RESUMO
Background: Plants and their phytochemicals have immense potential for the management of different types of wound. Salvia multicaulis Vahl. is an evergreen shrub species, which traditionally used as a wound healing agent
Objective: The aim of the present study is to evaluate phytochemicals and wound healing activity of S. multicaulis in an excision wound model
Methods: Preliminary phytochemical screening tests were performed on the methanol [80%] extract. Total phenolic content was measured using Folin-Ciocalteu reagent. Antioxidant activity of the extract was assessed by [DPPH] free radical scavenging test. 2x2 excision wounds were induced in the dorsal area of 30 Wistar rats. Animals were received topical eucerine, 1% phenytoin as well as 5%, 10% and 20% ointments of the plant extract in eucerine base. Wound areas were photographed and wound contraction was calculated as the reduction percentage of wound size compared with zero days. Last day skin samples of wounded area were used for histological evaluations
Results: Collagen deposition for positive control group and A20% were low fibrils and A10% high fibrils and A5% highest fibrils. Total phenolic content was 262.083 +/- 47.15 mg GAE/g extract. IC50 was 54.02 micro g/mL. All concentrations of the extract ointments were significantly effective in the animal model of excision wound [P<0.05] which was also confirmed in histological study
Conclusion: It is concluded that S. multicaulis extract could act as a wound healing agent. This activity is possibly due the high concentrations of phenolic compounds
RESUMO
Apiaceae family is one of the largest plant families with wide distribution all around the world. The genus Hippomarathrum has 28 species around the world which among them the species H. microcarpum outspread in the North, Northwest, West and Central of Iran, and also Turkey, Caucasus and Iraq. In this study, the main compounds in ethyl acetate extract from aerial parts of the plant Hippomarathrum microcarpum have been studied. The isolation and purification of the compounds were carried out using column chromatography. The structure of isolated compound established according 1H-NMR and 13C-NMR spectral data. Two coumarins [osthole and isoimperatorin] and one sterol [beta-sitosterol] have been isolated and identified from ethyl acetate extract. The presence of coumarines like osthole and isoimperatorin in H. microcarpum cause of the importance of this plant. This coumarines have verity of usages in traditional medicine and recent studies have supported most of these usages and revealed new effects of these coumarines. As regard that more research haven't been done about this plant and also because of significance effects of isolated coumarines, it seems necessary to perform further studies on biological effects of this plant