RESUMO
Methicillin resistant Staphylococcus aureus [MRSA] is resistant to known antibiotics and has become a great challenge for healthcare professionals, therefore new molecules are needed to manage this situation. In this study, new lead molecules 4-Amino-5-[2-Hydroxyphenyl]-l,2,4-Triazol-3-Thione [Ul] and4-[2-hydroxybenzalidine] amine-5-[2-hydroxy] phenyl-l,2,4-triazole-3-thiol[U!A Schiff base] were synthesized by fusion method that showed promising antibacterial activity [U1A: 26mm and Ul: 14mm] against MRSA.FT-IR and NMR were used for structural characterization of these derivatives and their toxicity properties were assessed by Lipinski's rule of 5. New potential drug targets of this bacterium were also identified by comparative and subtraction genomics techniques. In particular, octanoyl-[GcvH]: protein N-octanoyl transferase and phosphor mevalonate kinase were used as potential targets in AutoDock Vina studies. This study can provide a framework to find potential drug targets for other pathogenic microorganisms that can successfully be docked with compound Ul and Ul A