Interplay of Chemo Attractant Peptides (Cathelicidin and Chemerin) with Vitamin- D in Patients with Pulmonary Tuberculosis.

Aim: Both Cathelicidin and Chemerin are chemo-attractant proteins and possess antimicrobial activity. Sufficient level of Vitamin D is important for optimum response of Cathelicidin for its antimycobacterial activity. Studies on the role of these antimicrobial peptides and their relationship with Vitamin D level are limited in tuberculosis. The aim of this study was to investigate an association of Vitamin D with antimicrobial peptide (Cathelicidin) and an adipokine (Chemerin) in patients with pulmonary tuberculosis (TB). Methods: In a case control study we estimated level of Vitamin D, Chemerin, Cathelicidin and TNF α in pulmonary TB patients (n=22) and healthy endemic controls (n=17) using sandwich ELISA methodology. The study was conducted at Aga Khan University Karachi during 2011. Results: TB group had higher proportion of subjects above median level of Cathelicidin (median test; p=0.034) and fewer number of subjects with Chemerin (median test; p=0.001). Pairwise comparison also showed significant differences between average ranks of Vitamin D vs. Cathelicidin (p<0.0001), Chemerin vs. Cathelicidin (p=0.04) and Vitamin D vs. TNFα (p<0.0001). Cathelicidin was identified as most discriminatory marker between TB disease and healthy group (ROC, AUC 0.780; p=0.007). Conclusion: Our results highlight the role of Cathelicidin as a potential biomarker of active TB disease. The role of Cathelicidin and Chemerin as plausible biomarkers requires further studies in both inflammatory and non-inflammatory conditions.

Humoral immune response to filarial antigens in chyluria.

Humoral immune parameters like total immunoglobulins and specific antibody levels in serum were studied in filarial chyluria patients. Mean serum IgG was significantly reduced in this group compared to normal controls, while IgA and IgM levels remained comparable to controls. Anti-filarial antibody titre as measured by enzyme-linked immunosorbent assay also was significantly reduced. However, the total and specific IgE antibody titre was similar to that of controls. Specific IgE contents of the patients' sera could be related to their microfilaraemic status.