Correlation of the Expression of nm23-H1/NDP Kinase A and CD44v8-10 with Head and Neck Cancer Metastasis / 체질인류학회지

The expressions of nm23-H1/NDP kinase and CD44 genes which are associated with tumor proliferation and metastasis, in 10 cases of head and neck cancer were studied by immunohistochemistry using monoclonal antibody H1-203 and CD44v8-10. And the relationship between the expressions of two genes and cancer metastasis was studied. Eight cases out of 10 head and neck cancers were squamous cell carcinomas. Normal skin epidermis and squamous cell carcinoma of the skin were used as control. The expression of nm23-H1/NDP kinase A was decreased generally in head and neck cancer comparing with normal epidermis, and more decreased in metastatic head and neck cancer. CD44v8-10 was shown strong expression in non-metastatic head and neck cancer but weak or no expression in metastatic head and neck cancer. With differentiation of tumor tissue, the expression of CD44v8-10 gene decreased. These results suggest that nm23-H1/NDP kinase and CD44 genes play a role as inhibitor to metastasis in head and neck cancer.

Treatment Result in Laryngeal Cancer Patients Submitted to Surgical Treatment / 대한이비인후과학회지

BACKGROUND AND OBJECTIVES: Laryngeal cancer is the most common malignant tumor within head and neck and is known for having a good prognosis in comparison to the other malignancies. The surgery to the larynx and neck is one of the prime treatment modality for the laryngeal cancer. The purpose of this study is to review the surgical experience of our hospital and to focus on the results of the surgical treatment of the laryngeal cancer. MATERIALS AND METHODS: Analyzed 41 laryngeal cancer patients who underwent surgery during the period of 5 years from January 1989 to December 1993. TNM staging of the disease was done by the AJCC staging system issued in 1992. The 5-year survival rate was calculated by Kaplan-Meier method. RESULTS: 1) The overall 5-year survival rate was 65.8%. 2) The 5-year survival rate according to clinical stage showed 100%, 83.3%, 76.9%, and 50% in stage I, II, III, IV respectively. 3) The 5-year survival rate according to T stage showed 75%, 66.7%, 57.9%, 77.8%, in T1, T2, T3, T4 respectively. 4) The 5-year survival rate according to nodal status showed N(-) 78.9%, N(+) 56.2%. 5) The 5-year survival rate according to primary site showed supraglottis 60%, glottis 100%, transglottis 57.1%. 6) The 5-year survival rate according to treatment modalities showed surgery only patients 80%, postoperative radiation patients 60%, salvage operation patients 54.6%. CONCLUSION: Early stage, glottic cancer and the patients who only had operation showed good treatment result. Regional lymph node metastasis, at the time of diagnosis, was found to carry a worse prognosis. The main cause of treatment failure was distant metastasis.

Cavernous Sinus Lesions Treated in Otorhinolaryngology-Head and Neck Surgery Field / 대한이비인후과학회지

BACKGROUND AND OBJECTIVES: Cavernous sinus is a small venous space enclosed by leaves of dura and periosteum and located on either side of sella turcica. Approach and treatment of lesions of this sinus are very difficult and perilous because they contain critical neural and vascular structures. Surgery relating to cavernous sinus lesions are extremely rare in otorhinolaryngology and such surgeries are usually referred to the fields of neurology and neurosurgery. However, in the case of a lesion that extends from the nasal cavity and the paranasal sinuses, an intranasal approach is necessary and cooperation with head and neck surgeons are desirable to achieve a superior result. This study examines the nature of these rare cases of cavernous sinus lesions and assesses the results of cooperation with doctors of other specialties. MATERIALS AND METHODS: We summarize here three cases of cavernous sinus lesions (angiofibroma, tuberculosis, foreign body) extendeding from the nasal cavity and paranasal sinuses. These lesions were treated in cooperation with a reconstructive surgeon and a neurosurgeon. RESULT: All of these cases showed successful results. CONCLUSION: Close cooperation from each department is critical in order to achieve successful results.

Cytogenetic Studies of High Dose EGF Treated KUMA3 Cell Clone / 대한이비인후과학회지

BACKGROUND AND OBJECTIVES: Epidermal growth factor (EGF), directly stimulates epidermal growth and differentiation. The combination of EGFR activation and genetic alternations may lead to neoplasia and metaplasia. To study the change of chromosomal number and the aberrations of chromosomal structure of KUMA3 cell line treated with high dose EGF. MATERIALS AND METHODS: The high dose EGF treated cell clones were obtained from KUMA3 cell line which was established from squamous cell carcinoma of the lower lip by culturing cells in medium containing high dose EGF for 6 months. The chromosomal analysis and subculture were performed at subsequent passage of 1 month interval. RESULTS: In high dose EGF treated cell clones, there was no apparent change in chromosomal number, but the ratio of the number of chromosome 7 to mode chromosome number was similar to normal value (0.043). The new chromosomal structural aberrations appeared first from 30 passage of IR-200 cell clone. The chromosomal aberrations were del(1)(q23-->qter) and del(4)t(1:4)(1qter-->1q23: : 4p16-->4qter). CONCLUSION: There was no change in chromosomal number, but the ratio of the number of chromosome 7 to mode chromosome number was similar to normal value (0.043), and the new chromosomal structural aberrations were appeared.