RESUMO
Background/aim: the present study was conducted to assess the ameliorative role of Moringa oleifera leaf extract [MOLE] on genotoxicity and biochemical alteration of aflatoxin B1 [AFB1] in rats
Materials and methods: the rat groups involved negative control, control of DMSO, positive control that received AFB1 in DMSO [0.7 g/kg, body weight] four times weekly for 1 month, groups 4-6 that received the same dose of AFB1 in DMSO at the same period plus MOLE doses [3.3, 4.0 and 4.7 g/kg] daily for 1 month, and groups 7-9 that received MOLE alone at the same doses for 15 days after cessation of AFB1 treatment. Molecular genetic, cytogenetic, sperm, and biochemical studies were documented
Results: genetic and sperm results revealed that AFB1 treatment induced significant elevation of genetic alterations and sperm abnormalities as compared with normal control. Biochemical studies showed that the treatment with AFB1 disturbed the parameters of liver functions, where aspartate-transaminase, alanine-transaminase, and alkaline phosphatase were activated and bilirubin contents as well as the rate of malondialdehyde were increased significantly, but the endogenous antioxidative system [catalase, superoxide-dismutase activities and glutathione as well as total antioxidant capacity] and protein profile were reduced significantly. Moreover, kidney functions [urea, uric acid, and creatinine contents] were elevated under AFB1 administration. The treatment with MOLE significantly minimizes the genetic alterations, sperm abnormalities, and biochemical destruction. These ameliorations were increased by increasing the dose level. Better findings were seen by using MOLE as a therapeutic agent than its using as a protective agent
Conclusion: this study revealed that MOLE contains therapeutic factors used in curing of genotoxicity induced by AFB1 in rats, and treatment of animals that were exposed to AFB1 with MOLE significantly ameliorated the genetic, sperm, and biochemical parameters as compared with animals treated with AFB1 alone
RESUMO
Hydroquinone [HQ] is a myelotoxin that is found in many foods and is also formed through the metabolism of benzene. HQ is genotoxic in several in vitro and in vivo test systems, inducing micronuclei [MN], sister-chromatid exchange [SCE], and chromosomal aberrations. The aim of the current study was to explore the protective effect of Zizyphus jujuba and Origanum majorana extracts against HQ-induce genotoxicity and histological changes in male mice. Six groups of mice included the control group, HQ-treated group [125 mg/kg b.w] and the groups treated with the extracts alone[0.5g/kg b.w] or in combination with HQ. The results indicated that treatment with HQ resulted in significant clastogenetic effects and histological changes typical to those reported in the literature. Both extracts exhibited a protection against HQ-induced cytogenesis and histological changes. Moreover, Z. jujuba extract was effective than O. majorana extract. It could be concluded that both extracts may be useful for people especially those who occupationally exposed to benzene or its metabolites