Prevention of obesity using low carbohydrate ketogenic diet

This review focuses on the effect of low carbohydrate ketogenic diet on obese subjects presenting with various metabolic syndromes. Here, we provide data from our laboratory and from various other investigators on the therapeutic effectiveness of ketogenic diet on obese subjects. In this review we provide the rationale behind using ketogenic diet as a treatment of obesity and its beneficialroleinneurodegenerative / neurological disorders, diabetes, hyperlipidemia, coronary diseases, cancer etc. Administering ketogenic diet for a relatively longer period did not produce any significantsideeffect. Therefore, based on the data presented in this review, it is recommended that it is safe to use ketogenic diet for a longer period of time for obesity and associated disorders

Heat treatment of bacteria: a simple method of DNA extraction for molecular techniques

To evaluate the efficacy of two simple methods involving use of heat for extraction of bacterial deoxyribonucleic acid [DNA] be used in molecular techniques like polymerase chain reaction [PCR], restriction fragments length polymorphism [RFLP] and DNA sequencing and compare them with DNA extraction using commercial kits. DNA extraction by improved alternative methods and commercial kit. Microbiology Research Laboratory, Faculty of Allied Health Sciences, Kuwait University, Kuwait. Forty isolates of Klebsiella pneumoniae. DNA was extracted from isolates by either boiling for 10 minutes or microwave irradiation for 10 seconds. For comparison, DNA was also extracted using a commercial kit. All extracted DNA samples were analyzed by PCR, RFLP and / or DNA sequencing of TEM and SHV genes of the bacteria. Successful extraction of DNA. PCR, RFLP and DNA sequencing gave the expected results in all the DNA samples extracted by all the three methods [boiling, microwave irradiation and the commercial kit]. The results were qualitatively equivalent in all methods. Heat may be used to extract DNA from K. pneumoniae which can be utilized successfully in performing PCR, RFL and DNA sequencing