Análisis bacteriológico de agua potable de los suburbios de Asunción / Annual Reports 1991 / Bacteriological analysis of drinking water in the suburbs of Asunción

A bacteriological study of drinking water in three peripheral neighborhood of Asunción was performed. the sample were taken at nine homes dwellers were asked a questionnaire. The sample were taken in sterile 1-liter bottles from: 1. Water kept in 50 lt-plastic buckests; 2. Directly from the tap. At the time of sample taking, several children have acute diarrhea, some of them with several days of duration. The sample were filtered through 0.45 mu Millipore membranes and the material retained on the filter was cultured on S.S agar medium and incubated at 37 ºc overnigth. On the other hand, 10 ml of the sample were cultured in alkaline peptone, pH 8.5, peptone broth and selenite borth and incubated at 37 ºc for 12 h. Subculture was performed in TCBS agar, EMB agar and Ss agar respectively. Biochemical characterization of the growing micoorganisms isolate were Salmonella ssp, Enterobacter ssp and Prosteus ssp. Six samples were negative for microorganisms

Estudio etiológico de la diarrea bacteriana en niños en Paraguay en 1990 / Annual Reports 1991 / An etiological study of bacterial diarrhea among infants in Paraguay in 1990

An etiological study of bacterial diarrhea in infants under five years of age in Paraguay from May to July of 1990 was done. Enteropathogenic E. coli (EPEC) strains of known serogroups were isolate from 35.8 percent of the patients with diarrhea, Salmonella spp. were isolated from 15.1 percent , enterotoxigenic E. coli (ETEC) strains were isolated from 5.7 percent, and Shigella and Campylobacter spp. were isolated. Out of 53 diarrheal patients, 34 cases (64.1 percent) had bacterial diarrheal disease. The infants under one year of age with diarrhea were predominant among impatient and outpatient children under five years of age and the total number of male diarrheal cases was greater than that of female cases. Enteropathogenic organisms were isolated from nine out of 12 water samplestaken from the Paraguay River. The major serotype (O18) of the EPEC isolated from the water samples of the Paraguay River correspond to the mayor serotype isolated from patients with diarrhea

Aplicación de dos tipos de amastigotes de Trypanosoma cruzi de difernte virulencia en una Elisa para la enfermedad de chagas / Annual Reports 1991 / Application of two types of Trypanosoma cruzi amastigotes of different virulencias to Elisa for Chagas' disease

Trypanosoma cruzi amastigotes derived from fibroblast cultures and from cell- free liquid mediun were comparatively evaluated as solid-phase of Elisa for Chagas' disease. Amastigotes were obtained from the high- and low-virulent clones of the Talahuen strain and fron the G-1 strain. They were sonicated and directly applied into wells of a microplate to use insoluble particles as antigens. Fifty-one sera from patients of Chagas' disease including one acute caes (Romaña's sing), 10 sera from patients of cutaneous leishmaniasis including one serun of midex infection with Chagas' disease, and 10 sera from patients of toxoplasmosis including three of mixed infection with either Chagas' disease or Leishmaniasis were examined. All amastigotes from different sources and from different clones or strains showed considerable correlation with each other. Slightly higher sensitivity was found in amastigotes of high-virulent clones and slightly higher specificity in those from cell-free culture. One acute case was detected as positive by these methods although it was negative by the usual Elisa using epimastigotes