trial to protect rabbits against psoroptes cuniculi mites by immunization with psoroptes extract

This study based on the evaluation and characterization of the immune response induced by Psoroptes cuniculi mite extract. For this purpose, eight rabbits wee used and divided into two groups. The first group composed of five rabbits was immunized subcutaneously with Psoroptes cuniculi extract emulsified with freund's adjuvant on day 0,14 and 28. The second group composed of three rabbits considered as control. All rabbits were challenged on day 35 post-immunization by placing live mites in external auditory canal. No clinical signs of Psoroptes mange were observed among immunized rabbits. Although few eggs were detected at third week post-challenge in immunized rabbits, but no developmental stages of mites were observed until the termination of the experiment. Analysis of with Psoroptes cuniculi antigen by SDS-PAGE revealed that there were 3 polypeptide bands with molecular weights 154.54 and 32 KDa. Immunoblot using hyper-immune sera from immunized rabbits recognized a polypeptide band with molecular weight 54 KDa

Parasitological, haematological and biochemical studies on naturally infected goat with theileria species in Egypt

The prevalence of theileria infection was investigated in 65 goats with suspected symptoms of theilerioses from a private farm in Giza Governorate. In addition, a total of 51 blood samples were collected from apparently healthy goats from private farms and ruing slaughtering at El-Bassatine abattoir. The incidence of theileria infection in the farm was 86.1% while, the incidence in the apparently healthy goats was 43.1%. Ring form of theileria species was detected in infected erythrocytes. Macroschizonts and microshizonts were detected in the lymphocytes with different shapes of chromatin materials. Haematological studies revealed significant decline in haemoglobin concentration, packed cell volume and red blood cell count. Total leucocyte count showed significant leucopenia in diseased animals. Examination of serum revealed significant rises in levels of alanine aminotransferase, aspartate aminotransferase, alkaline phophatase, urea, creatinine and cholesterol. Concentration of serum total protein, albumin and globulin showed significant decrease in theileria infected animals as compared with the healthy control goats. Levels of serum iron, inorganic phosphorus, magnesium and calcium showed also significant decline as compared with healthy goats. No changes were observed in serum sodium and triglycerides levels of goats with theilerioses. Therefore, it is important to put in consideration the great economical losses caused by theilerioses in such important small ruminants