[Glycodelin expression in human breast cancer]

Glycodelin expression in normal and cancerous human breast tissue and its relation with age, tumor type, microscopic grade and metastasis to axillary lymph nodes recently were noticed. This study was done to evaluate the glycodelin expression in breast cancer. In this descriptive study, 96 Paraffin-embedded blocks of malignant breast cancer by immunohistochemistry method were considered to evaluate the expression of glycodelin. Patients age,tumor size, tumor type, microscopic grade and metastasis to axillary lymph nodes were recorded for each subject. Glycodelin was found in 30.45% of invasive carcinoma of the breast with axillary lymph node metastasis. Glycodelin was expressed in 72.7% of carcinoma of the breast without lymph nodes metastasis [P<0.05]. There was a significant relation between glycodelin expression with microscopic grade 1 of tumor and metastasis to less than 4 of lymph nodes [P<0.05]. Glycodelin expression can be evidence for lack of axillary lymph node metastasis of breast cancer

Assessment the diagnostic validity and the relationship between urinary fsh and serum fsh in menopausic and non-menopausic women

Background and purpose: FSH [Follicle stimulating Hormone] is a glycopeptid hormone released from anterior pituitary and is associated with ovarian function. Due to its daily and monthly cyclicity, successive sampling is used for certainty. Because of the kydney intervention in gradual releasing FSH, random and morning urinary sample was considered in menopausic and non-menopausic women

Methods and Materials: 50 woman with ages 35-60 years old participated in the study. The participants had not ured oral contraceptives, had normal renal function and were admitted for hysterectomy, due to benign illnesses, at Imam Khomeini Hospital in Sari, Iran. Frequcut sampling and random blood sample were taken a day before surgery by research aids blind to the participants menopausal status. FSH was measured through radio immunoassay and its correlation [gold standard] was obtained with each urinary sample in both groups. Sensitivity and specificity was studied

Results: Out of 47 cases admitted, 37 cases were monmenopausic with FSH less than 20 mlu/ml and 10 cases were menopausic with FSH more than 40 mlu/ml; 3 cases were ruled out because their FSH levels were not ascertained. FSh correlation with random and morning urinary samples were 31% and 84% respectiviely in nou-monopausic cases. In the second group, 68% and 77% were observed respectively. The sensitivity and specifity in random urine sample were 100% and 97% respectively; while in morning samples, they were both 100%

Conclusion: According to the findings, high correlations were observed between serum FSH and morning sample in menopausic cases, as well as with random sample. Also because of the satisfactory specificity and sensitivity of FSH measurement with urine samples, it is possible to replace floquent sampling with one morning sample in pathological and physiological instances. This method is non- invasive, simple and cheap

[Toxicity effects on aluminium chloride on uterus and placenta of pregnant mice]

Aluminium has been known to cause toxic effects on organ systems. Although the knowledge on aluminium [AL] toxicity has markedly improved in recent years, information concerning the reproductive toxicity of this element is still very limited. The purpose of this study was to assess the effect of graded dose of aluminium chloride administered to pregnant mice on their placenta and uterine in a short time. This study was performed on plug-positive female NMRI [National Medical Research Institute] mice that were randomly divided into six groups [12 mice in each group]. Three groups of plug- positive female NMRI mice were given IP injections of ALCL[3] at 150 mg/kg on days 10, 11 and 12 of gestation, respectively. Three other groups were given normal saline on the same days as control groups. Mice were killed on day 15 of gestation, the weight and diameter of placenta were recorded. Both placenta and uterus were examined with stereomicroscope and also uterus fixed and stained for microscopic examination. In all experimental groups, in 14.3%, 13.8% and 13.3% of fetuses, placenta showed abnormal appearance and some degrees of atrophy on days 10, 11, 12 of gestation, respectively that in comparison to control group it was significantly higher [p<0.05]. Also, the diameter of placenta in experimental groups [7.2, 7.2 and 7.3mm respectively] was significantly smaller than control groups [7.6mm] [p<0.05]. The uterine of All treated groups showed hemorrhagic area in external examination and infiltration reaction and dispersed nectrotic foci in myometer in microscopic examination. According to the results, AlCl [3] showed toxic effect on placenta and uterus of pregnant mice. It could explain at least part of teratogenic effects of AlCl [3]