RESUMO
Objectives: This study aimed to establish lactate dehydrogenase [LDH] and Beta-glucuronidase as salivary biomarkers of periodontitis among smokers and non-smokers
Methods: This cross-sectional case-control study was conducted at the Aligarh Muslim University, Aligarh, India, between January and June 2017. A total of 200 participants were divided into four groups based on their periodontal and smoking statuses. Unstimulated mixed saliva samples were collected to estimate LDH and Beta-glucuronidase levels. In addition, total protein was estimated using Lowry's method
Results: There was a significant increase in enzyme activity in the periodontitis groups compared to the nonperiodontitis groups [P <0.001]. However, significantly lower enzyme activity was observed among smokers, irrespective of periodontal status [P <0.001]. Nevertheless, a receiver operating characteristic curve analysis indicated the diagnostic potential of both enzymes to be fair-to-excellent
Conclusion: Although smoking was found to significantly alter enzyme activity, LDH and â-glucuronidase were reliable salivary biomarkers of periodontitis among both smokers and nonsmokers