Increase serum IL-8 level in Iraqi patients with helicobacter pylori CAG A genotype infection

More than half of all humans are colonized in their stomachs by Helicobacter pylori. That carriage was nearly universal among adults in developing countries suggests that in earlier times most humans carried these organisms. H. pylori was an important human pathogen that causes gastritis and is strongly associated with gastric ulcers, gastric adenocarcinomas, and mucosa-associated lymphoid tissue lymphomas. In response to [I. pylon, interleukun-8 [IL-8] was secreted from host cells to attract components of the innate and adaptive immune systems to the site of infection. Cag A cells induce higher levels of the proinflammatory IIL-8 cytokine. That such effects are specific for Cag A island genes had been shown by our study. To determine the role of incidence of H. pylori Cag A positive strain in induce higher level of the proinflammatory cytokine IL-8. One hundred and fifty [150] Patients attended the Endoseopic Unit at Gastroenterology and Hepatology Teaching Hospital/ Baghdad Medical City were included in this study with ages range from 18 years to 65 years. The source of specimens to undergo oesophageal gastroduodenoscopy [DOD] collected from April 2009 to end of March 2010 were eligible for this study. Two types biopsy of samples for histopathology to detect H. pylori and detection of Cag A gene by In Situ hybridization[TSH] and blood samples for estimation of serum IL-8 level by ELISA. There was a significant increase in IL-8 serum levels that P<0.05 due to the significant increase of Cog A in H. pylori positive strains. Our study revealed that increased in Cag A expression in H. pylori positive strains and induced higher levels of the proinflammatomy eytokines

Activation of complements pathways in patients with Bechcet's disease

Behcet's disease [BD] is amultisystemic inflammatory disorder, onset is belived to be triggered by many factors with a particular immunogenetic Factor. The aim of this study is elucidate the role of C3,C4 complement components With CH50 and HLA typing as immunogenetic factor in aetiopathogenesis of this disease. Seventy patients with Behcet's disease have been studied to assess the level of serum complement's components [C3,C4], complement total hemolytic activity [CH50], and HLA- typing using single radial immunodifusion [SRID], titration assay, and microlymphocytotoxicity methods respectively, compared with age, sex and ethic matched 30 patients control with recurred oral ulcer, 25 healthy relative, and 30 healthy volunteer. Significant elevation [P<0.01,0.05,0.0005] in C3, C4 and CH50 levels was clearly observed in patients with BD, especially those who show to be HLA-B51[5] positive in comparison to control groups. Increased level ofCH50 which is reflected the total activity of the classical and lytic pathways, elevation of C3 level is an index of the disease activities which support that alternative pathway play a role in the pathogenesis of BD in Iraqi patients especially in those with HAL - B51[5] positive

Possible association of HLA Class-1 molecules with rheumatoid arthritis in Iraqi Patients

Rheumatoid Arthritis [RA] is a multisystem inflammatory disease, which primarily affects synovial joints. to shed light on the possible association o] HLA class-l antigens in RA patients in Iraq. Microlymphocytotoxicity assay has been used to assess HLA-typing of 249 blood samples of 80 RA patients, 62 patient controls [SLE] and 107 healthy normal controls. This study revealed that HLA-A10, B22, B40, Cwl and Cw7 might play a role in RA susceptibility with significant differences between RA and controls [P<0.0001, 0.009, 0.007, 0.005 and 0.002]. On the other hand negative association was observed significantly with HLA-A2 [P<0.0001] and B14 [P<0.028]. high RF positivity participated in increasing the inflammatory effects which probably reflects the acute phase response by high level of CRP as this study pointed to