Extraction de l'ADN chromosomique de staphylococcus et de listeria par une methode rapide a l'achromopeptidase

A rapid and simple method for preparation of chromosomal DNA from Gram-positive bacteria is reported. Susceptibility to lyses with Sodium Dodecyl Sulfate [SDS] increases when undergoing treatment with acetone before being digested by bacteriolytic enzymes. Rapid lyses of Staphylococcus and Listeria cells is obtained through a respective treatment by lysozyme with lysostaphine and by lysozyme with achromopeptidase, adding to that the effect of SDS in Tris-HcI buffer. This procedure of preparing chromosomal DNA provides to 4 mg of DNA out of 1 g of bacterial cells in a day