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Background@#: This is a methodological study for validation of the Korean version of scrub practitioners’ list of intra-operative nontechnical skills system, K-SPLINTS.Method : Data were collected from multidisciplinary expert panel (n=6) and a group of scrub nurses (n=40) with minimum two years of operative theatre experience in two university hospitals in Seoul. Contents validity was calculated from expert panel and reliability, completeness, observability and usability were analyzed from scrub nurses group.Result : K-SPLINTS is deemed to be adequate for assessing and training scrub nurses’ intraoperative behaviours. @*Conclusion@#: K-SPLINTS could be useful to inspire scrub nurses non-technical skills in the operating theatre.
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BACKGROUND: Infection by the intracellular bacteria Mycoplasma pneumoniae, Chamydophila pneumoniae, and Legionella pneumophila are common causes of community-acquired pneumonia (CAP). This study describes the evaluation of a new multiplex real-time PCR test, EuDx™-PN MLC Detection Kit (EUDIPIA), which allows the simultaneous detection of M. pneumoniae, C. pneumoniae, and L. pneumophila in respiratory samples. METHODS: A total of 353 samples were tested using three PCR kits: multiplex PCR (Seeplex PneumoBacter ACE Detection Kit) and two multiplex real-time PCR (EuDx™-PN MLC Detection Kit and Anyplex™ II RB5 Detection Kit). The results were considered true positives (expanded standard) for M. pneumoniae, C. pneumoniae, and L. pneumophila if they were positive according to any of the three tests. RESULTS: The sensitivity and specificity of EuDx™-PN MLC Detection Kit were 93.3–100% and 100%, respectively. The agreement rate and Cohen's kappa coefficient (value) between EuDx™-PN MLC Detection Kit and Anyplex™ II RB5 Detection Kit for M. pneumoniae, C. pneumoniae, and L. pneumophila were 70–100% and 0.82–1, respectively. CONCLUSION: These results demonstrate that the EuDx™-PN MLC Detection Kit is a sensitive, specific, and useful screening tool for the detection of atypical pathogens in respiratory samples and can be helpful in selecting appropriate antimicrobial therapy for patients with respiratory infection.
Assuntos
Humanos , Bactérias , Pneumonia por Clamídia , Chlamydophila pneumoniae , Chlamydophila , Legionella pneumophila , Legionella , Programas de Rastreamento , Reação em Cadeia da Polimerase Multiplex , Mycoplasma pneumoniae , Mycoplasma , Pneumonia , Pneumonia por Mycoplasma , Reação em Cadeia da Polimerase , Reação em Cadeia da Polimerase em Tempo Real , Infecções Respiratórias , Sensibilidade e EspecificidadeRESUMO
No abstract available.
Assuntos
Feminino , Humanos , Proteína BRCA1/genética , Proteína BRCA2/genética , Sequência de Bases , DNA/química , Bases de Dados Genéticas , Síndrome Hereditária de Câncer de Mama e Ovário/genética , Sequenciamento de Nucleotídeos em Larga Escala , Polimorfismo de Nucleotídeo Único , Análise de Sequência de DNARESUMO
BACKGROUND: Hepatitis C virus (HCV) RNA quantification is necessary for predicting the therapeutic response and assessing treatment results in patients with chronic HCV infection. Recently, real-time PCR technology for HCV RNA quantification displayed good linearity within the dynamic range. Thus, it is gradually replacing branched-DNA (bDNA) and PCR- hybridization assays. In this study, we evaluated the performance of the Real-QTM HCV quantification kit (biosewoom. Inc., Seoul, Korea) developed in Korea. METHODS: We evaluated the HCV quantification kit for detection limit, specificity, linearity, accuracy, and recovery rate of HCV RNA standard material. The results were analyzed for a correlation with those of Cobas Amplicor HCV Monitor 2.0. RESULTS: The HCV quantification kit showed a high recovery rate of HCV RNA standard material of various concentrations and amplication of HCV RNA equally in all genotypes. Hepatitis B virus and human immunodeficiency virus showed no cross-reactivity with HCV. Within-run and between-run coefficients of variation (CV) were 9.52~15.84% and 9.40~17.53%, respectively. Between-day coefficients of variation were 11.62~18.04%, and detection limit was 44 IU/mL. It showed a good correlation with Cobas Amplicor HCV Monitor 2.0 (R2=0.8954). CONCLUSION: The Real-Q HCV quantification kit showed a good specificity, sensitivity, linearity, and accuracy; therefore, we propose that it is fully adequate for monitoring antiviral therapy in patients with chronic HCV infection.