Long-Term Fate of the Anastomosed Digital Artery after Successful Replantation

We have found that some replanted fingers were survived not by the supply of blood from anastomosed arteries but by the neovascularization of the soft tissue. Therefore, we checked if the anastomosed arteries will consistently be in patency and the average time it takes for anastomosed digital arteries to be occluded after replantations. We surveyed a total of 75 fingers to evaluate patency of the anastomosed arteries using Doppler. We performed angiogram for 15 fingers on the average of 30th day after replantations. We further analyzed the causes, types, levels, vein graft, and the number of venorrhaphies. We couldn't find, using the Doppler, the pulsations of the anastomosed arteries on 28 fingers but could find the occlusions of anastomosed arteries from 10 fingers on angiogram. In case of 43 percent in crushing and 8 percent in clean cut, pulsation was not heard in the Doppler (p<0.05). The unheard ratios is 51 percent in distal phalanx, 8 percent in mid-phalanx and 37 percent in proximal phalanx, respectively. Twenty-eight fingers (37%) out of 75 didn't show consistent patency on the average of 15 days after operation. The results show that the causes and levels of amputation were factors affecting the patency of arteries.

Human Dermal Fibroblast Viability Against Time and Calcium Alginate Density in Vitro

One approach for soft tissue augmentation is tissue engineering technique which uses autogenous fibroblasts localized within a biocompatible polymer. Many reports of chondrocytes within calcium alginate for cartilage transplantation in vitro have been presented, but no report of fibroblasts within calcium alginate has been presented. This study is related to the conditions of polymerization depending on alginate density and culture time in the viability of human fibroblasts encapsulated in calcium alginate matrix. Human dermal fibroblasts were obtained from STSG patients, enzymatically dissociated, and cultured in DMEM/ Ham's F-12, then the fibroblasts were collected by centrifugation. Alginate discs containig fibroblasts were made from 1 or 2% sodium alginate mixed in 150 mM CaCl2 solution. Viability of fibroblasts was measured by quantification of the DNA content per alginate disc at six different time intervals from 1 to 8 weeks. Significant initial cell loss was observed in the first two weeks after which the survival rate remained stationary. According to the alginate density, fibroblasts seeded at 1% alginate showed higher survival rate than at 2% alginate in early periods(by 6 weeks), then inversed in late periods(p<0.05). Our study provides a significant information in manufacturing alginate-fibroblast induced soft tissue by tissue engineering.

Human Dermal Fibroblast Viability Against Time and Calcium Alginate Density in Vitro

One approach for soft tissue augmentation is tissue engineering technique which uses autogenous fibroblasts localized within a biocompatible polymer. Many reports of chondrocytes within calcium alginate for cartilage transplantation in vitro have been presented, but no report of fibroblasts within calcium alginate has been presented. This study is related to the conditions of polymerization depending on alginate density and culture time in the viability of human fibroblasts encapsulated in calcium alginate matrix. Human dermal fibroblasts were obtained from STSG patients, enzymatically dissociated, and cultured in DMEM/ Ham's F-12, then the fibroblasts were collected by centrifugation. Alginate discs containig fibroblasts were made from 1 or 2% sodium alginate mixed in 150 mM CaCl2 solution. Viability of fibroblasts was measured by quantification of the DNA content per alginate disc at six different time intervals from 1 to 8 weeks. Significant initial cell loss was observed in the first two weeks after which the survival rate remained stationary. According to the alginate density, fibroblasts seeded at 1% alginate showed higher survival rate than at 2% alginate in early periods(by 6 weeks), then inversed in late periods(p<0.05). Our study provides a significant information in manufacturing alginate-fibroblast induced soft tissue by tissue engineering.

Influences of Betadine and Hydrogen Peroxide on Vasospasm

Vasospasm influences on final results of microanastomosis such as free flap or replantation. Postoperative vasospasm was induced by multiple factors. Many patients had to be exposed to repetitive use of dressing materials (Betadine, Hydrogen peroxide), because areas that distal to the microanastomotic sites were vulnerable to infection. Spastic abilities of Betadine and Hydrogen peroxide(H2O2) had not been known. We performed experimental study for influences of Betadine and H2O2 on vasospasm after microanastomosis. Twenty Sprague-Dawley rats(300 - 345g) were randomly assigned to one of two groups, one was Betadine(n = 10) and the other was H2O2(n = 10). The femoral arteries were exposed, bilaterally. The left femoral artery was used as a test site(Betadine, H2O2), and the right as a control (normal saline). The initial arterial diameter were measured under a microscopy using vernier calipers, 20 minutes after dissection. Another 20minutes later, Betadine and H2O2, normal saline were dropped. The final arterial diameter were measured, 10 minutes after dropping. Gross examination was done during 10minutes, too. In Group I, the initial mean arterial diameters were 0.775+/-0.075 mm(Lt.), 0.785 +/- 0.041 mm(Rt.) and the final mean arterial diameters were 0.785 +/- 0.068 mm(Lt), 0.785 +/- 0.048 mm(Rt.). In Group II, the initial mean arterial diameters were 0.775 +/- 0.052 mm(Lt.), 0.780 +/- 0.040 mm (Rt.) and the final mean arterial diameters were 0.765 +/- 0.055 mm(Lt.), 0.775 +/- 0.045 mm(Rt.). There was no significant statistical difference between each materials and its control, and no changes on gross examination.We concluded that there is no influences of Betadine and H2O2 on vasospasm after microanastomosis.

Influences of Betadine and Hydrogen Peroxide on Vasospasm

Vasospasm influences on final results of microanastomosis such as free flap or replantation. Postoperative vasospasm was induced by multiple factors. Many patients had to be exposed to repetitive use of dressing materials (Betadine, Hydrogen peroxide), because areas that distal to the microanastomotic sites were vulnerable to infection. Spastic abilities of Betadine and Hydrogen peroxide(H2O2) had not been known. We performed experimental study for influences of Betadine and H2O2 on vasospasm after microanastomosis. Twenty Sprague-Dawley rats(300 - 345g) were randomly assigned to one of two groups, one was Betadine(n = 10) and the other was H2O2(n = 10). The femoral arteries were exposed, bilaterally. The left femoral artery was used as a test site(Betadine, H2O2), and the right as a control (normal saline). The initial arterial diameter were measured under a microscopy using vernier calipers, 20 minutes after dissection. Another 20minutes later, Betadine and H2O2, normal saline were dropped. The final arterial diameter were measured, 10 minutes after dropping. Gross examination was done during 10minutes, too. In Group I, the initial mean arterial diameters were 0.775+/-0.075 mm(Lt.), 0.785 +/- 0.041 mm(Rt.) and the final mean arterial diameters were 0.785 +/- 0.068 mm(Lt), 0.785 +/- 0.048 mm(Rt.). In Group II, the initial mean arterial diameters were 0.775 +/- 0.052 mm(Lt.), 0.780 +/- 0.040 mm (Rt.) and the final mean arterial diameters were 0.765 +/- 0.055 mm(Lt.), 0.775 +/- 0.045 mm(Rt.). There was no significant statistical difference between each materials and its control, and no changes on gross examination.We concluded that there is no influences of Betadine and H2O2 on vasospasm after microanastomosis.