Cytocompatibility and cell proliferation evaluation of calcium phosphate-based root canal sealers

OBJECTIVES@#This study aimed to evaluate the cell viability and migration of Endosequence Bioceramic Root Canal Sealer (BC Sealer) compared to MTA Fillapex and AH Plus.@*MATERIALS AND METHODS@#BC Sealer, MTA Fillapex, and AH Plus were placed in contact with culture medium to obtain sealers extracts in dilution 1:1, 1:2 and 1:4. 3T3 cells were plated and exposed to the extracts. Cell viability and migration were assessed by 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide (MTT) and Scratch assay, respectively. Data were analyzed by Kruskal-Wallis and Dunn's test (p 0.05) and MTA Fillapex was more cytotoxic than BC Sealer (p 0.05).@*CONCLUSIONS@#All tested sealers demonstrated cell viability highlighting BC Sealer, which showed increased cell migration capacity suggesting that this sealer may achieve better tissue repair when compared to other tested sealers.

Cytocompatibility and cell proliferation evaluation of calcium phosphate-based root canal sealers

OBJECTIVES: This study aimed to evaluate the cell viability and migration of Endosequence Bioceramic Root Canal Sealer (BC Sealer) compared to MTA Fillapex and AH Plus.MATERIALS AND METHODS: BC Sealer, MTA Fillapex, and AH Plus were placed in contact with culture medium to obtain sealers extracts in dilution 1:1, 1:2 and 1:4. 3T3 cells were plated and exposed to the extracts. Cell viability and migration were assessed by 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide (MTT) and Scratch assay, respectively. Data were analyzed by Kruskal-Wallis and Dunn's test (p < 0.05).RESULTS: The MTT assay revealed greater cytotoxicity for AH Plus and MTA Fillapex at 1:1 dilution when compared to control (p < 0.05). At 1:2 and 1:4 dilutions, all sealers were similar to control (p > 0.05) and MTA Fillapex was more cytotoxic than BC Sealer (p < 0.05). Scratch assay demonstrated the continuous closure of the wound according to time. At 30 hours, the control group presented closure of the wound (p < 0.05). At 36 hours, only BC Sealer presented the closure when compared to AH Plus and MTA Fillapex (p < 0.05). At 42 hours, AH Plus and MTA Fillapex showed a wound healing (p > 0.05).CONCLUSIONS: All tested sealers demonstrated cell viability highlighting BC Sealer, which showed increased cell migration capacity suggesting that this sealer may achieve better tissue repair when compared to other tested sealers.

Avaliação do tecido peri-implantar comparando as técnicas com retalho e sem retalho: revisão da literatura / Evaluation of peri-implant tissue comparing techniques with flap and flapless: literature review

Na tentativa de preservação dos tecidos mole e duro, a implantodontia tem optado por uma abordagem cirúrgica sem retalho (técnica flapless). Essa técnica minimamente invasiva destaca-se por suas vantagens como menor sangramento durante a cirurgia, menor tempo cirúrgico e melhor pós-operatório para o paciente. Diante disto, o objetivo desta revisão de literatura é trazer informações sobre o comportamento dos tecidos Peri-implantares nas reabilitações implantossuportadas bem como comparar as técnicas cirúrgicas com e sem retalho. Realizou-se uma busca na base de dados Pubmed/Medline e foram incluídos na revisão estudos clínicos controlados que apresentaram relevância clínica publicados no período de 2008 a 2013. De acordo com os resultados, pode-se observar que a técnica sem retalho proporciona a manutenção da mucosa peri-implantar ao redor dos implantes. Entretanto, não existe diferença significativa quanto à perda óssea entre as técnicas estudadas. Além disso, ambas apresentaram previsibilidade nos resultados do tratamento com implantes. Pode-se concluir que não existem estudos clínicos controlados em longo prazo suficientes quanto à utilização da técnica flapless, entratanto, os resultados em curto prazo sugerem que é uma técnica previsível

In an attempt to preserve the soft and hard tissues, implant dentistry has opted for a flapless surgical approach (flapless technique). This minimally invasive technique is distinguished by its advantages such as less bleeding during surgery, shorter operative time and best postoperative for the patient. Hence, the purpose of this literature review is to provide information about the behavior of the peri-implant tissues in implant restorations and to compare surgical techniques with and without flaps. A search was conducted in the database PubMed/Medline and controlled clinical trials that showed clinical relevance published in the period 2008-2013 were included in this review. According to the results, it could be observed that the flapless surgical approach provides maintenance of the peri-implant mucosa around implants. However, no significant difference in bone loss between techniques was also observed. Besides that, both techniques, with or without flaps, showed predictability in the results of implant treatment. It can be concluded that there are not enough controlled clinical studies regarding the long term use of flapless technique. However, short term results suggest that it is a predictable technique