6-acetoxy-4-hydroxy-1, 10H-pseudoguaia-11 (13)-en-12,8-olide (2), 4-acetoxy-6-hydroxy-1, 10H-pseudoguaia-11(13)-en-12,8-olide (3), 8-epi-inuviscolide (4), 2,3,11,13-tetrahydroaromaticin (5), 11,13-dihydro-ergolide (6), 4-epipulchellin-2-O-acetate (7), 7-epiloliolide (8), loliolide (9), beta-sitosterol (10) and daucosterol (11).</p><p><b>CONCLUSION</b>All the compounds were isolated from this plant for the first time.</p>