RESUMO
<p><b>OBJECTIVE</b>To investigate the effects of PHI on histone acetylation and methylation in hepatocellular carcinoma line SMMC-7721 cells.</p><p><b>METHODS</b>Apoptosis was measured by TUNNEL assay. Histone methylation and acetylation were detected by Western blot.</p><p><b>RESULTS</b>PHI inhibited cells growth and induced apoptosis. PHI treatment resulted in increased acetylation of histone H3 and H4 , elevated level of histone H3 lysine 4 methylation, and decreased level of histone H3 lysine 9 methylation.</p><p><b>CONCLUSIONS</b>PHI can modulate both histone acetylation and methylation, which could remodel chromatin structure. PHI may be a novel anticancer drug.</p>
Assuntos
Humanos , Acetilação , Apoptose , Linhagem Celular Tumoral , Proliferação de Células , Histonas , Metabolismo , Isotiocianatos , Farmacologia , MetilaçãoRESUMO
<p><b>OBJECTIVE</b>To investigate the effect of phenyl-hexyl isothiocyanate (PHI) on acetylation and methylation of histone in acute lymphoblastic leukemia cell line Molt4.</p><p><b>METHODS</b>The inhibition of cell proliferation was observed by MTT method and clone suppression test. Apoptosis and cell cycle arrest were measured by flow cytometry. The alterations in histone acetyltransferase and acetylation and methylation of histones were detected by Western blot.</p><p><b>RESULTS</b>PHI could up-regulate the expression of acetyltransferase (P300/CBP), markedly induced the accumulation of acetylated histone H3, H4 and methylated histone H3 lysine 4 (H3K4), and inhibited methylation on lysine 9 of H3 (H3K9). The epigenetic regulation resulted in cell cycle arrest at G0/G1 phase, and induction of apoptosis.</p><p><b>CONCLUSIONS</b>PHI can modulate both histone methylation and acetylation. It may serve as a histone deacetylase inhibitor, and might be a potential novel anti-leukemia agent.</p>