Immune reconstitution after allogeneic hematopoietic stem cell transplantation in children: a single institution study of 59 patients / 소아과

PURPOSE: Lymphocyte subset recovery is an important factor that determines the success of hematopoietic stem cell transplantation (HSCT). Temporal differences in the recovery of lymphocyte subsets and the factors influencing this recovery are important variables that affect a patient's post-transplant immune reconstitution, and therefore require investigation. METHODS: The time taken to achieve lymphocyte subset recovery and the factors influencing this recovery were investigated in 59 children who had undergone HSCT at the Department of Pediatrics, The Catholic University of Korea Seoul St. Mary's Hospital, and who had an uneventful follow-up period of at least 1 year. Analyses were carried out at 3 and 12 months post-transplant. An additional study was performed 1 month post-transplant to evaluate natural killer (NK) cell recovery. The impact of pre- and post-transplant variables, including diagnosis of Epstein-Barr virus (EBV) DNAemia posttransplant, on lymphocyte recovery was evaluated. RESULTS: The lymphocyte subsets recovered in the following order: NK cells, cytotoxic T cells, B cells, and helper T cells. At 1 month post-transplant, acute graft-versus-host disease was found to contribute significantly to the delay of CD16+/56+ cell recovery. Younger patients showed delayed recovery of both CD3+/CD8+ and CD19+ cells. EBV DNAemia had a deleterious impact on the recovery of both CD3+ and CD3+/CD4+ lymphocytes at 1 year post-transplant. CONCLUSION: In our pediatric allogeneic HSCT cohort, helper T cells were the last subset to recover. Younger age and EBV DNAemia had a negative impact on the post-transplant recovery of T cells and B cells.

Acute Acalculous Cholecystitis with Bacteremia Caused by Streptococcus anginosus Following Dental Procedure in a Previously Healthy Adolescent / 소아감염

Streptococcus anginosus is a member of Streptococcus milleri group, and is found in the oral mucosa, respiratory tract, and gastrointestinal tract as normal flora. It can develop into a disease in patients with deteriorating clinical condition or with clinical risk factors. A previously healthy 15-year-old boy was admitted due to fever, abdominal discomfort and vomiting which lasted for 7 days. He had a history of dental procedure 1 day before the development of fever. He was diagnosed with acute acalculous cholecystitis based on the clinical, laboratory, and imaging finding, and S. anginosus was isolated from the blood culture. The patient was successfully treated with antibiotic therapy.

Shear bond strength of composite resin core used in combination with various resin cements and dentin bonding agents / 대한치과보철학회지

PURPOSE: The purpose of this study was aimed to compare the shear bond strength on dentin of three dentin bonding agents and two resin cements used in conjunction with self-cured composite resin core material. MATERIAL AND METHOD: Control group and six experimental groups were divided for this study. Control group was designated as specimens bonded with Tenure A & B(R). Experimental groups were as follows: PB-BL group : specimens bonded with PrimeandBond NT(R), BondLink(R). SB-BL group : specimens bonded with SingleBond(R), BondLink(R). PB group : specimens bonded with PrimeandBond NT(R) SB group : specimens bonded with SingleBond(R). PF group : specimens bonded with Panavia-F(R). BI group : specimens bonded with Bistite II(R). All specimens were stored in 37degrees C distilled water for 24 hours, followed by the shear bond strength was tested by universal testing machine. The data was analysed statistically by Mann-Whitney test. RESULTS: 1. For PrimeandBond NT(R) and SingleBond(R), the shear bond strength was 0.24 MPa and 7.19 MPa each by each, while Tenure A & B(R) group control was measured at 13.93 MPa (p<0.05). Especially for PrimeandBond NT(R), it did not get conjunction with dentin. 2. For PrimeandBond NT(R) and SingleBond(R) using BondLink(R), there was no significant difference as a result of 11.73 MPa and 14.00 MPa each by each (p<0.05). 3. For Panavia-F(R) and Bistite II(R), they showed the highest shear bond strength as measured by 18.24 MPa and 16.09 MPa each(p<0.05).