RESUMO
We evaluated the safety of fucoidan from Gagome Kombu (GKF) in genotoxicity tests. In bacterial reverse mutation test, GKF had no reverse mutation inducing activity on five bacterial strains with or without S9 metabolic activation. In chromosome aberration test, GKF had neither structural nor numerical chromosome aberration inducibility with or without S9 metabolic activation. In micronucleus test, neither formation of micronuclei nor decrease of mironucleated reticulocytes was observed in the bone marrow of the mice treated by GKF. These results indicate that GKF has no genotoxic activities under the condition of this study.<br>
RESUMO
<i>Coix lacryma-jobi </i>L. var<i>.ma-yuen</i> Stapf (coix seed) is a grass crop that has long been used in traditional medicine as a nourishing food. However, high-intake safety of the extract of the husks, pellicles and astringent skin of coix seed has rarely been evaluated. We performed a 28-day repeated dose oral toxicity test of hot water extract of all parts of Coix seed in rats. The extract showed no significant toxicity on body weight, blood analyses, urinalysis and histopathological examination in acute toxicity tests.<br>
RESUMO
<i>Coix lacryma-jobi</i> L. <i>var. ma-yuen</i> Stapf is a grass long been used in traditional medicine as a nourishing food and reported to possess pharmacological effects including anti-tumor, anti-obesity, anti-diabetic, etc. In order to evaluate the possible mutagenicity of the hot water extract of all parts (husks, pellicles, and astringent skin) of the food, we performed a reverse mutation test in bacteria, a mouse lymphoma assay and a mouse micronucleus test. The results of all tests were negative. It was concluded that the extract has no mutagenicity for living bodies.<br>