A case report of near-triploid in adult acute lymphocytic leukemia / 대한내과학회지

In addition to age, white cell count and immunophenotype, karyotype has been reported to be one of the important prognostic factors in acute lymphocytic leukemias.Furthermore 70 percent of patients with acute B lymphocytic leukemia presented chromosomal abnormalities, which is known to have a close relationship with the prognosis. Among the abnormalities, triploid is rare and known to have the worse prognosis. Structural chromosomal abnormality of the 11q23 band is more common in childhood acute lymphocytic leukemia and has been rarely reported in adult lymphocytic leukemia. We present a case of a 29 year - old male patient with acute lymphocytic leukemia, who had triploid and chromosomal translocation including 11q23 band along with the review of related literature.

A Case of Hereditary Factor VII Deficiency Performed Operation without any Replacement Therapy / 대한혈액학회지

Hereditary factor VII deficiency is a rare disorder transmitted by autosomal recessive pattern and its clinical feature is extremely variable. Recently we have experienced a case who was performed herniorrhaphy without any cryoprecipitate or fresh frozen plasma replacement therapy. A 21-year-old patient who had undergone intermittent nasal bleeding was noted for prolonged prothrombin time(INR of 1.5). The level of the factor VII of the patient was decreased at 22% of the normal activity. His parents had normal level of factor VII activity. Both his sister and brother had decreased level of factor VII at 41% and 24% respectively. Herniorrhaphy was performed without factor replacement therapy and there was no visible postoperative bleeding. We report here a case of factor VII deficiency performed an operation successfully without any replacement therapy.

A seroepidemiological study of anti-HAV IgG in korean combat policemen / 대한내과학회지

BACKGROUND: Hepaitis A virus(HAV) infection occurs commonly during early childhood. Recent improvements in sanitation and hygiene have resulted in a decrease in HAV infection among children, while the clinical illness of hepatitis A and prevalence of antibody to HAV(anti-HAV IgG) have been increased, particularly in the 3rd decade of young male adults. We studied a seroepidemiology of anti-HAV IgG in combat policemen who were living together during their service in the combat police force. METHODS: We measured anti-HAV IgG(HAVAB, Abbott) from 1,009 healthy subjects with no history of transfusion of any blood products during the last 6 months. The mean age was 20.8 years. RESULTS: Overall, anti-HAV IgG was detected in 30.2% of study subjects. According to age, the positive rates of antibody were 15.7%, 25.9%, 31.5%, 38.1%, 43.5%, and 50.0% in 19, 20, 21, 22, 23, and 24 years respectively. The positive rates of anti-HAV IgG as to period of service were 17.3%, 28.1%, 40.9% in 0 month, 1-12 months, 13-24 months respectively. The positive rates of anti-HAV IgG were 27.0% in the urban group, 39.8% in the rural group. CONCLUSION: The positive rates of anti-HAV IgG in our data low compared with those of previous reports. We think that group living condition such as living in dormitories or barracks can be a risk factors for hepatitis A infection and that improvements to the sanitary system and active immunization would be necessary for prevention of it.

Increased Growth of Human Leukemic HL-60 Cells by Dimethyl Sulfoxide and Dimethylformamide / 대한면역학회지

A recent study has proposed that dimethyl sulfoxide (DMSO) increases the number of HL-60 cells in high cell density conditions by inhibiting cell density-dependent apoptosis. We observed that dimethylformamide (DMF), a DMSO-related polar compound, also increased the concentration of HL-60 cells. The effective dose range of DMSO and DMF was 0.5-1% and 0.2-0.6% respectively. DMF, like DMSO, inhibited density-dependent apoptosis of HL-60 cells. The flow cytometric PKH26 cell proliferation assay showed DMSO and DMF actively increased cell division. However, the difference in the distribution of cell cycle phase was not noted between the control and the DMSO- or DMF-treated HL-60 cultures. Finally, DMSO and DMF stimulated HL-60 growth even in low density conditions. These results suggest that DMSO and OMF at appropriate concentrations increase the number of HL-60 cells by both apoptosis inhibition and cell division augmentation.