RESUMO
Allopathic system of medication is the most popular and preferable approach of the treatment in the current scenarios, but in spite of the wide use of allopathic medicine, the trend of use of herbal medicines are also valuable contributor with the current medicinal therapies in the health care professions. In this connection, an eastern use of Morus nigra L. fruit was formulated into solid dosage from named as Lozenges. The lozenges consisted of Morus nigra L. aqueous extract, which dissolves in the mouth providing localized effect against sore throat and cough. A full-scale batch of lozenges trial was manufactured to ensure uniform process and analytical parameters. The un-packed lozenges were evaluated for qualitative specifications like, average weight which was about 2.250-2.750 [2.5 g +/- 10%] and uniformity of weight was 18/20-NMT +/- 10% 2/20. The results were found within the official limits. The appearance of the lozenges was checked organoleptically while dimensions were evaluated using calibrated vernier caliper. Packed and finished lozenges dosage form were evaluated for microbiological test like, aerobic bacteria, fungi, P.aeruginosa, E. coli, Staphylococcus aureus, Salmonella and other enteric bacteria. Thin Layer Chromatography [TLC] confirmed the presence of flavonoids as anthocyanins at 254 nm. The quantity of flavonoids as anthocyanin [cyaniding-3-glucoside] was determined as not less than [NLT] 0.80 mg/Loz. Caffeic acid was also estimated quantitatively using High Performance Thin Layer Chromatography [HPTLC] and was found NLT 0.002 mg/loz
RESUMO
Fluoroquinolones are broad-spectrum antibiotics, work against Gram-positive and Gram-negative bacteria and are a clinically proven option for many resistant infections. Among fluoroquinolones Levofloxacin works best against acute sinusitis, inflammation of the lower airways, acute exacerbation of chronic bronchitis, community acquired pneumonia, complicated urinary tract infection including Pyelonephritis, chronic bacterial prostatitis and skin and soft tissue infection. Levofloxacin is a frequently prescribed antibacterial agent with Diclofenac Sodium for pain management in infectious conditions. The objective of the present work is to evaluate the level of interaction between Levofloxacin and Diclofenac Sodium. In this work market available brands of both drugs were also evaluated for quality. The physiochemical parameters like weight variation, thickness variation, and mechanical strength were determined. Similarly the percentage drug release and content uniformity test were also analyzed; the tested quality attributes were found within the recommended pharmacopeia ranges except brand L6 that had high drug content 124.629 +/- 3.614 while brand L[4] and L[5] were not found similar in pH 1.2. When subjected to model dependent analysis Levofloxacin showed compliance with [first order, Higuchi, Hixson Crowell and Weibull] at pH [1.2, 4.5 and 6.8]. However Diclofenac Sodium showed adherence with [first order, Hixson Crowell and Weibull] at pH [1.2, 4.5 and 6.8] but following Higuchi at pH 1.2 and 4.5 only. The interaction studies were also performed spectrophotometrically and simultaneous equation was used to estimate the percentage availability of both the drugs at pH 4.5, 6.8, FaSSGF and FaSSIF. The studies showed that the percent availability of Levofloxacin was increased significantly in FaSSIF i.e. 129.173 +/- 0.323 at 45 minutes in the presence of Diclofenac Sodium